Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
6.9K Views
•
09:56 min
•
February 18th, 2017
DOI :
February 18th, 2017
•Chapters in this video
0:05
Title
0:51
Transfer of hiPSCs Grown on iMEF Feeder Cells onto Extracellular Matrix for Propagation
2:56
Small-scale Passaging of hiPSCs on Matrix-coated Plates
4:05
Preparation of Chamber Slides and Coverslips for Seeding of hiPSCs for Immunocytochemistry
6:04
Immunostaining of hiPSCs with Pluripotency Markers
8:12
Results: Representative Images of iPSC Colonies and Immunocytochemical Characterization of Human iPSCs
9:10
Conclusion
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