Science Education - Advanced Biology
Genetics
10:04An Overview of Genetic Analysis
An organism’s physical traits, or phenotype, are a product of its genotype, which is the combination of alleles (gene variants) inherited from its ...
08:45Genetic Crosses
To dissect genetic processes or create organisms with novel suites of traits, scientists can perform genetic crosses, or the purposeful mating of two ...
08:50Genetic Screens
Genetic screens are critical tools for defining gene function and understanding gene interactions. Screens typically involve mutating genes and then ...
10:47An Overview of Genetics and Disease
Many human diseases are associated with mutations or variations in genetic sequences. Some of these genetic variants are heritable, passed down from ...
08:23SNP Genotyping
Single nucleotide polymorphisms, or SNPs, are the most common form of genetic variation in humans. These differences at individual bases in the DNA often ...
08:31Cytogenetics
Cytogenetics is the field of study devoted to chromosomes, and involves the direct observation of a cell’s chromosomal number and structure, ...
11:03An Overview of Gene Expression
Gene expression is the complex process where a cell uses its genetic information to make functional products. This process is regulated at multiple ...
08:29Expression Profiling with Microarrays
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and ...
09:44Rapid Amplification of cDNA Ends
Source: Pablo Sanchez Bosch2, Sean Corcoran2 and Katja Brückner1,2,3 1Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research ...
09:54RNA-Seq
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed ...
10:21An Overview of Epigenetics
Since the early days of genetics research, scientists have noted certain heritable phenotypic differences that are not due to differences in the ...
08:47DNA Methylation Analysis
Methylation at CpG dinucleotides is a chemical modification of DNA hypothesized to play important roles in regulating gene expression. In particular, the ...
08:39Chromatin Immunoprecipitation
Histones are proteins that help organize DNA in eukaryotic nuclei by serving as “scaffolds” around which DNA can be wrapped, forming a complex ...
10:38An Overview of Genetic Engineering
Genetic engineering – the process of purposefully altering an organism’s DNA – has been used to create powerful research tools and model ...
08:56Recombineering and Gene Targeting
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that ...
08:31Genome Editing
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be ...
