Iniciar sesión

Lady Davis Institute for Medical Research

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy

Robert J. Scarborough^1,2, Kelsey L. Adams^1,2, Olivier Del Corpo^1,2, Aïcha Daher¹, Anne Gatignol^1,2,3

¹Virus-Cell Interactions Laboratory, Lady Davis Institute for Medical Research, ²Department of Microbiology & Immunology, McGill University, ³Department of Medicine, McGill University

Methods to evaluate the efficacy and toxicity of RNA molecules targeting post-integration steps of the HIV-1 replication cycle are described. These methods are useful for screening new molecules and optimizing the format of existing ones.

Biology

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Victor Gife^1,2, Bahram Sharif-Askari³, Anavasadat Sadr Hashemi Nejad^1,2, Raquel Aloyz^3,4,5, Laura Hulea^1,2,6, François E. Mercier^3,4

¹Maisonneuve-Rosemont Hospital Research Centre, ²Department of Biochemistry and Molecular Medicine, University of Montreal, ³Lady Davis Institute for Medical Research, ⁴Department of Medicine, McGill University, ⁵Gerald Bronfman Department of Oncology, McGill University, ⁶Department of Medicine, University of Montreal

Here, a phosphoflow cytometry-based method is described to analyze signaling downstream of the mTORC1, JAK/STAT5, and MAPK pathways in acute human myeloid leukemia cells xenografted into mice and obtained from bone marrow aspirates. p-STAT5, p-4EBP1, p-RPS6, and p-ERK1/2 levels are simultaneously measured using a next-generation spectral flow cytometer with high sensitivity.