이 작품은 R21CA134819 (CH), T32CA009035 (SD) 및 P30CA006927 (암 센터 코어 그랜트)에 의해 재정 지원되었다. 저자는 원고의 준비와 함께 그녀의 도움으로 참가자 모집, 리아 보일, 에바 Panigrahi 및 절차의 개발에 그들의 도움 케이트 메네 제스와 함께 그녀의 도움 엘리자베스 Culnan 감사 드리고, 그리고 쟌느 Pomenti 것이다. 우리는 또한 그들의 도움을 제안 저널 리뷰어 감사합니다.

1. UV 노출을위한 준비 <ol><li> MED 테스트가 어떻게 작동하는지 참가자 ( &quot;나는 약 20 분, 내일의 과정을 통해 UV 빛을 당신의 팔에 약간의 피부를 노출하는거야 설명 할, 우리는 결정하기 위하여 당신의 피부의 해당 섹션을 확인하는거야 얼마나 민감한 당신은 빛입니다. 당신은 우리가 UV에 노출되는 작은 지역에서 햇볕이 발생할 수 있습니다. 햇볕이 고통 또는 당신을 귀찮게하는 경우, 당신은 다른 햇볕 것처럼 처리 할 수​​ 있습니다. &quot;) </li><li> 직원과 참가자 UV 보호 안경을 착용해야합니다. </li><li> 참가자는 반소매 셔츠를 입고 나 그녀의 소매를 걷어해야합니다. </li><li> 참가자가 그녀의 손에 피부를 보호하기 위해 장갑에 넣어 수 있습니다. </li><li> 패치의 왼쪽과 오른쪽에있는 Daavlin 패치 1 지원을 제거하고 장갑에 바로 인접한 안 팔 아래에 배치, 기존 피부 결점을 피하고. </li><li> 팔 위스콘신에서 추가 스킨 커버참가자의 셔츠 또는 기타 자료 일. </li><li> 패치 구멍이 UV 광원에 노출 될 수 있도록 팔을 놓습니다. 다른 피부 UV 빛에 노출되지 않아야합니다. </li><li> 그녀는 따뜻함하지만 그녀의 팔을 테스트하는 동안 레코딩되지 않습니다 느낄 것이다 참가자를 말한다. </li></ol> 2. UV 노출을 실시 <ol><li> 패치 구멍 (예 : 6) 노출 기간 동안 간격으로 자외선에 노출되어야한다. </li><li> 구멍 1 오픈과 함께 시작합니다. </li><li> 2. 공평한 화상의 가능성이 더 높습니다. 제조업체 광원의 스펙 및 참가자의 피츠 패트릭 피부 타입 I-VI (피츠 패 트릭, 1988 매우 어두운 매우 공정)에 따라 노출의 총 시간을 선택합니다 </li><li> 노출 (예 : 20 분)의 총 시간에 대한 타이머를 설정합니다. 두 번째 백업 타이머도 사용할 수 있습니다. 타이머 (들)을 시작합니다. </li><li> 4 후 예를 들어, 오픈 2 분 후, 구멍이 열려 구멍 3분, 8 분, 16 분 후 12 분, 및 오픈 구멍 6 후 열기 구멍 5 후 열기 구멍 4. 따라서, 피부 자외선 노출 시간 구멍 5 8 분, 및 구멍 6 4 분 홀 1 홀 2 홀 3 홀 4, 12 분 16 분 18 분 20 분 될 것입니다. </li><li> 위해서는 더 쉽게 24-48 시간 후 노출 된 영역을 식별하는 패치의 처음과 마지막 홀까지 가장자리에 노출 된 피부를 표시하고 피부가 검사 될 때까지 표시를 씻어하지 않는 참가자를 부탁드립니다. </li><li> 참가자는 장갑 및 패치를 제거했다. </li><li> 피부가 24-48 시간에서 재검토해야한다는 참가자에게 유지한다. </li></ol> 3. MED를 평가 <ol><li> 24-48 시간 후, 피부의 노출 부분을 검사합니다. 빨간색 또는 분홍색 피부 홍반 또는 연소를 나타냅니다. UV의 짧은 기간에 노출 Erythemetous 피부의 최소 홍반 량 또는 MED로 정의됩니다. </li><li> UV 향후 노출은 t보다 짧은 기간을 위해해야​​한다그는 연소 방지하기 위해 MED. </li><li> 노출 영역을 식별하기 어려운 경우, 노출 된 피부에 맞춰 마크를 사용하여 다시 패치를 넣을 수 있습니다. 이 또한 분광 광도계로 피부 색깔 측정 장치를 사용하는 경우 유용합니다. </li><li> 분광 광도계는 L의 측정 * (농도)를 제공하고, B * (색조). *이 피부의 홍조를 말합니다. 높은 * 값이 붉어 피부를 나타냅니다. </li><li> 이러한 분광 광도계로 피부 색깔 측정 장치를 사용하는 경우, 측정 할 구멍의 중앙에 분광 광도계 조리개를 배치합니다. </li><li> 숫자 순서와 비교를 위해 다른 가까운 한 노출되지 않은 영역에서 6 노출 각 영역을 측정합니다. 각 구멍의 중심 아니지만 주근깨 또는 두더지 또는 다른 비 UV 변색을 측정하려고합니다. 측정 각 레이블을 지정합니다. 노출되지 않은 영역에서 측정 0 분 표시되어야하며 지역 6 (짧은 노출 영역 - 4 분) 옆에 나열됩니다. </li><li> * 값의 증가는 UV 노출 시간의 증가와 일치해야합니다. 해당 순서에없는 다시 측정 값을보십시오. </li><li> 노출 된 피부에 비해 노출되지 않은 피부의 *에 2.5 점 차이가 잠재적 인 레코딩을 제안, 빨갛게에 상당한 차이를 나타냅니다. </li><li> 낮은 * 값에 2.5를 추가합니다. 이 값에 이상 아무것도 잠재적 연소 간주됩니다. 이 값보다 낮은 노출 시간은 MED 간주됩니다. </li></ol>

