Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
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14:25 min
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December 12th, 2017
DOI :
December 12th, 2017
•Rozdziały w tym wideo
0:05
Title
0:46
Expression of His6-TIp3-LBD in E. coli
2:29
Isolation and Denaturation of Inclusion Bodies
5:34
Protein Refolding
7:39
Purification of His6-tagged Protein Using Immobilized Metal Ion Affinity Chromatogrphy
8:49
His6-tag Removal Using TEV Protease and Size-excluson Chromatography of Tlp3-LBD
11:06
Results: Expression, Refolding, and Purification of Tlp3-LBD
12:41
Conclusion
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