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12:25 min
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August 31st, 2012
DOI :
August 31st, 2012
•0:05
Title
2:08
Preparation of the Membrane Scaffold Protein, MSP
3:53
Preparation of the MalFGK2 Complex
5:09
Preparation of Phospholipids
6:07
Preparation of Bio-Beads & Nanodisc Reconstitution
7:59
Nanodisc Analysis: Native Gel Electrophoresis
8:26
Nanodisc Analysis: Dynamic Light Scattering (DLS)
9:11
Nanodisc Analysis: ATPase Measurements
9:47
Results: Activity of the maltose Transporter Reconstituted into Nanodiscs
11:15
Conclusion
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