<ol>
	<li>Fitzpatrick, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+validity+and+practicality+of+sun-reactive+skin+types+I+through+VI.">The validity and practicality of sun-reactive skin types I through VI.</a> Archives of Dermatology. 124, 869-871 (1988).</li><li>Bodekaer, M., Akerstrom, U., Wulf, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Accumulation+of+sunscreen+in+human+skin+after+daily+applications:+a+study+of+sunscreens+with+different+ultraviolet+radiation+filters.">Accumulation of sunscreen in human skin after daily applications: a study of sunscreens with different ultraviolet radiation filters.</a> Photodermatol. Photoimmunol. Photomed. 28, 127-132 (2012).</li><li>Kwon, I. H., Kwon, H. H., Na, S. J., Youn, J. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Could+colorimetric+method+replace+the+individual+minimal+erythemal+dose+(MED)+measurements+in+determining+the+initial+dose+of+narrow-band+UVB+treatment+for+psoriasis+patients+with+skin+phototype+III-V?.">Could colorimetric method replace the individual minimal erythemal dose (MED) measurements in determining the initial dose of narrow-band UVB treatment for psoriasis patients with skin phototype III-V?.</a> J. Eur. Acad. Dermatol. Venereol. , (2012).</li><li>Youn, J. I., Park, J. Y., Jo, S. J., Rim, J. H., Choe, Y. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+the+usefulness+of+skin+phototype+and+skin+color+as+the+parameter+of+cutaneous+narrow+band+UVB+sensitivity+in+psoriasis+patients.">Assessment of the usefulness of skin phototype and skin color as the parameter of cutaneous narrow band UVB sensitivity in psoriasis patients.</a> Photodermatol. Photoimmunol. Photomed. 19, 261-264 (2003).</li><li>Henriksen, M., Na, R., Agren, M. S., Wulf, H. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Minimal+erythema+dose+after+multiple+UV+exposures+depends+on+pre-exposure+skin+pigmentation.">Minimal erythema dose after multiple UV exposures depends on pre-exposure skin pigmentation.</a> Photodermatol. Photoimmunol. Photomed. 20, 163-169 (2004).</li><li>Kraemer, C. K., Menegon, D. B., Cestari, T. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Determination+of+the+minimal+phototoxic+dose+and+colorimetry+in+psoralen+plus+ultraviolet+A+radiation+therapy.">Determination of the minimal phototoxic dose and colorimetry in psoralen plus ultraviolet A radiation therapy.</a> Photodermatol. Photoimmunol. Photomed. 21, 242-248 (2005).</li><li>Sachdeva, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fitzpatrick+skin+typing:+applications+in+dermatology.">Fitzpatrick skin typing: applications in dermatology.</a> Indian J. Dermatol. Venereol. Leprol. 75, 93-96 (2009).</li><li>Webb, A. R., Kift, R., Berry, J. L., Rhodes, L. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+vitamin+D+debate:+translating+controlled+experiments+into+reality+for+human+sun+exposure+times.">The vitamin D debate: translating controlled experiments into reality for human sun exposure times.</a> Photochem. Photobiol. 87, 741-745 (2011).</li><li>Otman, S. G., Edwards, C., Gambles, B., Anstey, A. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Validation+of+a+semiautomated+method+of+minimal+erythema+dose+testing+for+narrowband+ultraviolet+B+phototherapy.">Validation of a semiautomated method of minimal erythema dose testing for narrowband ultraviolet B phototherapy.</a> Br. J. Dermatol. 155, 416-421 (2006).</li><li>Taylor, D. K., Anstey, A. V., Coleman, A. J., Diffey, B. L., Farr, P. M., Ferguson, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guidelines+for+dosimetry+and+calibration+in+ultraviolet+radiation+therapy:+a+report+of+a+British+Photodermatology+Group+workshop.">Guidelines for dosimetry and calibration in ultraviolet radiation therapy: a report of a British Photodermatology Group workshop.</a> Br. J. Dermatol. 146, 755-763 (2002).</li></ol>

우리는 공개 아무것도 없어.

자외선 (UV)이 치료는 종종 건선, 여드름, 습진 등 다양한 일반적인 피부 조건에 대한 치료로 사용됩니다. UV 빛의 노출량은 개인의 피츠 패트릭 피부 타입의 함수로 결정되는 개인의 피부 민감도에 따라 처방되는 VI (매우 어두운 매우 공정)를 통해 I 2. 인간의 피부 때문에 변화의 UV 방사선 민감도에 따라 다릅니다 피부 착색, 간격, 및 기타 요인도. 따라서, 환자 관리하는 UV 빛의 적절한 복?...

minimal erythema dose (med) testing

자외선 (UV)이 치료는 종종 건선, 여드름, 습진 등 다양한 일반적인 피부 조건에 대한 치료로 사용됩니다. UV 빛의 복용량은 개인의 피부 민감도에 따라 규정된다. 따라서, 환자 관리하는 UV 빛의 적절한 복용량을 수립, 환자는 때때로 (울혈로 인해 햇볕이나 붉은 최소 홍반 생성됩니다 UV 방사선의 양을 최소 홍반 량 (MED)을 결정하기 위해 검사된다 모세 혈관의) 노출 후 몇 시간 내에 개인의 피부. 이 문서에서는 최소 홍반 량 (MED) 테스트를 수행하는 방법에 대해 설명합니다. 공식 MED 테스트를 실시하지 않고 임상 또는 연구 목적에 적합한 UV 량을 결정하는 쉬운 방법은 아래 또는 과다 복용의 위험과 관찰 테스트 후 시간 또는 비공식적 시행 착오 테스트를 필요로하는, 현재이 없습니다. 그러나 일부 다른 방법이 설명되어 있습니다.

