Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
8.7K Views
•
09:17 min
•
July 12th, 2019
DOI :
July 12th, 2019
•Lindsay A. Dawson1, Regina Brunauer1, Katherine N. Zimmel1, Osama Qureshi1, Alyssa R. Falck2, Patrick Kim3, Connor P. Dolan1, Ling Yu1, Yu-Lieh Lin1, Benjamin Daniel1, Mingquan Yan1, Ken Muneoka1
1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 2Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 3Department of Neurosurgery, University of Mississippi Medical Center
Chapters in this video
0:04
Title
0:47
Adult Mouse Hind Limb Distal P3 Amputation
2:01
Digit Collection and Tissue Preparation
2:47
Immunostaining for Blastema Formation and Intramembranous Ossification
4:30
Sequential In Vivo Microcomputed Tomography (microCT) Imaging
5:50
Reconstructed Bone Image Analysis
8:15
Results: Representative Regenerative Bone Analyses
8:47
Conclusion
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