Surgical Implantation of Temperature Telemetric Monitoring System in Mouse Model: A Surgical Procedure to Implant Temperature Telemetric Device to Measure Mouse Core Body Temperature

Telemetry facilitates the continuous real-time measurement of physiological signals via a wireless implanted telemetry device in a freely moving live animal.

To implant a temperature telemetric device, first, prep an anesthetized mouse in the supine position. Next, make a midline incision on the abdominal skin. Separate the skin from the underlying muscle layer.

Now, make a small incision on the linea alba - a thin band of connective tissue that separates the abdominal muscles - to access the intraperitoneal cavity. Through the opened muscle layer, gently transfer the sterile temperature battery-free telemetry device into the intraperitoneal cavity.

Close the surgical incision. The implanted telemetry device floats in the cavity and eliminates excess tension on the abdominal wall. Return the mouse to its cage placed on a telemetry receiver platform.

The telemetry receiver powers the implanted telemetry device. The device continuously measures the mouse's core body temperature and transmits the physiological data through radio waves detected by the receiver.

The receiver transforms this radio signal into a digital signal, which is relayed to the computer-based data acquisition system. The acquisition system converts the signal into the desired format and stores the information for subsequent analysis.