Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

1:28

Isolation of Newborn Inguinal Lymph Nodes (LN)

2:38

Isolation of E18.5 Fat Pads

3:28

Generation of the LN-fat Pad Chimera

5:14

Isolation and Enzymatic Digestion of the Transferred LN for Flow Cytometric Analysis

6:31

Analysis of the Transferred LNs by Immunofluorescence

7:16

Results: Typical Experimental Results Obtained Using the LN/fat Pad Chimera Method

8:44

Conclusion