Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived XenotransplantsVictor Gife 1,2, Bahram Sharif-Askari 3, Anavasadat Sadr Hashemi Nejad 1,2, Raquel Aloyz 3,4,5, Laura Hulea 1,2,6, François E. Mercier 3,4
1Maisonneuve-Rosemont Hospital Research Centre, 2Department of Biochemistry and Molecular Medicine, University of Montreal, 3Lady Davis Institute for Medical Research, 4Department of Medicine, McGill University, 5Gerald Bronfman Department of Oncology, McGill University, 6Department of Medicine, University of Montreal
Here, a phosphoflow cytometry-based method is described to analyze signaling downstream of the mTORC1, JAK/STAT5, and MAPK pathways in acute human myeloid leukemia cells xenografted into mice and obtained from bone marrow aspirates. p-STAT5, p-4EBP1, p-RPS6, and p-ERK1/2 levels are simultaneously measured using a next-generation spectral flow cytometer with high sensitivity.
