In Vitro Culture for H5N1-Specific Duck T Cells and Detection of Immune Responses Using Intracellular Cytokine Staining MethodXueqing Li *1,2, Zimin Xie *1,2, Wanlin Jiao *1,2, Ming Liao 1,2,3, Manman Dai 1,2,3
1National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, 2National Avian Influenza Para-Reference Laboratory, 3UK-China Centre of Excellence for Research on Avian Diseases
The protocol describes a method for culturing avian T cells in vitro by isolating memory T cells from infected ducks, allowing for the generation of highly purified specific duck T cells. Additionally, an intracellular cytokine staining (ICS) method was established to accurately measure IFN-γ secretion in duck T cells.
