CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
9.4K Views
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09:39 min
•
June 9th, 2018
DOI :
June 9th, 2018
•Weijun Feng*1, Lena Herbst*2, Peter Lichter2, Stefan M. Pfister3,4,5, Hai-Kun Liu1, Daisuke Kawauchi3,4
1Division of Molecular Neurogenetics, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, 2Division of Molecular Genetics, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 3Hopp-Children's Cancer Center at the NCT Heidelberg (KiTZ), 4Division of Pediatric Neurooncology, German Cancer Research Center (DKFZ), and German Cancer Consortium (DKTK), 5Department of Pediatric Hematology and Oncology, Heidelberg University Hospital
Chapters in this video
0:04
Title
1:30
In Utero Electroporation
5:16
Preparation of Cryosections from Electroporated Cerebella
6:22
Immunostaining the Cerebellar Cryosections
7:38
Results: Immunostained Cerebella from Rosa26-CAG-LSL-Cas9-P2A-EGFP Mouse
8:48
Conclusion
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