Zaloguj się

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

6.1K Views

07:03 min

January 2nd, 2018

DOI :

10.3791/56423-v

January 2nd, 2018


Przeglądaj więcej filmów

Genome wide

Rozdziały w tym wideo

0:05

Title

0:38

ActD Time Course

2:13

Isolation of Total RNA from Phenol-guanidine Isothiocyanate Solution Lysate

5:16

Analysis of Transcript Abundance

6:02

Results: Clear Contrast in Transcript Decay Between Proliferating and Quiescent Fibroblasts

6:28

Conclusion

Powiązane Filmy

article

12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

13.4K Views

article

09:59

Investigation of RNA Synthesis Using 5-Bromouridine Labelling and Immunoprecipitation

9.4K Views

article

09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

9.0K Views

article

10:49

Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells

5.9K Views

article

12:06

Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture

8.5K Views

article

00:11

Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

10.7K Views

article

08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

3.4K Views

article

11:22

Measuring the Kinetics of mRNA Transcription in Single Living Cells

15.5K Views

article

11:00

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

27.0K Views

article

11:08

Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

12.5K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone