Name: 视频: 兔模型气管插管:将气管插管插入兔气管的程序 - 概念
Uploaded: 2023-04-30T14:51:59.000+00:00
Duration: 1 min 35 s
Description: 1.7K 次观看. 来源:Pacheco-Baltazar A 等人 兔模型中的离体肺灌注系统。J. Vis. Exp. （2021）。 该视频演示了在兔气管中手术创建一个开口，以插入气管插管，用于人工通气肺部。

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All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Surgical extraction of the cardiopulmonary block.
<ol>
	<li>Anesthesia:
	<ol>
		<li>Use a combination of a sedative (xylazine) and a barbiturate (pentobarbital). 
		NOTE: Different anesthetic cocktails can be used with no effect on experimental outcomes.</li>
		<li>First, sedate the healthy New Zealand rabbits with a single intramuscular injection of xylazine hydrochloride (3-5 mg/kg). Ensure that the rabbits remain calm and relaxed to allow further manipulation after a few minutes of the injection.</li>
		<li>Following sedation, use the marginal (lateral) ear veins as access to anesthetize the rabbits with an intravenous injection of pentobarbital sodium (28 mg/kg).</li>
	</ol>
	</li>
	<li>Monitoring:
	<ol>
		<li>To avoid insufficient anesthesia or excessive depression of cardiac and respiratory functions, monitor the following parameters. To assess the depth of anesthesia, perform a toe pinch test.</li>
		<li>Ensure that the mucous membrane is pink. Blue or grey shades indicate hypoxia.</li>
		<li>Ensure that the heart rate is between 120-135 beats/min and that the body temperature does not drop below 36.5 &#176;C.</li>
	</ol>
	</li>
	<li>Animal placement:
	<ol>
		<li>Shave the rabbit&#39;s torso and place the animal on the operating table in the supine position. Place the ventilation system near the table, behind the rabbit&#39;s head, to permit connecting the cannula quickly after tracheotomy to avoid tissular damage.</li>
	</ol>
	</li>
	<li>Incision and tracheotomy:
	<ol>
		<li>Dissect the skin with a ventral median line incision of 3-5 cm from the diaphragm up to the neck.</li>
		<li>With the operating scissors, cut the anterior 2/3 of the trachea between two cartilage rings to insert the tracheal cannula through the tracheal fibrous membrane.</li>
		<li>Insert a 5 mm (outer diameter; OD) tracheal cannula through the tracheal fibrous membrane and use a 4-0 silk suture to fix it carefully.</li>
		<li>Place either forceps or tweezers underneath the trachea to ensure the cannula did not bend against the trachea.</li>
	</ol>
	</li>
</ol>

<table><tbody><tr><td>New Zeland rabbits</td><td>NA</td><td>NA</td><td></td></tr><tr><td>Tracheal Cannula for Rabbit, OD 5.0 mm&amp;nbsp;</td><td>Hugo Sachs Elektronik (HSE)&amp;nbsp;</td><td>73-4163</td><td></td></tr><tr><td>PROCIN (Xylacine clorhydrate)&amp;nbsp;</td><td>PISA&amp;nbsp;</td><td>Q-7833-099</td><td></td></tr><tr><td>PISABENTAL (Pentobarbital sodium)</td><td>&amp;nbsp;PISA&amp;nbsp;</td><td>Q-7833-215</td><td></td></tr><tr><td>Scalpel knife</td><td>NA</td><td>NA</td><td></td></tr><tr><td>Surgical gloves No. 8</td><td>NA</td><td>NA</td><td></td></tr></tbody></table>

tracheal cannulation in rabbit model: a procedure to insert tracheal cannula into the rabbit trachea

Source: Pacheco-Baltazar A, et al. <a href="https://jove.unicartagenaproxy.elogim.com/v/62734">Isolated Lung Perfusion System in the Rabbit Model.</a> J. Vis. Exp. (2021).
This video demonstrates the surgical creation of an opening in the rabbit trachea to insert a tracheal cannula for artificially ventilating the lungs.

Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea

Source: Pacheco-Baltazar A, et al. Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021).
This video demonstrates the surgical creation ...

