Spatula Montevideo Device for the Vitrification of Mammalian EmbryosGeraldine Schlapp *1, María Noel Meikle *1, Lucía Goyeneche 2, Pedro Dos Santos Neto 3, Martina Crispo 1
1Laboratory Animal Biotechnology Unit, Institut Pasteur de Montevideo, 2Centro de Esterilidad Montevideo, 3Fundación IRAUy, Instituto de Reproducción Animal de Uruguay
Here, we present the Spatula Montevideo protocol for vitrifying mouse and sheep pre-implantation embryos, consisting of a homemade spatula and utilizing homemade solutions. Embryos are rapidly equilibrated in two cryoprotectant solutions of increasing concentration, loaded onto the spatula, immersed in liquid nitrogen, and finally sealed with a 0.5 mL straw.
