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Chemical Genomics Centre of the Max Planck Society

1 ARTICLES PUBLISHED IN JoVE

Genetics

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Jeffrey L. Schloßhauer^*1,2, Sama Shamloo^*1,2, Katja Schamrin^1,2, Jochen Imig^1,2

¹Chemical Genomics Centre of the Max Planck Society, ²Max Planck Institute of Molecular Physiology

This study presents a dual CRISPRi system targeting long non-coding RNAs in melanoma cells. It enables combinatorial gene knockdown and synthetic lethal screening, identifying cancer-specific lncRNA interactions for potential therapeutic strategies.