Separation and Differential Characterization of Gut Microbial Extracellular Vesicles in Salt-Sensitive Rats under High-Salt Diet ConditionsLisha Wang *1, Lijuan Cui *2, Luming Qi 3, Youli Tan 4, Shuyu Wang 3, Hongling Liu 1, Changhong Wei 3, Zhangmeng Xu 5, Jie Wang 6
1School of Medical Technology, Chengdu University of Traditional Chinese Medicine, 2Pathology Department, Suining Central Hospital, 3School of Health Preservation and Rehabilitation, Chengdu University of Traditional Chinese Medicine, 4Department of Pharmacy, Affiliated Sport Hospital of CDSU, 5Department of Neck, Shoulder, Waist, and Leg Pain, Sichuan Province Orthopedic Hospital, 6Neijiang Hospital of Traditional Chinese Medicine Affiliated to Chengdu University of Traditional Chinese Medicine
The protocol describes the isolation of gut microbial EVs from salt-sensitive rats fed HSD using density gradient centrifugation. EVs were characterized by nanoparticle tracking, TEM, LPS/BCA assays, and 16S rRNA sequencing to analyze the size, morphology, composition, and microbiota origin.
