Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

0:47

Transfection of Adherent HEK293S GnTI-cells for Thermostability Screen

2:24

Scintillation Proximity-based Thermostability Screen with Citalopram

4:18

Expression of the Human Serotonin Transporter in HEK293S GnTI-cells

5:24

Affinity-purification of the Serotonin Transporter for Immunization and Crystallization

7:12

Formation of Transporter-antibody Complexes and Separation by Size-exclusion Chromatography

8:21

Crystallization of Transporter-antibody Complexes by Hanging Drop

10:01

Results: Thermostabilization and Crystallization of the Serotonin Transporter in an Antidepressant-bound State

11:25

Conclusion