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Rapid Deletion Production in Fungi via Agrobacterium Mediated Transformation of OSCAR Deletion Constructs

9.9K Views

11:24 min

June 12th, 2017

DOI :

10.3791/55239-v

June 12th, 2017


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Rapid Deletion Production

Rozdziały w tym wideo

0:05

Title

1:02

Primer Design for PCR Amplification of Gene Flanks

10:23

Conclusion

8:53

Results: The OSCAR Method Generates a Gene Deletion Plasmid for Fungi in a Single Reaction

2:49

Production of OSCAR Constructs

5:48

Agrobacterium tumefaciens Mediated Transformation (ATMT) of Fungi

7:45

Deletion Mutant Identification by PCR

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