<img alt="그림 1" fo:content-width="4in" fo:src="/files/ftp_upload/50175/50175fig1highres.jpg" src="/files/ftp_upload/50175/50175fig1.jpg" /> 그림 1 MED 테스트를 수행의 네 단계 :. MED를 평가, UV 노출을 실시하고, MED를 결정, UV 노출을 준비. <img alt="그림 2" src="/files/ftp_upload/50175/50175fig2.jpg" /><p class="jove_content" fo:keep-together...

Watch this Scientific Journal Video about Minimal Erythema Dose MED Testing at JoVE.com

Minimal Erythema Dose MED Testing

이 문서에서는 개인에게 투여시 홍반 (굽기) 원인이됩니다 자외선 방사선의 낮은 복용량을 결정하기 위해 최소 홍반 량 (MED) 테스트를 수행하는 방법에 대해 설명합니다.

최소 홍반 량 (MED) 시험

Ultraviolet radiation (UV) therapy is  sometimes used as a treatment for various common skin conditions, including  psoriasis, acne, and eczema. The ...

Universidad de Cartagena UDEC

Research

JoVE Journal

Medicine

Minimal Erythema Dose (MED) Testing

41.3K 조회수.  Fox Chase Cancer Center. 이 문서에서는 개인에게 투여시 홍반 (굽기) 원인이됩니다 자외선 방사선의 낮은 복용량을 결정하기 위해 최소 홍반 량 (MED) 테스트를 수행하는 방법에 대해 설명합니다.

비디오: Minimal Erythema Dose MED Testing

Quantitative Autonomic Testing

Disorders associated with dysfunction of autonomic nervous system are quite common yet frequently unrecognized. Quantitative autonomic testing can be invaluable tool for evaluation of these disorders, both in clinic and research. There are number of autonomic tests, however, only few were validated clinically or are quantitative. Here, fully quantitative and clinically validated protocol for testing of autonomic functions is presented. As a bare minimum the clinical autonomic laboratory should have a tilt table, ECG monitor, continuous noninvasive blood pressure monitor, respiratory monitor and a mean for evaluation of sudomotor domain. The software for recording and evaluation of autonomic tests is critical for correct evaluation of data. The presented protocol evaluates 3 major autonomic domains: cardiovagal, adrenergic and sudomotor. The tests include deep breathing, Valsalva maneuver, head-up tilt, and quantitative sudomotor axon test (QSART). The severity and distribution of dysautonomia is quantitated using Composite Autonomic Severity Scores (CASS). Detailed protocol is provided highlighting essential aspects of testing with emphasis on proper data acquisition, obtaining the relevant parameters and unbiased evaluation of autonomic signals. The normative data and CASS algorithm for interpretation of results are provided as well.

Standardized, comprehensive and fully quantitative testing of autonomic functions is described. The autonomic tests consist of evaluation of all three major autonomic domains including cardiovagal, adrenergic and sudomotor. The severity and distribution of dysautonomia is quantitated using Composite Autonomic Severity Scores.

양적 자율 테스트

Disorders associated with dysfunction of autonomic nervous system are quite common yet frequently unrecognized. Quantitative autonomic testing can be ...

