The University of California, San Francisco, and San Francisco Veterans Affairs Health Care System

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Live Cell Imaging with Time Lapse Photography to Study Epidermal Keratinocyte Proliferation Kinetics

Brook Abegaze¹, Giselle Vitcov², Richard Kim³, Ruby Ghadially¹

¹The University of California, San Francisco, and San Francisco Veterans Affairs Health Care System, ²The University of Arizona College of Medicine, ³Department of Medicine, Memorial Sloan Kettering Cancer Center

Here, we provide a method for live cell imaging analysis that can be used to manually track the lineages of passage 0 keratinocytes and that allows the collection of proliferation metrics, including cell division fate and cell cycle duration.