This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1.銀ナノ粒子の合成15 <ol><li> 5秒間100ミリリットルの超純水（18.2ΩU）とボルテックスで18 mgの硝酸銀を溶解させます。 </li><li> 5秒間1ミリリットルの水とボルテックスで27 mgのクエン酸ナトリウム二水和物を溶解させます。 </li><li>撹拌棒を含む三角フラスコに準備された硝酸銀溶液のすべてを転送し、磁気ホットプレート上にフラスコを置きます。 〜350℃で700rpmでの撹拌速度で激しく撹拌しながらフラスコを加熱（プレート上で温度を設定）。 </li><li>沸騰した場合、直ちに三角フラスコに準備されたクエン酸ナトリウム溶液のすべてを追加し、解決策は、Ag NPの形成を示す緑がかった茶色を、なるまでさらに25分間沸騰するソリューションを残します。 </li><li>ホットプレートからフラスコを外し、一定の色やトランで、別の磁性板の上に置いた（加熱しないでください）​​、攪拌O / NがRTで同じ撹拌速度で混合物が安定状態に達するまでsparency。必要に応じて用意したAg NPの吸光度を決定するために、紫外可視分光光度計を使用してください。 </li><li> 100mlに超純水を用いて最終混合物を希釈します。 </li><li>必要に応じて、製造業者のプロトコルに従ってのAg NPのサイズを測定するゼータサイザーを使用してください。 </li><li>密閉容器に銀コロイドを移し、アルミホイルで光から保護します。必要に応じてコロイドを2ヶ月間4-7℃の冷蔵庫に保存することができます。 </li></ol> SERSアクティブフィルタ膜の2製作<ol><li> 50mMのNaCl溶液を作るために水100mlに2.92はグラムの塩化ナトリウム（食塩）を溶解します。 </li><li>用意したAg NPの1ミリリットル中に5 mMのNaCl溶液の1ミリリットルを追加し、20 rpmで10分間章動ミキサーで混ぜます。このステップでは、Agナノクラスターの中に銀のNPを集約することです。 </li><li>注射器に取り付けることができるフィルタホルダにフィルター膜（PVDF、0.1μmの孔径）を置きます。より小さい孔サイズ膜FOました銀ナノクラスターを捕捉し、一貫性のある信号を生成する際に、より大きな細孔サイズの膜（ すなわち、0.22μmの）よりウントより効果的。 </li><li>濾過のための注射器にロード用意したAgナノクラスターの2ミリリットルを。シリンジにフィルターホルダーを取り付け、手動で1滴/秒の流量で膜を通してのAgナノクラスターのボリューム全体を渡します。膜トラップのAgナノクラスターは、SERS活性フィルター膜を形成します。 </li><li>フィルターホルダーからフィルター膜を外します。膜への損傷を受けないためにピンセットを使用して、外リムに膜を保持している場合には、特別な注意が必要とされています。スライドガラス上に約3分と場所膜用の空気乾燥。 </li><li> SERS基板のラマン検出<ol><li> 5mWと、10倍の顕微鏡対物レンズをセット2の1秒、露出数の露光時間のレーザパワーと波長780nmのレーザにラマン測定器を設定します。ソフトウェア上の目的は、あまりにもそれに応じて設定されていることを確認します。&lt;/李&gt; <li>ラマン計測器のプラットフォーム上に上に膜を有するガラススライドを配置し、膜の表面に集中する顕微鏡を使用しています。 </li><li>ランダム膜表面8から10のスポットを選択し、機器が順番に自動的にそれらを収集します。分析のための製造元のソフトウェアでのスペクトルデータを開きます。 </li></ol></li></ol>化学汚染物質を検出するために、SERSアクティブフィルタシステムの3.アプリケーション<ol><li> 10 ppbでのフェルバム溶液を調製します。 注意：フェルバムは非常に揮発性です。固体を計量する際の注意事項（人工呼吸器やゴーグル）を使用します。 <ol><li> 2ミリグラムフェルバム粉末を秤量し、ストック溶液（100 ppm）を行う20 50mlの％アセトニトリル（10mLのアセトニトリルおよび10mlの水）の中に溶解します。 30秒間フラスコを渦。 </li><li>試験管に100ppmのフェルバム溶液1mlを取り、10ppmの溶液を作る9 50mlの％アセトニトリルを加えます。 5秒間チューブをボルテックス。 </li><li>の1ミリリットルを取り10 ppmの試験管内の溶液と9ミリリットルの50％アセトニトリルを追加するには、1 ppmの溶液を作製します。 5秒間チューブをボルテックス。 </li><li>試験管内では1ppm溶液1mlを取り、100ppbの溶液を作製するために9ミリリットルの50％アセトニトリルを追加します。 5秒間チューブをボルテックス。 </li><li>試験管内で100ppbの溶液1mlを取り、10 ppbの溶液を作製するために9ミリリットルの50％アセトニトリルを追加します。 5秒間チューブをボルテックス。 </li></ol></li><li> 1ppmのアンピシリン溶液を調製します。 <ol><li> 10 mgのアンピシリン粉末を秤量し、100ppmのアンピシリン溶液を作るために100 mLの水に溶解します。 30秒間フラスコを渦。 </li><li>試験管内で100ppmの溶液1mlを取り、10 ppmのアンピシリン溶液を作製するために9ミリリットルの水を追加します。 5秒間チューブをボルテックス。 </li><li>試験管に10ppmの溶液1mlを取り、1 ppmのアンピシリン溶液を作製するために9ミリリットルの水を追加します。 5秒間チューブをボルテックス。 </li></ol></li><li> NPコーティングされた側を上に向けて、バックフィルターホルダーにフィルター膜を置きます。&lt;/李&gt; <li>その後、新しいシリンジにロード一つのサンプルの5ミリリットルを、そして内側の銀コーティングされた膜でフィルターホルダーに取り付けます。 </li><li>手動で1滴/秒の流量で膜を通してサンプルのボリューム全体を渡します。標的分子は、フィルター膜上に塗布されたNPを吸着させ、濃縮することができます。 </li><li> 、フィルターホルダーからフィルタ膜を取り外し、約3分間空気乾燥し、ステップ2.6に記載したのと同じ方法を用いて、ラマン装置を使用して信号を測定します。 </li><li>別の銀コーティングされた膜を作成し、他のサンプルの検出のためのステップ3.3から追従する2.6を繰り返しステップ2.2。 </li></ol>

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The authors have nothing to disclose.

このプロトコルにおいて重要なステップの一つは、均一のAg NPは一貫性のある結果を得るための鍵である銀のNP合成、です。加熱時間及び前駆体の濃度を正確に制御しなければなりません。このAgNPs製剤の平均サイズはゼータサイザーで測定した80ナノメートルである（データは示さず）。別の重要なステップは、塩濃度及び凝集時間を正確に制御しなければならない塩の集合体です。小さい?...

表面増強ラマン分光法（SERS）は、ナノテクノロジーを用いて、ラマン分光法を組み合わせた手法です。貴金属ナノ表面における検体のラマン散乱光の強度が大幅に局在表面プラズモン共鳴により増強される。1銀ナノ粒子（銀NPS）その高い拡張能力をはるかに最も広く使用されているSERS用基板である。今まで2 Ag NPの様々な合成方法が開発されている。3-6のAg NPは、その感度および/ ​​または機能を増強するのに有効なSERS基板として単独で使用、または他の材料及び構造と組み合わせることができる。7-11 SERS技術は、食品や環境試料中の様々な微量汚染物質の検出のための偉大な能力を実証している12は伝統的に、SERSのサンプルを調製するための2つの一般的な方法があります：。。溶液系と基板ベースの方法は、13 ソリューションベースのメ​​トdは、サンプルと混合するNPコロイドを使用しています。次にNP - 分析物複合体は、遠心分離を用いて回収し、乾燥後のラマン測定のための固体支持体上に堆積されます。基板ベースの方法は、通常、事前に作製した固体基板上に液体試料のいくつかのマイクロリットルを堆積させることによって適用される。14が、これら2つの方法のいずれもが、試料容量の大量のために有効かつ適用可能です。 SERSアッセイのいくつかの変更は、フィルターシステム15-21またはマイクロ流体デバイスの組み込みの統合などの音量制限を、克服した。21-24修正されたSERSアッセイは、化学汚染物質を監視するための感度と実現可能性に大きな向上を示しています大規模な水サンプルインチここでは、農薬フェルバムおよび抗生物質アンピシリンの微量を検出するために製造およびシリンジフィルターベースのSERS法の適用の詳細なプロトコルを示します。

<table><tbody><tr><td>Ampicillin</td><td>Fisher Scientific</td><td>BP1760-5</td><td>N/A</td></tr><tr><td>Ferbam</td><td>Chem Service</td><td>N-11970-250MG</td><td>98+%</td></tr><tr><td>Silver nitrate</td><td>Sigma Aldrich</td><td>209139</td><td>99.0+%</td></tr><tr><td>Sodium citrate dehydrate</td><td>Sigma Aldrich</td><td>W302600</td><td>99+%</td></tr><tr><td>Sodium chloride</td><td>Sigma Aldrich</td><td>S7653</td><td>99.5+%</td></tr><tr><td>EMD Millipore Durapore PVDF Membrane Filters</td><td>Fisher Scientific</td><td>VVLP01300</td><td>0.10 &amp;micro;m Pore Size, hydrophilic</td></tr><tr><td>Polycarbonate Filter Holders</td><td>Cole-Parmer</td><td>EW-29550-40</td><td>13 mm diameter</td></tr><tr><td>Analog Vortex Mixer</td><td>Fisher Scientific</td><td>02-215-365</td><td>N/A</td></tr><tr><td>Nutating Mixers</td><td>Fisher Scientific</td><td>05-450-213</td><td>N/A</td></tr><tr><td>DXR Raman spectroscope</td><td>Thermo Scientific</td><td>IQLAADGABFFAHCMAPB</td><td>Laser power: 1 mW&lt;br /&gt; Exposure time: 5 sec</td></tr></tbody></table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

この実験の主要な工程を示す模式図（ 図1）に示した。2が最大感度に達するために、膜コーティングでAgNPsの最適化されたボリュームを使用する重要性を示した図 。フェルバムを使用している場合0.5ミリリットル（不十分なコーティング）または2ミリリットル（あまりにも多くのコーティング）と比較したAg NPの1ミリリットルは...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

製造や化学汚染物質（ すなわち、農薬フェルバムと抗生物質アンピシリン）の検出のためのラマン分光（SERS）アッセイ強化フィルタベースの表面を実行するための手順が提示されています。

化学汚染物質の迅速検出のためのラマン分光アッセイ強化フィルターベースの表面

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

a-filter-based-surface-enhanced-raman-spectroscopic-assay-for-rapid

Universidad de Cartagena UDEC

Research

JoVE Journal

Chemistry

9.3K ビュー.  University of Massachusetts Amherst. 製造や化学汚染物質（ すなわち、農薬フェルバムと抗生物質アンピシリン）の検出のためのラマン分光（SERS）アッセイ強化フィルタベースの表面を実行するための手順が提示されています。 

ビデオ: A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, a large infrastructure of immunoassay instruments and materials can be found. For example, 96- and 384-well polystyrene plates are available commercially and have a standard design to accommodate ultraviolet-visible (UV-Vis) spectroscopy machines from various manufacturers. In addition, a wide variety of immunoglobulins, detection tags, and blocking agents for customized immunoassay designs such as enzyme-linked immunosorbent assays (ELISA) are available.
Despite the existing infrastructure, standard ELISA kits do not meet all research needs, requiring individualized immunoassay development, which can be expensive and time-consuming. For example, ELISA kits have low multiplexing (detection of more than one analyte at a time) capabilities as they usually depend on fluorescence or colorimetric methods for detection. Colorimetric and fluorescent-based analyses have limited multiplexing capabilities due to broad spectral peaks. In contrast, Raman spectroscopy-based methods have a much greater capability for multiplexing due to narrow emission peaks. Another advantage of Raman spectroscopy is that Raman reporters experience significantly less photobleaching than fluorescent tags1. Despite the advantages that Raman reporters have over fluorescent and colorimetric tags, protocols to fabricate Raman-based immunoassays are limited. The purpose of this paper is to provide a protocol to prepare functionalized probes to use in conjunction with polystyrene plates for direct detection of analytes by UV-Vis analysis and Raman spectroscopy. This protocol will allow researchers to take a do-it-yourself approach for future multi-analyte detection while capitalizing on pre-established infrastructure.

Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.

紫外可視およびラマン分光法イムノアッセイプラットフォームを製作

Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, ...

生化学

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, and the results of SERS imaging are reported. The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated. The results also illustrate the influence of the surface nanostructure geometry, biomolecules immobilization strategy, Raman activity of the molecules and presence or absence of the ligand binding on the SERS spectra acquired.

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

表面EBL作製したナノ構造基板を用いた生体分子のラマン分光検出の強化

Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules ...

工学

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, and that these chemical fingerprints can be used for plant species identification. The mass spectral data can be acquired rapidly and in a high throughput manner without the need for sample extraction, derivatization or pH adjustment steps. The use of this technique bypasses challenges presented by more conventional techniques including lengthy chromatography analysis times and resource intensive methods. The high throughput capabilities of the direct analysis in real time-high resolution mass spectrometry protocol, coupled with multivariate statistical analysis processing of the data, provide not only class characterization of plants, but also yield species and varietal information. Here, the technique is demonstrated with two psychoactive plant products, Mitragyna speciosa (Kratom) and Datura (Jimsonweed), which were subjected to direct analysis in real time-high resolution mass spectrometry followed by statistical analysis processing of the mass spectral data. The application of these tools in tandem enabled the plant materials to be rapidly identified at the level of variety and species.

A method for species identification of botanical material by direct analysis in real time-high resolution mass spectrometry and multivariate statistical analysis is presented.

リアルタイム高分解能質量分析法で直接分析を用いた植物材料の急速なハイスループット種の同定

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, ...

化学

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, when used together, can indicate the presence of gunshot residue (GSR) by producing a fluorescence signal in the presence of three key analytes (antimony, lead, and organic components of GSR). The protocol describes the synthesis of the biosensors using Escherichia coli (E. coli), and the analytical chemistry methods used to evaluate the selectivity and sensitivity of the sensors. The functioning of the system is demonstrated by using GSR collected from the inside of a spent cartridge casing. Once prepared, the biosensors can be stored until needed and can be used as a test for these key analytes. A positive response from all three analytes provides a presumptive positive test for GSR, while each individual device has applications for detecting the analytes in other samples (e.g., a detector for lead contamination in drinking water). The main limitation of the system is the time required for a positive signal; future work may involve studying different organisms to optimize the response time.

A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

発砲残渣の推定検出のための新しい細菌性バイオセンサの調製と適用

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, ...

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical technique for label-free chemical imaging. This analytical tool delivers chemical maps at high speed, and high spatial resolution of thin samples by directly interrogating their molecular vibrations. In its standard implementation, SRS microscopy is narrowband and forms images with only a single vibrational frequency at a time. However, this approach not only hinders the chemical specificity of SRS but also neglects the wealth of information encoded within vibrational spectra. 
These limitations can be overcome by broadband SRS, an implementation capable of extracting a vibrational spectrum per pixel of the image in parallel. This delivers hyperspectral data that, when coupled with chemometric analysis, maximizes the amount of information retrieved from the specimen. Thus, broadband SRS improves the chemical specificity of the system, allowing the quantitative determination of the concentration of the different constituents of a sample. Here, we report a protocol for chemical imaging with broadband SRS microscopy, based on a home-built SRS microscope operating with a custom differential multichannel-lock-in amplifier detection. It discusses the sample preparation, alignment of the SRS apparatus, and chemometric analysis. By acquiring vibrational Raman spectra, the protocol illustrates how to identify different chemical species within a mixture, determining their relative concentrations.

We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

広帯域刺激ラマン散乱顕微鏡に基づく多重化学イメージング

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical technique for label-free chemical imaging. This analytical tool delivers chemical maps ...

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging technologies. Hyperspectral SRS and CARS imaging offer Raman spectral information at every pixel, which enables better separation of different chemical compositions. Although both techniques require two excitation lasers, their signal detection schemes and spectral properties are quite different. The goal of this protocol is to perform both hyperspectral SRS and CARS imaging on a single platform and compare the two microscopy techniques for imaging different biological samples. The spectral focusing method is employed to acquire spectral information using femtosecond lasers. By using standard chemical samples, the sensitivity, spatial resolution, and spectral resolution of SRS and CARS in the same excitation conditions (i.e., power at the sample, pixel dwell time, objective lens, pulse energy) are compared. The imaging contrasts of CARS and SRS for biological samples are juxtaposed and compared. The direct comparison of CARS and SRS performances would allow for optimal selection of the modality for chemical imaging.

This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity.

化学イメージングのためのハイパースペクトル刺激ラマン散乱とコヒーレントアンチストークスラマン散乱顕微鏡の直接比較

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging ...

レーザーアブレーションによるナノ粒子とナノ構造の合成

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

The technique of ultrafast laser ablation in liquids has evolved and matured over the past decade, with several impending applications in various fields such as sensing, catalysis, and medicine. The exceptional feature of this technique is the formation of nanoparticles (colloids) and nanostructures (solids) in a single experiment with ultrashort laser pulses. We have been working on this technique for the past few years, investigating its potential using the surface-enhanced Raman scattering (SERS) technique in hazardous materials sensing applications. Ultrafast laser-ablated substrates (solids and colloids) could detect several analyte molecules at the trace levels/mixture form, including dyes, explosives, pesticides, and biomolecules. Here, we present some of the results achieved using the targets of Ag, Au, Ag-Au, and Si. We have optimized the nanostructures (NSs) and nanoparticles (NPs) obtained (in liquids and air) using different pulse durations, wavelengths, energies, pulse shapes, and writing geometries. Thus, various NSs and NPs were tested for their efficiency in sensing numerous analyte molecules using a simple, portable Raman spectrometer. This methodology, once optimized, paves the way for on-field sensing applications. We discuss the protocols in (a) synthesizing the NPs/NSs via laser ablation, (b) characterization of NPs/NSs, and (c) their utilization in the SERS-based sensing studies.

著者スポットライト:液体中のレーザーアブレーションによるナノ粒子合成の進歩と応用 

Ultrafast laser ablation in liquid is a precise and versatile technique for synthesizing nanomaterials (nanoparticles [NPs] and nanostructures [NSs]) in liquid/air environments. The laser-ablated nanomaterials can be functionalized with Raman-active molecules to enhance the Raman signal of analytes placed on or near the NSs/NPs.

表面増強ラマン散乱ベースのセンシングアプリケーションのための超高速レーザーアブレーションナノ粒子およびナノ構造

The technique of ultrafast laser ablation in liquids has evolved and matured over the past decade, with several impending applications in various fields ...

R6GおよびThiramのSERS検出用フレキシブル基板の作製

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

This article presents a fabrication method for a flexible substrate designed for Surface-Enhanced Raman Scattering (SERS). Silver nanoparticles (AgNPs) were synthesized through a complexation reaction involving silver nitrate (AgNO3) and ammonia, followed by reduction using glucose. The resulting AgNPs exhibited a uniform size distribution ranging from 20 nm to 50 nm. Subsequently, 3-aminopropyl triethoxysilane (APTES) was employed to modify a PDMS substrate that had been surface-treated with oxygen plasma. This process facilitated the self-assembly of AgNPs onto the substrate. A systematic evaluation of the impact of various experimental conditions on substrate performance led to the development of a SERS substrate with excellent performance and an Enhanced Factor (EF). Utilizing this substrate, impressive detection limits of 10-10 M for R6G (Rhodamine 6G) and 10-8 M for Thiram were achieved. The substrate was successfully employed for detecting pesticide residues on apples, yielding highly satisfactory results. The flexible SERS substrate demonstrates great potential for real-world applications, including detection in complex scenarios.

著者スポットライト:合成AgNPを用いたSERSフレキシブル基板の開発と応用 

This protocol describes a fabrication method for a flexible substrate for surface-enhanced Raman scattering. This method has been used in the successful detection of low concentrations of R6G and Thiram.

超高感度検出のためのポリジメチルシロキサン(PDMS)ベースのフレキシブル表面増強ラマン散乱(SERS)基板の作製

This article presents a fabrication method for a flexible substrate designed for Surface-Enhanced Raman Scattering (SERS). Silver nanoparticles (AgNPs) ...

化学汚染物質の迅速検出のためのラマン分光アッセイ強化フィルターベースの表面

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紫外可視およびラマン分光法イムノアッセイプラットフォームを製作

表面EBL作製したナノ構造基板を用いた生体分子のラマン分光検出の強化

リアルタイム高分解能質量分析法で直接分析を用いた植物材料の急速なハイスループット種の同定

発砲残渣の推定検出のための新しい細菌性バイオセンサの調製と適用

広帯域刺激ラマン散乱顕微鏡に基づく多重化学イメージング

化学イメージングのためのハイパースペクトル刺激ラマン散乱とコヒーレントアンチストークスラマン散乱顕微鏡の直接比較

表面増強ラマン散乱ベースのセンシングアプリケーションのための超高速レーザーアブレーションナノ粒子およびナノ構造

表面増強ラマン散乱ベースのセンシングアプリケーションのための超高速レーザーアブレーションナノ粒子およびナノ構造

表面増強ラマン散乱ベースのセンシングアプリケーションのための超高速レーザーアブレーションナノ粒子およびナノ構造

超高感度検出のためのポリジメチルシロキサン(PDMS)ベースのフレキシブル表面増強ラマン散乱(SERS)基板の作製