وأيد هذا البحث من المعاهد الوطنية للصحة منح R01HL086939 (NCC).

<ol>
	<li>Pace, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sympathetic+control+of+pulmonary+vascular+impedance+in+anesthetized+dogs.">Sympathetic control of pulmonary vascular impedance in anesthetized dogs.</a> Circ Res. 29, 555-568 (1971).</li><li>Ewalenko, P., Stefanidis, C., Holoye, A., Brimioulle, S., Naeije, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+vascular+impedance+vs.+resistance+in+hypoxic+and+hyperoxic+dogs:+effects+of+propofol+and+isoflurane.">Pulmonary vascular impedance vs. resistance in hypoxic and hyperoxic dogs: effects of propofol and isoflurane.</a> J Appl Physiol. 74, 2188-2193 (1993).</li><li>Nichols, W. W., O'Rourke, M. F., Hartley, C., McDonald, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> McDonald's blood flow in arteries : theoretical, experimental, and clinical principles. , (2005).</li><li>Tuchscherer, H. A., Webster, E. B., Chesler, N. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+Vascular+Resistance+and+Impedance+in+Isolated+Mouse+Lungs:+Effects+of+Pulmonary+Emboli.">Pulmonary Vascular Resistance and Impedance in Isolated Mouse Lungs: Effects of Pulmonary Emboli.</a> Annals of Biomedical Engineering. 34, 660-668 (2006).</li><li>Tuchscherer, H. A., Vanderpool, R. R., Chesler, N. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulmonary+vascular+remodeling+in+isolated+mouse+lungs:+Effects+on+pulsatile+pressure-flow+relationships.">Pulmonary vascular remodeling in isolated mouse lungs: Effects on pulsatile pressure-flow relationships.</a> Journal of Biomechanics. 40, 993-1001 (2007).</li><li>Vanderpool, R., Naeije, R., Chesler, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impedance+in+Isolated+Mouse+Lungs+for+the+Determination+of+Site+of+Action+of+Vasoactive+Agents+and+Disease.">Impedance in Isolated Mouse Lungs for the Determination of Site of Action of Vasoactive Agents and Disease.</a> Ann Biomed Eng. , (2010).</li><li>Vanderpool, R., Kim, A., Molthen, R., Chesler, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+acute+rho+kinase+inhibition+on+chronic+hypoxia-induced+changes+in+proximal+and+distal+pulmonary+arterial+structure+and+function.">Effects of acute rho kinase inhibition on chronic hypoxia-induced changes in proximal and distal pulmonary arterial structure and function.</a> Journal of Applied Physiology. , (2010).</li><li>El-Bizri, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Smooth+muscle+protein+22alpha-mediated+patchy+deletion+of+Bmpr1a+impairs+cardiac+contractility+but+protects+against+pulmonary+vascular+remodeling.">Smooth muscle protein 22alpha-mediated patchy deletion of Bmpr1a impairs cardiac contractility but protects against pulmonary vascular remodeling.</a> Circ Res. 102, 380-388 (2008).</li><li>Champion, H., Michelakis, E., Hassoun, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comprehensive+invasive+and+noninvasive+approach+to+the+right+ventricle-pulmonary+circulation+unit:+state+of+the+art+and+clinical+and+research+implications.">Comprehensive invasive and noninvasive approach to the right ventricle-pulmonary circulation unit: state of the art and clinical and research implications.</a> Circulation. 120, 992-1007 (2009).</li><li>Gan, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasively+assessed+pulmonary+artery+stiffness+predicts+mortality+in+pulmonary+arterial+hypertension.">Noninvasively assessed pulmonary artery stiffness predicts mortality in pulmonary arterial hypertension.</a> Chest. 132, 1906-1912 (2007).</li><li>Mahapatra, S., Nishimura, R., Sorajja, P., Cha, S., McGoon, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Relationship+of+pulmonary+arterial+capacitance+and+mortality+in+idiopathic+pulmonary+arterial+hypertension.">Relationship of pulmonary arterial capacitance and mortality in idiopathic pulmonary arterial hypertension.</a> J Am Coll Cardiol. 47, 799-803 (2006).</li><li>Bogaard, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+pulmonary+artery+pressure+elevation+is+insufficient+to+explain+right+heart+failure.">Chronic pulmonary artery pressure elevation is insufficient to explain right heart failure.</a> Circulation. 120, 1951-1960 (2009).</li></ol>

الإعلان عن أي تضارب في المصالح.

