作者は彼らの役に立つ議論や技術支援のための産婦人科のメンバーと癌生物学に感謝します。この研究は、科学の振興（JSPS）費補助金科学研究費（; H.梶山へ15K15604）のために日本協会によってサポートされていました。

治療プロトコルは名古屋大学で採択された動物実験のためのガイドラインに従います。 細胞懸濁液の調製<ol><li>ヒト卵巣明細胞癌細胞株。 <ol><li> （100単位/ ml、ストレプトマイシン：0.1mg / mlのペニシリン）、10％ウシ胎児血清（FBS）及びペニシリン/ストレプトマイシンを含むRPMI-1640培地中でES-2細胞12を保持します。 5％CO 2の加湿インキュベーター中で37℃で培養細胞。 </li><li> 0.05％トリプシン/ 0.02％EDTA溶液（トリプシン/ EDTA）で対数増殖期の細胞を収集します。 <ol><li> （ - 25cm 2のあたり4ミリリットル、2+ の Ca 2+ / Mgを含まないPBS 3）まず、次いでPBSで細胞を1回洗浄し、細胞培養容器から古いメディアを削除します。 </li><li>次に、トリプシン/ EDTAの25cm 2のあたり1ミリリットルを追加します。トリプシン/ EDTAで細胞単層をカバーするために細胞培養容器を回転させます。トリプシン/ EDTAを除去し、廃棄した後、細胞を返します5分または細胞が分離されるまで - 3のためのインキュベーターに培養容器。 </li><li>細胞は、細胞培養容器の表面から剥離して浮いていることを確認するために顕微鏡下で細胞を調べます。 </li><li>トリプシン（25cm 2のあたり5 ml）を不活性化し、細胞を再懸濁するために、増殖培地を含有する新鮮な血清を加えます。 15ミリリットルコニカルチューブに再懸濁した細胞を転送します。 </li><li> 5分間、300×gで遠心分離します。 4℃または室温での遠心分離のいずれかを実行します。慎重に上清を除去し、（25cm 2のあたり5 ml）を成長培地で細胞を再懸濁します。 </li></ol></li><li> （トリパンブルー排除によって、例えば ）細胞カウントを2×10 8細胞/ mlの密度で増殖培地中に再懸濁します。氷の上に懸濁した細胞を置きます。 </li></ol></li><li>凍結した細胞外マトリックス（ECM）を解凍氷浴中でヒドロゲルアリコートをベース。 </li><li> 1（最後のセルC：1の比率でECMベースのヒドロゲルのアリコートに細胞懸濁液を追加oncentration：1×10 5細胞/培地-ECMベースのヒドロゲルのμl）を、徹底的に混ぜます。使用するまで氷浴中で調製した細胞懸濁液を置きます。 </li></ol>細胞懸濁液2.同所接種注：手術は、専用のスイートを必要としません。手術は別個であり、最小限の活性を有する部屋の領域で実験室のベンチトップ上で実行することができます。層流フードを使用してもよいです。無菌の手術器具を使用する必要があり、鋏は、皮膚切開を作製するために使用すべきではありません。 <ol><li>この同所接種モデルのための8週齢で雌性ヌードマウス（BALB / C NU / NU）を使用します。 </li><li> （ - 4％2）まず、イソフルラン吸入を用いてマウスを麻酔。しっかりとつま先のピンチの際に撤退の欠如を検証することによって麻酔深度を確認してください。マウスを麻酔した後、必要に応じて乾燥を防ぐために、目に当たり障りのない無菌眼軟膏を適用します。 </li><li> operatに麻酔マウスを置きます段腹側を下る。アルコールとヨウ素系またはクロルヘキシジンベースのスクラブの両方で手術領域を数回駆除。 </li><li>垂直方向に右肋骨弓と大腿骨の間の背骨に近い〜2 cmの切開を行います。切開部位の周りに滅菌外科用ドレープを使用してください。開口部を維持するために、クランプで切開し、皮膚の表面を修正しました。 注：リトラクターは、代わりのクランプを使用することができます。 </li><li>次に、腹腔を開くには、最初の切開下壁側腹膜で第2の切開（約1cm）を作ります。切開腹部の表面を固定します。腹腔から卵巣を除去するために、クランプで同側卵巣と卵管を囲む脂肪組織をクリップ。慎重にガーゼ上の卵巣と卵管を配置します。その後、解剖顕微鏡下でマウスの腹側を下に置きます。 </li><li>注入直前（1/2 ccで、29 G）は、インスリン注射器で細胞懸濁液を置きます。慎重に準備された細胞懸濁液を（注入1- 卵巣に2μl）を。いくつかの秒間、ECMベースのハイドロゲルのゲル化を確実にするために卵巣内針を保持します。ゲル化した後、注入された腫瘍細胞が卵巣の内部に残っています。 </li><li>慎重に体内に卵巣を返します。滅菌医療絹を有する第二の切開を縫合し、次に創傷クリップで皮膚を密封します。 </li><li>手術後、マウスは、最初の24時間の期間（ 例えば 、メロキシカム、筋肉内（IM）又は皮下（SC）、0.2ミリグラム/ kg）の鎮痛を投与されます。 </li></ol> 3.手術後の処置<ol><li>それは胸骨横臥位を維持するのに十分な意識を取り戻したまでマウスを暖かく保つためにホットパックできれいに紙の上に別々のケージに各マウスを置きます。その後、飼育および監視するためのケージに動物を返します。外科的治療後の7日 - 3毎日マウスを監視します。 </li><li>術後のマウスのモニタリング<ol><li>痛みの兆候の場合には、analgeを投与毎日SICS（ 例えば 、メロキシカム、筋肉内または皮下、0.2ミリグラム/キログラム）。 </li><li>マウスは感染症（ 例えば 、発赤、腫脹、放電）の兆候がある場合は、抗感染剤（ 例えば 、ペニシリン、イム10万IU / kg）を毎日注射を投与します。 </li></ol></li><li> 14日手術後の治療後 - 10皮膚閉鎖材料を削除します。 </li></ol>腫瘍および転移の4分析注： - 接種後3週間で腫瘍が注入された卵巣2に形成されます。 <ol><li>さらなる分析14,16-20のために（ 例えば 、腫瘍、転移、臓器）13-15二酸化炭素によりマウスを犠牲にしてから、サンプルを収集。 注： インビボイメージングシステムは、利用可能である場合には生物発光（ 例えば 、ルシフェラーゼ）、または蛍光タンパク質（ 例えば 、GFP）を産生する酵素を有する細胞は、このプロトコルで使用することができます。この修正されたプロトコルは、有用な情報を提供します原発腫瘍からの癌細胞の広がりのダイナミクス。 </li></ol>

