Multi-target Parallel Processing Approach for Gene-to-structure Determination of the Influenza Polymerase PB2 Subunit
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22:10 min
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June 28th, 2013
DOI :
June 28th, 2013
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Rozdziały w tym wideo
0:05
Title
2:20
Construct Design
4:07
Polymerase Incomplete Primer Extension (PIPE) Cloning
6:14
Expression Optimization
8:21
Large Scale Fermentation
9:40
Protein Purification - Cell Lysis
10:54
Protein Purification - Affinity Chromatography
13:04
Protein Purification - Affinity Tag Cleavage
14:47
Protein Purification - Size-exclusion Chromatography (SEC)
15:42
Protein Crystallization
17:03
Crystal Harvesting
18:15
Crystal Screening/Data Collection
19:27
Results: 3D Structure Determination of Influenza Virus PB2
21:05
Conclusion
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