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The MultiBac Protein Complex Production Platform at the EMBL

15.9K Views

13:51 min

July 11th, 2013

DOI :

10.3791/50159-v

July 11th, 2013


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Keywords Protein Complex

Rozdziały w tym wideo

0:05

Title

2:13

Tandem Recombineering (TR) for Creating Multigene Expression Constructs

4:57

Composite Multigene Baculovirus Generation, Amplification and Storage

5:02

Baculovirus Generation

6:42

Amplification

8:14

Storage

8:55

Protein Production and Downstream Processing

8:57

Infecting Larger Cultures and Monitoring YFP

10:20

Cell Lysis and Fractionation

10:45

Protein Purification

11:07

Future Outlook

11:33

Results: Production of Eukaryotic Protein Complexes by MultiBac

12:18

Conclusion

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