A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
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10:07 min
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August 25th, 2017
DOI :
August 25th, 2017
•Rozdziały w tym wideo
0:05
Title
0:38
Cell Line, Culture Conditions, and Harvest
1:57
Plating the Cells and Releasing Them from Aphidicolin Synchronization
2:44
RNA Complexing
4:13
Harvesting the Cells for Targeting
5:30
Targeting Samples
6:13
Analysis of Gene Edited Cells and Transfection Efficiency
6:57
DNA Sequence Analysis
7:56
Results: Effective Point Mutation Repair
9:41
Conclusion
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