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著者らは、開示することは何もありません。

動物モデルは、癌細胞に対する腫瘍発生、進行、転移、及び薬効のメカニズムを分析するために不可欠です。ヒト卵巣癌細胞を有するマウスはまた、動物モデルとして使用されてきました。ここでは、同所のサイトで卵巣腫瘍細胞の投与のための低侵襲性の手順を説明します。この手順を使用して、背側脇腹から後腹膜アプローチを通じて卵巣への接種は、他の一般的に使用される技術上の?...

上皮性卵巣癌（EOC）は、婦人科悪性腫瘍の1の中で癌関連死亡率の最も高い割合を占めています。 EOCは、主にその後半症状に、予後不良と関連している、と多くの場合、複数の腹腔内disseminationsおよび遠隔転移2-4と関連しています。腹膜播種は、多段階プロセスです。まず、腫瘍細胞は原発巣から離れ、そして腹腔内に移動します。腫瘍細胞が腹膜中皮に付着すると、彼らは中皮5,6を介して組織に侵入し始めます。より良い腫瘍生物学（ 例えば 、癌の進行と治療反応）を理解するために、マウスモデルは、豊富な情報を提供しています。ヒト癌細胞を有する異種移植片は、広く、静脈内に、腹腔内に、ヒト癌細胞を皮下に接種されたマウスモデルに使用され、同所れます。同所gを有する動物モデルrafted腫瘍は、より効率的かつ正確heterotopicallyグラフトされた腫瘍を有する動物モデルと比較して、ヒトにおける腫瘍環境を反映した結果を生成することができます。そのため、多くのヒト腫瘍については、同所移植モデルは7月11日に設立されました。 ここでは、腹側接種に比べて侵襲性が制限されている背側脇腹から後腹膜アプローチを通じて人EOC細胞の同所接種を説明します。この技術はEOCについての有用な情報、腹腔内播種の特にメカニズムの多様性を提供することができます。

<table><tbody><tr><td>Matrigel matrix&amp;nbsp;</td><td>BD</td><td>354234</td><td>ECM-based hydrogel</td></tr><tr><td>Insulin syringe</td><td>TERUMO</td><td>SS-05M2913</td><td>1/2 cc, 29 G x 1/2&quot;</td></tr><tr><td>Suturing needle with suture</td><td></td><td></td><td>11 mm, 3/8, Nylon6-0</td></tr><tr><td>Reflex 7 mm Wound Clip Applier</td><td>CellPoint Scientific</td><td>204-1000</td><td></td></tr><tr><td>&amp;nbsp;Reflex 7 mm wound clips</td><td>CellPoint Scientific</td><td>203-1000</td><td></td></tr></tbody></table>

murine experimental model of original tumor development and peritoneal metastasis via orthotopic inoculation with ovarian carcinoma cells

それは腹膜播種を示しているので、上皮性卵巣癌（EOC）は予後不良と関連しています。予後を改善するために、腹膜播種を制御することが重要です。しかし、腫瘍細胞が原発巣から切り離し、中皮に付着するかまだ不明です。適切な動物モデルの確立は、in vivoでの腹膜播種のメカニズムの理解を得るために必要とされます。現在の研究では、腹膜や遠隔臓器などの転移の発生にマウスの卵巣表面にEOC細胞の局所注射からプロセスをご紹介します。生後8週目の雌ヌードマウス（BALB / C NU / NU）を使用しました。ビューの顕微鏡視野下では、EOC細胞（中の細胞外マトリックス（ECM）の1×10 5細胞/μlのヒドロゲル/片側性卵巣/マウスをベース）背側脇腹から後腹膜アプローチにより、マウスの卵巣に注入しました。この提案された方法は、ルですマウスのためのssの侵襲的手技とは、卵巣へのダメージを最小限に抑えることができます。ここでは、EOCの元と転移性腫瘍形成の開発における方法論の手順を説明します。

<html>六ヌードマウスは、このプロトコルに記載のように、それらの卵巣は、ヒト明細胞癌細胞株ES-2を接種していました。 図1Aに示すように、2週間後、6匹のマウスの5は、ES-2細胞を接種した卵巣の腫瘍を有していたが、反対側の卵巣には腫瘍（コントロールとして使用）を接種せずに存在しませんでした。また、5匹のマウスの2は、腹水を持つ複数の腹膜d...

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Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

卵巣癌の腹膜播種の多段階プロセスを明確にするために、現在の研究では、これらの腫瘍細胞と同所接種を介して元の腫瘍の開発と腹膜転移のマウス実験モデルを提示します。

オリジナル腫瘍の開発のマウス実験モデルと腹膜転移<em&gt;経由で</em卵巣癌細胞で&gt;同所接種

Epithelial ovarian carcinoma (EOC) is associated with a poor prognosis because it shows peritoneal dissemination. To improve the prognosis, it is ...

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Universidad de Cartagena UDEC

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Cancer Research

Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

10.4K ビュー.  Bell Research Center for Reproductive Health and Cancer. 卵巣癌の腹膜播種の多段階プロセスを明確にするために、現在の研究では、これらの腫瘍細胞と同所接種を介して元の腫瘍の開発と腹膜転移のマウス実験モデルを提示します。 

ビデオ: Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

Background: Ovarian cancer is generally diagnosed at an advanced stage where the case/fatality ratio is high and thus remains the most lethal of all gynecologic malignancies among US women 1,2,3. Serous tumors are the most widespread forms of ovarian cancer and 4,5 the Tg-MISIIR-TAg transgenic represents the only mouse model that spontaneously develops this type of tumors. Tg-MISIIR-TAg mice express SV40 transforming region under control of the Mullerian Inhibitory Substance type II Receptor (MISIIR) gene promoter 6. Additional transgenic lines have been identified that express the SV40 TAg transgene, but do not develop ovarian tumors. Non-tumor prone mice exhibit typical lifespan for C57Bl/6 mice and are fertile. These mice can be used as syngeneic allograft recipients for tumor cells isolated from Tg-MISIIR-TAg-DR26 mice.
Objective: Although tumor imaging is possible 7, early detection of deep tumors is challenging in small living animals. To enable preclinical studies in an immunologically intact animal model for serous ovarian cancer, we describe a syngeneic mouse model for this type of ovarian cancer that permits in vivo imaging, studies of the tumor microenvironment and tumor immune responses.
Methods: We first derived a TAg+ mouse cancer cell line (MOV1) from a spontaneous ovarian tumor harvested in a 26 week-old DR26 Tg-MISIIR-TAg female. Then, we stably transduced MOV1 cells with TurboFP635 Lentivirus mammalian vector that encodes Katushka, a far-red mutant of the red fluorescent protein from sea anemone Entacmaea quadricolor with excitation/emission maxima at 588/635 nm 8,9,10. We orthotopically implanted MOV1Kat in the ovary 11,12,13,14 of non-tumor prone Tg-MISIIR-TAg female mice. Tumor progression was followed by in vivo optical imaging and tumor microenvironment was analyzed by immunohistochemistry.
Results: Orthotopically implanted MOV1Kat cells developed serous ovarian tumors. MOV1Kat tumors could be visualized by in vivo imaging up to three weeks after implantation (fig. 1) and were infiltrated with leukocytes, as observed in human ovarian cancers 15 (fig. 2).
Conclusions: We describe an orthotopic model of ovarian cancer suitable for in vivo imaging of early tumors due to the high pH-stability and photostability of Katushka in deep tissues. We propose the use of this novel syngeneic model of serous ovarian cancer for in vivo imaging studies and monitoring of tumor immune responses and immunotherapies.

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses

To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.

のための免疫担当マウスにおける漿液性卵巣癌の同所性モデル in vivoで</em腫瘍免疫応答の>腫瘍イメージングとモニタリング

Background: Ovarian cancer is generally diagnosed at an advanced stage where the case/fatality ratio is high and thus remains the most lethal of all ...

免疫・感染学

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

Ovarian cancer is characterized by early, diffuse metastasis with 70% of women having metastatic disease at the time of diagnosis. While elegant transgenic mouse models of ovarian cancer exist, these mice are expensive and take a long time to develop tumors. Intraperitoneal injection xenograft models lack human stroma and do not accurately model ovarian cancer metastasis. Even patient derived xenografts (PDX) do not fully recapitulate the human stromal microenvironment as serial PDX passages demonstrate significant loss of human stroma. The ability to easily model human ovarian cancer within a physiologically relevant stromal microenvironment is an unmet need. Here, the protocol presents an orthotopic ovarian cancer mouse model using human ovarian cancer cells combined with patient-derived carcinoma-associated mesenchymal stem cells (CA-MSCs). CA-MSCs are stromal progenitor cells, which drive the formation of the stromal microenvironment and support ovarian cancer growth and metastasis. This model develops early and diffuses metastasis mimicking clinical presentation. In this model, luciferase expressing ovarian cancer cells are mixed in a 1:1 ratio with CA-MSCs and injected into the ovarian bursa of NSG mice. Tumor growth and metastasis are followed serially over time using bioluminescence imaging. The resulting tumors grow aggressively and form abdominal metastases by 14 days post injection. Mice experienced significant decreases in body weight as a marker of systemic illness and increased disease burden. By day 30 post injection, mice met endpoint criteria of &gt;10% body weight loss and necropsy confirmed intra-abdominal metastasis in 100% of mice and 60%-80% lung and parenchymal liver metastasis. Collectively, orthotopic engraftment of ovarian cancer cells and stroma cells generates tumors that closely mimic the early and diffuse metastatic behavior of human ovarian cancer. Furthermore, this model provides a tool to study the role of ovarian cancer cell: stroma cell interactions in metastatic progression.

Orthotopic engraftment of ovarian cancer cells mixed with human stromal cells provides a mouse model that exhibits rapid, diffuse metastatic behavior that is characteristic of human ovarian cancer. This model also allows for the study of tumor cell and stromal cell interactions, as well as their role in tumor progression and metastasis.

Ovarian cancer is characterized by early, diffuse metastasis with 70% of women having metastatic disease at the time of diagnosis. While elegant ...

がん研究

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Osteosarcoma is the most common primary bone cancer in children and adolescents, with lungs as the most common metastatic site. The five-year survival rate of osteosarcoma patients with pulmonary metastasis is less than 30%.Therefore, the utilization of mouse models mimicking the osteosarcoma development in humans is of great significance for understanding the fundamental mechanism of osteosarcoma carcinogenesis and pulmonary metastasis to develop novel therapeutics. Here, detailed procedures are reported to generate the primary osteosarcoma and pulmonary metastasis mouse models via&#160;intratibia injection of osteosarcoma cells. Combined with the bioluminescence or X-ray live imaging system, these living mouse models are utilized to monitor and quantify osteosarcoma growth and metastasis. To establish this model, a basement membrane matrix containing osteosarcoma cells was loaded in a micro-volume syringe and injected into one tibia of each athymic mouse after being anesthetized. The mice were sacrificed when the primary osteosarcoma reached the size limitation in the IACUC-approved protocol. The legs bearing osteosarcoma and the lungs with metastasis lesions were separated. These models are characterized by a short incubation period, rapid growth, severe lesions, and sensitivity in monitoring the development of primary and pulmonary metastatic lesions. Therefore, these are ideal models for exploring the functions and mechanisms of specific factors in osteosarcoma carcinogenesis and pulmonary metastasis, the tumor microenvironment, and evaluating the therapeutic efficacy in vivo.

The present protocol describes intratibia osteosarcoma cell injection to generate mouse models bearing orthotopic osteosarcoma and pulmonary metastasis lesions.

同所性骨肉腫および肺転移マウスモデルを生成するための脛骨内骨肉腫細胞注射

Osteosarcoma is the most common primary bone cancer in children and adolescents, with lungs as the most common metastatic site. The five-year survival ...

Murine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of care consisting of surgical debulking and combination chemotherapy consisting of platinum and taxane compounds, almost 90% of patients recur within a few years. In these patients the development of chemoresistant disease limits the efficacy of currently available chemotherapy agents and therefore contributes to the high mortality. To discover novel therapy options that can target recurrent disease, appropriate animal models that closely mimic the clinical profile of patients with recurrent ovarian cancer are required. The challenge in monitoring intra-peritoneal (i.p.) disease limits the use of i.p. models and thus most xenografts are established subcutaneously. We have developed a sensitive optical imaging platform that allows the detection and anatomical location of i.p. tumor mass. The platform includes the use of optical reporters that extend from the visible light range to near infrared, which in combination with 2-dimensional X-ray co-registration can provide anatomical location of molecular signals. Detection is significantly improved by the use of a rotation system that drives the animal to multiple angular positions for 360 degree imaging, allowing the identification of tumors that are not visible in single orientation. This platform provides a unique model to non-invasively monitor tumor growth and evaluate the efficacy of new therapies for the prevention or treatment of recurrent ovarian cancer.

We describe a non-invasive animal imaging platform that allows the detection, quantification, and monitoring of ovarian cancer growth and recurrence. This intra-peritoneal xenograft model mimics the clinical profile of patients with ovarian cancer.

卵巣がん再発を検出し、モニターするための非侵襲的イメージングのためのマウスモデル

Epithelial ovarian cancer is the most lethal gynecologic malignancy in the United States. Although patients initially respond to the current standard of ...

医学

卵巣がん-大網相互作用のための3D ex vivoモデルの確立

An Ex Vivo Model of Ovarian Cancer Peritoneal Metastasis Using Human Omentum

Ovarian cancer is the deadliest gynecologic malignancy. The omentum plays a key role in providing a supportive microenvironment to metastatic ovarian cancer cells as well as immune modulatory signals that allow tumor tolerance. However, we have limited models that closely mimic the interaction between ovarian cancer cells and adipose-rich tissues. To further understand the cellular and molecular mechanisms by which the omentum provides a pro-tumoral microenvironment, we developed a unique 3D ex vivo model of cancer cell-omentum interaction. Using human omentum, we are able to grow ovarian cancer cells within this adipose-rich microenvironment and monitor the factors responsible for tumor growth and immune regulation. In addition to providing a platform for the study of this adipose-rich tumor microenvironment, the model provides an excellent platform for the development and evaluation of novel therapeutic approaches to target metastatic cancer cells in this niche. The proposed model is easy to generate, inexpensive, and applicable to translational investigations.

著者スポットライト:がんと脂肪微小環境の相互作用を調査するための高度なex vivoモデル 

This protocol describes the establishment of a three-dimensional (3D) ex vivo model of cancer cell-omentum interaction. The model provides a platform for elucidating pro-tumor mechanisms within the adipose niche and for testing novel therapies.

ヒト大網を用いた卵巣癌腹膜転移の Ex vivo モデル

Ovarian cancer is the deadliest gynecologic malignancy. The omentum plays a key role in providing a supportive microenvironment to metastatic ovarian ...

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Human cancer and response to therapy is better represented in orthotopic animal models. This paper describes the development of an orthotopic mouse model of ovarian cancer, treatment of cancer via oral delivery of drugs, and monitoring of tumor cell behavior in response to drug treatment in real time using in vivo imaging system. In this orthotopic model, ovarian tumor cells expressing luciferase are applied topically by injecting them directly into the mouse bursa where each ovary is enclosed. Upon injection of D-luciferin, a substrate of firefly luciferase, luciferase-expressing cells generate bioluminescence signals. This signal is detected by the in vivo imaging system and allows for a non-invasive means of monitoring tumor growth, distribution, and regression in individual animals. Drug administration via oral gavage allows for a maximum dosing volume of 10 mL/kg body weight to be delivered directly to the stomach and closely resembles delivery of drugs in clinical treatments. Therefore, techniques described here, development of an orthotopic mouse model of ovarian cancer, oral delivery of drugs, and in vivo imaging, are useful for better understanding of human ovarian cancer and treatment and will improve targeting this disease.

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer

Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.

生体イメージングおよび卵巣癌の同所性マウスモデルにおける治療的処置の

Human cancer and response to therapy is better represented in orthotopic animal models.  This paper describes the development of an orthotopic mouse model ...

生物学

Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy in the United States. Mortality is due to diagnosis of 75% of women with late stage disease, when metastasis is already present. EOC is characterized by diffuse and widely disseminated intra-peritoneal metastasis. Cells shed from the primary tumor anchor in the mesothelium that lines the peritoneal cavity as well as in the omentum, resulting in multi-focal metastasis, often in the presence of peritoneal ascites. Efforts in our laboratory are directed at a more detailed understanding of factors that regulate EOC metastatic success. However, quantifying metastatic tumor burden represents a significant technical challenge due to the large number, small size and broad distribution of lesions throughout the peritoneum. Herein we describe a method for analysis of EOC metastasis using cells labeled with red fluorescent protein (RFP) coupled with in vivo multispectral imaging. Following intra-peritoneal injection of RFP-labelled tumor cells, mice are imaged weekly until time of sacrifice. At this time, the peritoneal cavity is surgically exposed and organs are imaged in situ. Dissected organs are then placed on a labeled transparent template and imaged ex vivo. Removal of tissue auto-fluorescence during image processing using multispectral unmixing enables accurate quantitation of relative tumor burden. This method has utility in a variety of applications including therapeutic studies to evaluate compounds that may inhibit metastasis and thereby improve overall survival.

Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.

腹腔内卵巣癌転移の定量

Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy in the United States.  Mortality is due to diagnosis of 75% of ...

Intraperitoneal Injection of Cells: A Method of Delivering Cancer Cells into the Peritoneal Adipose Tissue of Murine Model

Source: Krishnan, V. et al. <a href="https://jove.unicartagenaproxy.elogim.com/t/52721">Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose.</a> J. Vis. Exp. (2015)
In this video, we perform an intraperitoneal injection of ovarian cancer cell suspension in a mouse model to study the colonization of ovarian cancer cells in peritoneal adipose tissues and their subsequent metastasis.&#160;

細胞の腹腔内注入:マウスモデルの腹膜脂肪組織にがん細胞を送達する方法

Source: Krishnan, V. et al. Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose. J. Vis. Exp. 
...

オリジナル腫瘍の開発のマウス実験モデルと腹膜転移

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のための免疫担当マウスにおける漿液性卵巣癌の同所性モデル in vivoで腫瘍イメージングとモニタリング

An Orthotopic Mouse Model of Ovarian Cancer using Human Stroma to Promote Metastasis

同所性骨肉腫および肺転移マウスモデルを生成するための脛骨内骨肉腫細胞注射

卵巣がん再発を検出し、モニターするための非侵襲的イメージングのためのマウスモデル

ヒト大網を用いた卵巣癌腹膜転移の Ex vivo モデル

ヒト大網を用いた卵巣癌腹膜転移の Ex vivo モデル

ヒト大網を用いた卵巣癌腹膜転移の Ex vivo モデル

生体イメージングおよび卵巣癌の同所性マウスモデルにおける治療的処置の

腹腔内卵巣癌転移の定量

細胞の腹腔内注入:マウスモデルの腹膜脂肪組織にがん細胞を送達する方法