Isolation, Fixation and Characterization of Juvenile Gilthead Seabream Head Kidney Leukocytes by Flow CytometryIsa Marmelo 1,2,3, Zélia Silva 4,5, Daniel Bolotas 2, Ricardo N. Alves 6, Paula A. Videira 4,5,7, António Marques 2,3, Mário Sousa Diniz 1,5, Ana Luísa Maulvault 1,2,5
1UCIBIO - Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University of Lisbon, 2IPMA DivAV - Division of Aquaculture, Upgrading and Bioprospection, Portuguese Institute for the Sea and Atmosphere, 3CIIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, 4UCIBIO - Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, NOVA University of Lisbon, 5Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University of Lisbon, 6Bioscience Core Lab, King Abdullah University of Science and Technology, 7CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Department of Life Sciences, NOVA School of Science and Technology, NOVA University of Lisbon
This manuscript describes the isolation and fixation of leukocytes extracted from gilthead seabream's head-kidney and the assessment of their viability by flow cytometry. This work contributes to the standardization of protocols and leverages the processing of a higher number of samples without compromising sample quality, promoting advancements in fish immunology knowledge.
