定量的グライコミクスおよびプロテオミクス複合精製戦略

1:10

Protein and Glycoprotein Denaturation and Alkylation

2:15

N-linked Glycan Enzymatic Digestion

3:29

Elution of N-glycans and Protein Filter Aided Sample Preparation (FASP) Digestion

6:37

Derivatization of N-linked Glycans with Hydrophobic Hydrazide Tags

8:19

Ultra-high Pressure Liquid Chromatography and Mass Spectrometry Analysis

9:27

Results: Analysis of the Glycomics and Proteomics Combined Purification Strategy

10:44

Conclusion