A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
Please note that all translations are automatically generated. Click here for the English version.
10.8K Views
•
11:36 min
•
July 3rd, 2016
DOI :
July 3rd, 2016
•Rozdziały w tym wideo
0:05
Title
0:43
Synthesis of NNS Sub-libraries for Each Amino Acid Position by Two-step PCR Site-directed Mutagenesis
1:53
Cloning of NNS Sub-libraries into Selection Vectors
3:47
Selection of the TEM-1 Whole-Protein Saturation Mutagenesis Library for Antibiotic Resistance
5:51
High Throughput Sequencing to Determine Fitness Effects of Mutations
8:44
Results: High Throughput Sequencing from Whole-Protein Saturation Mutagenesis
10:16
Conclusion
Powiązane Filmy
11:15
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
24.2K Views
10:41
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
11.6K Views
11:49
A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
6.5K Views
09:06
High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)
10.1K Views
08:16
Comparative Lesions Analysis Through a Targeted Sequencing Approach
6.7K Views
09:01
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
30.4K Views
11:59
Competitive Genomic Screens of Barcoded Yeast Libraries
18.2K Views
11:02
Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
19.4K Views
08:07
Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
8.0K Views
08:15
gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
12.2K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone