JoVE Logo

Accedi

Chapter 32

Analyzing Cells and Proteins

Panoramica sulla separazione e l'isolamento cellulare
Panoramica sulla separazione e l'isolamento cellulare
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, ...
Coltura cellulare
Coltura cellulare
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are ...
Linee cellulari
Linee cellulari
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number ...
Tecnologia degli ibridomi
Tecnologia degli ibridomi
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or ...
Omogeneizzazione tissutale e lisi cellulare
Omogeneizzazione tissutale e lisi cellulare
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. ...
Frazionamento subcellulare
Frazionamento subcellulare
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular ...
Citometria a flusso
Citometria a flusso
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing ...
Principi della cromatografia su colonna
Principi della cromatografia su colonna
The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. ...
Tipi di cromatografia su colonna
Tipi di cromatografia su colonna
The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating ...
Immunoprecipitazione
Immunoprecipitazione
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native ...
Marcatura e fusione delle proteine
Marcatura e fusione delle proteine
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from ...
PAGINA SDS
PAGINA SDS
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under ...
Western Blotting
Western Blotting
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting ...
Elettroforesi su gel bidimensionale
Elettroforesi su gel bidimensionale
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method ...
Saggio di immunoassorbimento enzimatico
Saggio di immunoassorbimento enzimatico
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays ...
Spettrometria di massa MALDI-TOF
Spettrometria di massa MALDI-TOF
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological ...
Identificazione dei peptidi mediante spettrometria di massa tandem
Identificazione dei peptidi mediante spettrometria di massa tandem
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass ...
Diffrazione a raggi X di campioni biologici
Diffrazione a raggi X di campioni biologici
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, ...
Applicazioni della risonanza magnetica nucleare in biologia
Applicazioni della risonanza magnetica nucleare in biologia
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an ...
Proteomica
Proteomica
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, ...
JoVE Logo

Riservatezza

Condizioni di utilizzo

Politiche

Ricerca

Didattica

CHI SIAMO

Copyright © 2025 MyJoVE Corporation. Tutti i diritti riservati