연구

의학

X-ray Dose Reduction through Adaptive Exposure in Fluoroscopic Imaging

X-ray fluoroscopy is widely used for image guidance during cardiac intervention. However, radiation dose in these procedures can be high, and this is a significant concern, particularly in pediatric applications. Pediatrics procedures are in general much more complex than those performed on adults and thus are on average four to eight times longer1. Furthermore, children can undergo up to 10 fluoroscopic procedures by the age of 10, and have been shown to have a three-fold higher risk of developing fatal cancer throughout their life than the general population2,3.
We have shown that radiation dose can be significantly reduced in adult cardiac procedures by using our scanning beam digital x-ray (SBDX) system4-- a fluoroscopic imaging system that employs an inverse imaging geometry5,6 (Figure 1, Movie 1 and Figure 2). Instead of a single focal spot and an extended detector as used in conventional systems, our approach utilizes an extended X-ray source with multiple focal spots focused on a small detector. Our X-ray source consists of a scanning electron beam sequentially illuminating up to 9,000 focal spot positions. Each focal spot projects a small portion of the imaging volume onto the detector. In contrast to a conventional system where the final image is directly projected onto the detector, the SBDX uses a dedicated algorithm to reconstruct the final image from the 9,000 detector images.
For pediatric applications, dose savings with the SBDX system are expected to be smaller than in adult procedures. However, the SBDX system allows for additional dose savings by implementing an electronic adaptive exposure technique. Key to this method is the multi-beam scanning technique of the SBDX system: rather than exposing every part of the image with the same radiation dose, we can dynamically vary the exposure depending on the opacity of the region exposed. Therefore, we can significantly reduce exposure in radiolucent areas and maintain exposure in more opaque regions. In our current implementation, the adaptive exposure requires user interaction (Figure 3). However, in the future, the adaptive exposure will be real time and fully automatic.
We have performed experiments with an anthropomorphic phantom and compared measured radiation dose with and without adaptive exposure using a dose area product (DAP) meter. In the experiment presented here, we find a dose reduction of 30%.

We are developing a dynamic adaptive exposure technique using our scanning beam digital X-ray system. Rather than exposing an object uniformly, the exposure is adapted depending on the opacity of the object. Here we show an experiment on an anthropomorphic phantom that resulted in a dose saving of 30%.

Fluoroscopic 영상에 적응 노출을 통해 X - 선 선량 감소

X-ray fluoroscopy is widely used for image guidance during cardiac intervention. However, radiation dose in these procedures can be high, and this is a ...

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the highest possible level of safety for transfusion patients, including protection from transfusion transmitted diseases. This must be accomplished in a fiscally responsible manner which minimizes operating expenses including consumables, equipment, waste, and personnel costs, among others.
Several methods are available to produce platelet concentrates for transfusion. One of the most common is the buffy coat method in which a single therapeutic platelet unit (&ge; 2.0 x1011 platelets per unit or per local regulations) is prepared by pooling the buffy coat layer from up to six whole blood donations. A procedure for producing "double dose" whole blood derived platelets has only recently been developed.
Presented here is a novel method for preparing double dose whole blood derived platelet concentrates from pools of 7 buffy coats and subsequently treating the double dose units with the INTERCEPT Blood System for pathogen inactivation. INTERCEPT was developed to inactivate viruses, bacteria, parasites, and contaminating donor white cells which may be present in donated blood. Pairing INTERCEPT with the double dose buffy coat method by utilizing the INTERCEPT Processing Set with Dual Storage Containers (the "DS set"), allows blood centers to treat each of their double dose units in a single pathogen inactivation processing set, thereby maximizing patient safety while minimizing costs. The double dose buffy coat method requires fewer buffy coats and reduces the use of consumables by up to 50% (e.g. pooling sets, filter sets, platelet additive solution, and sterile connection wafers) compared to preparation and treatment of single dose buffy coat platelet units. Other cost savings include less waste, less equipment maintenance, lower power requirements, reduced personnel time, and lower collection cost compared to the apheresis technique.

This article describes the process used by &#214;rebro University Hospital to produce double dose buffy coat platelet concentrates prepared from whole blood donations and treated with the INTERCEPT Blood System for pathogen inactivation. The in vitro quality of the final platelet units are evaluated over 7 days of storage.

차단 혈액 시스템을 사용하여 준비 및 더블 복용 버피 코트의 혈소판 제품의 병원체 불 활성화

Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the ...

A Novel Application of Musculoskeletal Ultrasound Imaging

Ultrasound is an attractive modality for imaging muscle and tendon motion during dynamic tasks and can provide a complementary methodological approach for biomechanical studies in a clinical or laboratory setting. Towards this goal, methods for quantification of muscle kinematics from ultrasound imagery are being developed based on image processing. The temporal resolution of these methods is typically not sufficient for highly dynamic tasks, such as drop-landing. We propose a new approach that utilizes a Doppler method for quantifying muscle kinematics. We have developed a novel vector tissue Doppler imaging (vTDI) technique that can be used to measure musculoskeletal contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities using ultrasound. The goal of this preliminary study was to investigate the repeatability and potential applicability of the vTDI technique in measuring musculoskeletal velocities during a drop-landing task, in healthy subjects. The vTDI measurements can be performed concurrently with other biomechanical techniques, such as 3D motion capture for joint kinematics and kinetics, electromyography for timing of muscle activation and force plates for ground reaction force. Integration of these complementary techniques could lead to a better understanding of dynamic muscle function and dysfunction underlying the pathogenesis and pathophysiology of musculoskeletal disorders.

We describe a new ultrasound-based vector tissue Doppler imaging technique to measure muscle contraction velocity, strain and strain rate with sub-millisecond temporal resolution during dynamic activities. This approach provides complementary measurements of dynamic muscle function and could lead to a better understanding of mechanisms underlying musculoskeletal disorders.

근골격계 초음파 이미징의 소설 응용 프로그램

Ultrasound is an attractive modality for imaging muscle and tendon  motion during dynamic tasks and can provide a complementary methodological  approach ...

Voluntary Breath-hold Technique for Reducing Heart Dose in Left Breast Radiotherapy

Breath-holding techniques reduce the amount of radiation received by cardiac structures during tangential-field left breast radiotherapy. With these techniques, patients hold their breath while radiotherapy is delivered, pushing the heart down and away from the radiotherapy field. Despite clear dosimetric benefits, these techniques are not yet in widespread use. One reason for this is that commercially available solutions require specialist equipment, necessitating not only significant capital investment, but often also incurring ongoing costs such as a need for daily disposable mouthpieces. The voluntary breath-hold technique described here does not require any additional specialist equipment. All breath-holding techniques require a surrogate to monitor breath-hold consistency and whether breath-hold is maintained. Voluntary breath-hold uses the distance moved by the anterior and lateral reference marks (tattoos) away from the treatment room lasers in breath-hold to monitor consistency at CT-planning and treatment setup. Light fields are then used to monitor breath-hold consistency prior to and during radiotherapy delivery.

The current priority in breast cancer radiotherapy is to reduce cardiac doses without compromising target tissue coverage. The voluntary breath-hold technique described here is a simple, inexpensive solution to this problem and capable of being instituted widely without the need for specialized equipment.

왼쪽 유방 방사선 치료에 심장 선량 감소를위한 자발적 호흡 홀드 기술

Breath-holding techniques reduce the amount of radiation received by cardiac structures during tangential-field left breast radiotherapy. With these ...

Minimal Invasive Surgical Procedure of Inducing Myocardial Infarction in Mice

Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last decades. For clarification of the underlying mechanisms and the development of new therapeutic strategies, the availability of valid animal models are mandatory. Since we need new insights into pathomechanisms of cardiovascular diseases under in vivo conditions to combat myocardial infarction, the validity of the animal model is a crucial aspect. However, protection of animals are highly relevant in this context. Therefore, we establish a minimally invasive and simple model of myocardial infarction in mice, which assures a high reproducibility and survival rate of animals. Thus, this models fulfils the requirements of the 3R principle (Replacement, Refinement and Reduction) for animal experiments and assure the scientific information needed for further developing of therapeutical strategies for cardiovascular diseases.

A highly reproducible model for myocardial infarction in mice with minimal invasive manipulations is described. The model can be easily performed, resulting in a high reproducibility and survival rate. Thus, the described model will reduce the number of required animals as requested by the 3R principle (Replacement, Refinement and Reduction).

쥐 심근 경색을 유도 최소 침습 수술

Myocardial infarction still remains the main cause of death in western countries, despite considerable progress in the stent development area in the last ...

Intracoronary Acetylcholine Provocation Testing for Assessment of Coronary Vasomotor Disorders

Intracoronary acetylcholine provocation testing (ACH-test) is an established method for assessment of epicardial coronary artery spasm in the catheterization laboratory which was introduced more than 30 years ago. Due to the short half-life of acetylcholine it can only be applied directly into the coronary arteries. Several studies have demonstrated the safety and clinical usefulness of this test. However, acetylcholine testing is only rarely applied in the U.S. or Europe. Nevertheless, it has been shown that 62% of Caucasian patients with stable angina and unobstructed coronary arteries on coronary angiography suffer from coronary vasomotor disorders that can be diagnosed with acetylcholine testing. In recent years it has been appreciated that the ACH-test not only assesses the presence of epicardial spasm but that it can also be useful for the detection of coronary microvascular spam. In such cases no epicardial spasm is seen after injection of acetylcholine but ischemic ECG shifts are present together with a reproduction of the patient's symptoms during the test. This article describes the experience with the ACH-test and its implementation in daily clinical routine.

Intracoronary acetylcholine testing has been established for the assessment of epicardial coronary spasm more than 30 years ago. Recently, the focus has shifted towards the microcirculation and it has been shown that microvascular spasm can be detected using ACH-testing. This article describes the ACH-test and its implementation in daily routine.

평가 관상 동맥의 혈관 운동 장애에 대한 관상 동맥 내 아세틸 콜린 도발 테스트

Intracoronary acetylcholine provocation testing (ACH-test) is an established method for assessment of epicardial coronary artery spasm in the ...

Technique and Patient Selection Criteria of Right Anterior Mini-Thoracotomy for Minimal Access Aortic Valve Replacement

Aortic valve stenosis has become the most prevalent valvular heart disease in developed countries, and is due to the aging of these populations. The incidence of the pathology increases with growing age after 65 years. Conventional surgical aortic valve replacement through median sternotomy has been the gold standard of patient care for symptomatic aortic valve stenosis. However, as the risk profile of patients worsens, other therapeutic strategies have been introduced in an attempt to maintain the excellent results obtained by the established surgical treatment. One of these approaches is represented by transcatheter aortic valve implantation. Although the outcomes of high-risk patients undergoing treatment for symptomatic aortic valve stenosis have improved with transcatheter aortic valve replacement, many patients with this condition remain candidates for surgical aortic valve replacement. In order to reduce the surgical trauma in patients who are candidates for surgical aortic valve replacement, minimally invasive approaches have garnered interest during the past decade. Since the introduction of right anterior thoracotomy for aortic valve replacement in 1993, right anterior mini-thoracotomy and upper hemi-sternotomy have become the predominant incisional approaches among cardiac surgeons performing minimal access aortic valve replacement. Beside the location of the incision, the arterial cannulation site represents the second major landmark of minimal access techniques for aortic valve replacement. The two most frequently used arterial cannulation sites include central aortic and peripheral femoral approaches. With the purpose of reducing surgical trauma in these patients, we have opted for a right anterior mini-thoracotomy approach with a central aortic cannulation site. This protocol describes in detail a technique for minimally invasive aortic valve replacement and provides recommendations for patient selection criteria, including cardiac computer tomography measurements. The indications and limitations of this technique, as well as its alternatives, are discussed.

The goal of this protocol is to describe in detail the technique of minimally invasive aortic valve replacement through a right anterior mini-thoracotomy and central aortic cannulation. This technique can potentially enhance patients&#39; comfort and, by reducing post-operative morbidity, promote lowering the length of stay and global costs.

기술 및 최소한의 액세스 대동맥 밸브 교체에 대 한 바로 앞쪽 미니 Thoracotomy의 환자 선택 기준

Aortic valve stenosis has become the most prevalent valvular heart disease in developed countries, and is due to the aging of these populations. The ...

Ultrasonography of the Adult Male Urinary Tract for Urinary Functional Testing

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting in a significant economic and quality of life burden. Transgenic and other mouse models have been developed to recreate various aspects of this multifactorial disease; however, methods to accurately quantitate urinary dysfunction and the effectiveness of new therapeutic options are lacking. Here, we describe a method that can be used to measure bladder volume and detrusor wall thickness, urinary velocity, void volume and void duration, and urethral diameter. This would allow for the evaluation of disease progression and treatment efficacy over time. Mice were anesthetized with isoflurane, and the bladder was visualized by ultrasound. For non-contrast imaging, a 3D image was taken of the bladder to calculate volume and evaluate shape; the bladder wall thickness was measured from this image. For contrast-enhanced imaging, a catheter was placed through the dome of the bladder using a 27-gauge needle connected to a syringe by PE50 tubing. A bolus of 0.5 mL of contrast was infused into the bladder until a urination event occurred. Urethral diameter was determined at the point of the Doppler velocity sample window during the first voiding event. Velocity was measured for each subsequent event yielding a flow rate. In conclusion, high frequency ultrasound proved to be an effective method for assessing bladder and urethral measurements during urinary function in mice. This technique may be useful in the assessment of novel therapies for BPH/LUTS in an experimental setting.

We describe the use of high frequency ultrasound with contrast imaging as a method to measure bladder volume, bladder wall thickness, urine velocity, void volume, void duration, and urethral diameter. This strategy can be used to assess voiding dysfunction and treatment efficacy in various mouse models of lower urinary tract dysfunction (LUTD).

요로 기능 검사를 위한 성인 남성 요로의 초음파 검사

The incidence of clinical benign prostatic hyperplasia (BPH) and lower urinary tract symptoms (LUTS) is increasing due to the aging population, resulting ...

Precision Implementation of Minimal Erythema Dose (MED) Testing to Assess Individual Variation in Human Inflammatory Response

Minimal erythema dose (MED) testing is frequently used in clinical settings for determining the smallest amount of ultraviolet (UV) irradiation necessary to produce erythema (inflammatory reddening) on the surface of the skin. In this context, the MED is regarded as a key factor in determining starting doses for UV phototherapy for common skin conditions such as psoriasis and eczema. In research settings, MED testing also has potential to be a powerful tool for assessing within- and between-persons variation in inflammatory responses. However, MED testing has not been widely adopted for use in research settings, likely owing to a lack of published guidelines, which is a barrier to obtaining reproducible results from this assay. Also, protocols and equipment for establishing MED vary widely, making it difficult to compare results across laboratories. Here, we describe a precise and reproducible method to induce and measure superficial erythema using newly designed protocols and methods that can easily be adapted to other equipment and laboratory environments. The method described here includes detail on procedures that will allow extrapolation of a standardized dosage schedule to other equipment so that this protocol can be adapted to any UV radiation source.

Precision Implementation of Minimal Erythema Dose MED Testing to Assess Individual Variation in Human Inflammatory Response

Minimal erythema dose (MED) testing is used to establish dosage schedules for ultraviolet radiation phototherapy. It can assess individual variation in inflammatory response but lacks methodology for achieving reproducible results. Here, we present a precision implementation of MED and demonstrate its ability to capture individual variation in inflammatory response.

최소한의 홍반 복용량의 정밀 한 구현 (MED) 인간의 염증 반응에 개별 변화를 평가 하는 테스트

Minimal erythema dose (MED) testing is frequently used in clinical settings for determining the smallest amount of ultraviolet (UV) irradiation necessary ...