Suining Central Hospital

3 ARTICLES PUBLISHED IN JoVE

Medicine

Exploring the Pharmacological Action and Molecular Mechanism of Salidroside in Inhibiting MCF-7 Cell Proliferation and Migration

Lijuan Cui¹, Chuan Ye¹, Ting Luo¹, Hongyan Jiang¹, Boan Lai¹, Haojun Wang¹, Zhongyi Chen¹, Yi Li²

¹Pathology Department, Suining Central Hospital, ²General Surgery Day Ward, Department of General Surgery, the Third People's Hospital of Chengdu & the Affiliated Hospital of Southwest Jiaotong University

The present protocol describesa comprehensive strategy for evaluating the pharmacological action and mechanism of salidroside in inhibiting MCF-7 cell proliferation and migration.

Biology

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

Ming Yang^*1,2, Lijuan Cui^*3, Xuesha Liu¹

¹College of Life Sciences, China West Normal University, ²Sichuan Sports College, ³Pathology Department, Suining Central Hospital

Here, we present a protocol to obtain the pVAX1-PRRSV expression vector by introducing suitable restriction sites at the 3' end of the inserts. We can linearize the vector and join DNA fragments to the vector one by one through homologous recombination technology.

Biochemistry

Separation and Differential Characterization of Gut Microbial Extracellular Vesicles in Salt-Sensitive Rats under High-Salt Diet Conditions

Lisha Wang^*1, Lijuan Cui^*2, Luming Qi³, Youli Tan⁴, Shuyu Wang³, Hongling Liu¹, Changhong Wei³, Zhangmeng Xu⁵, Jie Wang⁶

¹School of Medical Technology, Chengdu University of Traditional Chinese Medicine, ²Pathology Department, Suining Central Hospital, ³School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, ⁴Department of Pharmacy, Affiliated Sport Hospital of CDSU, ⁵Department of Neck, Shoulder, Waist, and Leg Pain, Sichuan Province Orthopedic Hospital, ⁶Neijiang Hospital of Traditional Chinese Medicine Affiliated to Chengdu University of Traditional Chinese Medicine

The protocol describes the isolation of gut microbial EVs from salt-sensitive rats fed HSD using density gradient centrifugation. EVs were characterized by nanoparticle tracking, TEM, LPS/BCA assays, and 16S rRNA sequencing to analyze the size, morphology, composition, and microbiota origin.