Zaloguj się

Single Read and Paired End mRNA-Seq Illumina Libraries from 10 Nanograms Total RNA

39.1K Views

14:49 min

October 27th, 2011

DOI :

10.3791/3340-v

October 27th, 2011


Przeglądaj więcej filmów

MRNA Seq

Rozdziały w tym wideo

0:05

Title

2:02

Procedural Flow Chart

3:19

cDNA Synthesis

6:31

End Repair, Paired End Library

7:37

Adding 'A' Bases to the 3' End of the DNA Fragments

8:31

Adapter Ligation

10:32

Size Selection/Gel Purification

11:20

Evaluation of Single-Read and Paired-End Sequencing Libraries

14:18

Conclusion

Powiązane Filmy

article

12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

13.4K Views

article

10:55

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells

8.0K Views

article

00:09

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

7.2K Views

article

08:49

Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

7.5K Views

article

05:12

AQRNA-seq for Quantifying Small RNAs

630 Views

article

12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

8.0K Views

article

08:37

Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos

5.3K Views

article

14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

11.8K Views

article

12:01

3' End Sequencing Library Preparation with A-seq2

10.4K Views

article

09:54

RNA-Seq

71.0K Views

JoVE Logo

Prywatność

Warunki Korzystania

Zasady

Badania

Edukacja

O JoVE

Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone