ホスホロチオエートを使用して RNA の結合蛋白質の新規飽和突然変異誘発方法: シングル ステップの特性

0:30

Generation of a Library of Mutants - Doping DNA Template with Non-wild Type Nucleotides

1:20

Synthesis of RNA

5:24

Protein Binding Reaction and Separation of Bound RNA

8:05

Analysis of Iodine-cleaved Phosphorothioate Products for Detection of Mutant Nucleotide Positions

9:07

Results: Characterization of Protein Binding Site in RNA

10:22

Conclusion