Tracheal cannulation is a surgical procedure that involves creating an opening through the neck into the trachea, followed by the insertion of a cannula or a thin hollow tube to facilitate mechanical ventilation of the lungs.
For tracheal cannulation in a rabbit, begin by injecting a healthy rabbit with a suitable sedative. Place the anesthetized rabbit in a supine position on an operating table. Now, shave the rabbit&#39;s torso region to reveal the underlying skin. Place a ventilation system behind the rabbit&#39;s head near the operating table. This enables quick cannulation after tracheal incision to prevent any tissular damage.
Next, perform a midline incision from the neck to the diaphragm to reveal the trachea. Carefully incise the anterior tracheal region between the cartilage rings to create an opening. Now, insert a cannula of appropriate dimension through the tracheal fibrous membrane, a tissue layer covering the trachea. Pass a ligature around the inserted cannula and tighten the suture to fix it.
Place a spacer underneath the trachea to ensure the cannula does not bend and obstruct the airflow. The tracheal cannula is now ready for connection to the ventilation system to initiate oxygenation of the lungs.

tracheal-cannulation-rabbit-model-procedure-to-insert-tracheal

Universidad de Cartagena UDEC

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JoVE Encyclopedia of Experiments

Biology

Large Animal Models

Leporine

1.7K 次观看. 来源:Pacheco-Baltazar A 等人 兔模型中的离体肺灌注系统。J. Vis. Exp. （2021）。 该视频演示了在兔气管中手术创建一个开口，以插入气管插管，用于人工通气肺部。 

视频: 兔模型气管插管:将气管插管插入兔气管的程序 - 概念

Perfusion and Inflation of the Mouse Lung for Tumor Histology

The ability to evaluate lung histology is critical for the fields of lung cancer research and cancer metastasis. It is equally important to perform necropsies rapidly and efficiently from studies without sacrificing the quality of the tissues procured. The goal of this protocol is to present a method to rapidly perfuse, inflate, and fix mouse lungs for downstream histological analysis. This method does not standardize lung inflation; thus, it does not require any special procedures or equipment and instead simply instills fixative directly through the trachea following perfusion through the heart. This allows for sufficient estimation of tumor size, histology, and scoring. This also allows for the collection of frozen tissue prior to lung tissue fixation. This method is limited in that it does not allow for later morphometric quantification of the lung; however, it is more than sufficient for lung tumor analysis from genetically engineered mouse models (GEMMs), syngeneic models, as well as xenograft tumor and metastasis studies.

The purpose of this method is to present a simple and efficient method for the perfusion, inflation, and fixation of mouse lungs for the examination of lung tumor pathology and evaluation of metastases to the lung.

肿瘤病理学鼠肺的灌注和膨胀

The ability to evaluate lung histology is critical for the fields of lung cancer research and cancer metastasis. It is equally important to perform ...

科研

JoVE Journal

癌症研究

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. As this technology expands and improves, the ability to potentially evaluate and improve the quality of substandard lungs prior to transplant is a critical need. In order to more rigorously evaluate these approaches, a reproducible animal model needs to be established that would allow for testing of improved techniques and management of the donated lungs as well as to the lung-transplant recipient. In addition, an EVLP animal model of associated pathologies, e.g., ventilation induced lung injury (VILI), would provide a novel method to evaluate treatments for these pathologies. Here, we describe the development of a rat EVLP lung program and refinements to this method that allow for a reproducible model for future expansion. We also describe the application of this EVLP system to model VILI in rat lungs. The goal is to provide the research community with key information and &#8220;pearls of wisdom&#8221;/techniques that arose from trial and error and are critical to establishing an EVLP system that is robust and reproducible.

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.

的隔离方法<em&gt;离体</em&gt;肺灌注的大鼠模型:教训制定鼠EVLP课程中学

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

医学

Air-Inflation of Murine Lungs with Vascular Perfusion-Fixation

Lung histology is often used to investigate the contributions provided by airspace cells during lung homeostasis and disease pathogenesis. However, commonly used instillation-based fixation methods can displace airspace cells and mucus into terminal airways and can alter tissue morphology. In comparison, vascular perfusion-fixation techniques are superior at preserving the location and morphology of cells within airspaces and the mucosal lining. However, if positive airway pressure is not simultaneously applied, regions of the lungs may collapse and capillaries may bulge into the alveolar spaces, leading to distortion of the lung anatomy. Herein, we describe an inexpensive method for air-inflation during vascular perfusion-fixation to preserve the morphology and location of airway and alveolar cells and interstitium in murine lungs for downstream histologic studies. Constant air pressure is delivered to the lungs via the trachea from a sealed, air-filled chamber that maintains pressure via an adjustable liquid column while fixative is perfused through the right ventricle.

Presented is a method for air-inflation with vascular perfusion-fixation of the lungs that preserves the location of cells within airways, alveoli and interstitium for structure-function analyses. Constant airway pressure is maintained with an air-inflation chamber while fixative is perfused via the right ventricle. Lungs are processed for histologic studies.

血管灌注固定的穆林肺的空气膨胀

Lung histology is often used to investigate the contributions provided by airspace cells during lung homeostasis and disease pathogenesis. However, ...

Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea

成績單

Tracheal Cannulation in Rabbit Model: A Procedure to Insert Tracheal Cannula into the Rabbit Trachea