University of California, San Francisco (UCSF)

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Unique Mouse Model for Quantitative Assessment of Biofilm Formation on Surgical Implants in Subcutaneous Abscess

Mitsuhiro Nishizawa¹, Bahram Saleh², Ralph Marcucio^*1, Kazuhito Morioka^*1,3

¹Department of Orthopaedic Surgery, Orthopaedic Trauma Institute (OTI), University of California, San Francisco (UCSF), ²Rosies Base, LLC, ³Department of Neurological Surgery, Weill Institute for Neurosciences, Brain and Spinal Injury Center (BASIC), University of California, San Francisco (UCSF)

This protocol describes a unique experimental model of implant-related infections that enables the simultaneous incubation of two implants with bacteria under identical conditions within a single mouse. It also allows precise assessment of biofilm formation on implant surfaces using optimized comparative analytical methods, demonstrating advanced techniques for evaluating biomaterials' antimicrobial properties.