The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University

1 ARTICLES PUBLISHED IN JoVE

Medicine

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

¹The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University

This protocol describes a method for screening high-quality sperm with a low DNA fragmentation index by utilizing sperm motility characteristics and thigmotaxis. The method employs a U-shaped horizontal swimming lane, enabling high-quality sperm to reach the opposite side through buffer droplets, while dead sperm, cell debris, and impurities are excluded.