Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
Please note that all translations are automatically generated. Click here for the English version.
8.8K Views
•
12:04 min
•
October 3rd, 2018
DOI :
October 3rd, 2018
•Maria Felicia Soluri1, Simone Puccio2, Giada Caredda2, Giorgio Grillo3, Vito Flavio Licciulli3, Arianna Consiglio3, Paolo Edomi4, Claudio Santoro1, Daniele Sblattero4, Clelia Peano5,6
1Department of Health Sciences, Università del Piemonte Orientale & IRCAD, Novara, Italy, 2Institute of Biomedical Technologies, National Research Council, Segrate, Milan, Italy, 3Institute of Biomedical Technologies, National Research Council, Bari, Italy, 4Department of Life Sciences, University of Trieste, Italy, 5Institute of Genetic and Biomedical Research, National Research Council, Rozzano, Milan, Italy, 6Humanitas Clinical and Research Center, Rozzano, Milan, Italy
Rozdziały w tym wideo
0:04
Title
1:19
Construction of the ORF Library
2:38
Preparation of the Filtering Vector
3:40
Ligation and Transformation
6:47
pFILTER-ORF Library Validation and Collection
9:10
Phage Library Deep Sequencing Platform, Index PCR Followed by Qualitative and Quantitative Evaluation of the Sequencing Library
10:07
Results: Use of a Phagemid Vector to Construct, Characterize and Select a Domainome Library
11:23
Conclusion
Powiązane Filmy
11:14
Primer-Free Aptamer Selection Using A Random DNA Library
24.6K Views
07:53
Peptides from Phage Display Library Modulate Gene Expression in Mesenchymal Cells and Potentiate Osteogenesis in Unicortical Bone Defects
18.0K Views
12:42
Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity
12.2K Views
12:36
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
33.8K Views
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
83.2K Views
08:38
Targeted DNA Methylation Analysis by Next-generation Sequencing
36.9K Views
09:31
Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
17.5K Views
12:59
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
17.8K Views
12:01
3' End Sequencing Library Preparation with A-seq2
10.4K Views
08:05
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
19.4K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone