Utilizzando CRISPR/Cas9 Gene Editing per studiare l'attività oncogena di calreticulina mutante in cellule ematopoietiche Cytokine dipendente

0:44

Cloning sgRNA Oligos

1:08

Digestion of LentiGuide-puroVector

2:04

Ligation of Annealed Oligos into Digested Backbone

3:02

Generation of Cell Lines Stably Expressing spCas9

5:40

Reporter Assay for Cas9 Activity

6:10

Spinfection of sgRNAs into Cas9 Expressing Cells

6:31

Ba/F3 Cellular Transformation and Positive Selection using m-IL3 Withdrawal

7:23

Results: Use of CRISPR/Cas9 Tool and Ba/F3 Cellular Transformation to Study Oncogenic Potential of Mutated Calreticulin

9:02

Conclusion