HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
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11:29 min
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July 20th, 2016
DOI :
July 20th, 2016
•Rozdziały w tym wideo
0:05
Title
0:59
Isolation of Leukemic Lymphocytes from Blood Samples of CCL Patients
2:09
Purification of Leukemic B Cells by Negative Isolation
3:27
Program the HPLC Method
5:07
Pretreatment with the Inhibitors and Incubation with the Substrate (AMP)
6:16
Samples Preparation for HPLC and HPLC Measurements of Purines
8:14
Results: Quantification of Purine Compounds in Freshly Purified CLL Cells from a Representative CD73+ Patient
9:36
Conclusion
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