خطوات حاسمة في الجراحة فمن الأهمية بمكان أن يتم أخذ الحذر عند قطع القفص الصدري بعيدا عن الرئتين. يجب أن تكون عرضة الرئتين تماما وغير مطروقة من قبل الأنسجة المحيطة بها ولكن ليس خلال التضخم التي تضررت خلال عملية العزل. ويمكن استخدام ?...

<table><tbody><tr><td>1 ml syringe</td><td>Fisher Scientific</td><td>14-829-10F</td><td></td></tr><tr><td>10 ml syringe</td><td>Fisher Scientific</td><td>14-823-2A</td><td></td></tr><tr><td>60 ml syringe</td><td>Fisher Scientific</td><td>13-689-8</td><td></td></tr><tr><td>RPMI with GLN 6/PK</td><td>Fisher Scientific</td><td>MT10040CV</td><td></td></tr><tr><td>Bottle Top Filters</td><td>Fisher Scientific</td><td>09-761-57</td><td></td></tr><tr><td>Ficoll PM 70</td><td>Sigma-Aldrich</td><td>F2878-100g</td><td></td></tr><tr><td>Heparin</td><td>Sigma-Aldrich</td><td></td><td></td></tr><tr><td>Y27632</td><td>Sigma-Aldrich</td><td>Y0503</td><td></td></tr><tr><td>Angled Ball Iris scissors</td><td>Fine Science Tools</td><td>14109-09</td><td></td></tr><tr><td>Vannas Spring Scissors - 4mm Blades</td><td>Fine Science Tools</td><td>15018-10</td><td></td></tr><tr><td>Fine Iris Scissors - straight</td><td>Fine Science Tools</td><td>14106-09</td><td></td></tr><tr><td>Dumont #5/45 Forceps</td><td>Fine Science Tools</td><td>11251-35</td><td></td></tr><tr><td>Dumont Medical Biology Forceps</td><td>Fine Science Tools</td><td>11254-20</td><td></td></tr><tr><td>Lauda E100 ECO-line 003 </td><td>VWR international</td><td>Comparable to Lauda-Brinkmann E-103, 62400-922</td><td></td></tr><tr><td>IL-1 Isolated perfused mouse lung system </td><td>Harvard Apparatus</td><td>739904</td><td></td></tr><tr><td>Blood Pressure Transducer P75 for PLUGSYS Module</td><td>Harvard Apparatus</td><td>730020</td><td></td></tr><tr><td>TS410 Flow Modules</td><td>Transonic</td><td>TS410</td><td></td></tr><tr><td>ME 4 PXN Precision PXN Inline Flowsensors</td><td>Transonic</td><td>ME 4 PXN</td><td></td></tr><tr><td>Cole-Parmer Multi-Syringe Pumps</td><td>Cole-Parmer</td><td>EW-74900-20</td><td></td></tr><tr><td>Nembutal 50MG/ML 20ML Vial</td><td>Amatheon</td><td></td><td></td></tr></tbody></table>

characterization of the isolated, ventilated, and instrumented mouse lung perfused with pulsatile flow

المعزولة ، التهوية والمجهزة إعداد الرئة الماوس يسمح بقياس الأوعية الدموية الرئوية مطرد ونابض ضغط التدفق العلاقات مع مستقلة السيطرة على معدل التدفق الشرياني الرئوي ، وتدفق معدل الموجي ، ضغط الهواء وضغط الأذين الأيسر. يحسب المقاومة الوعائية الرئوية على أساس متعدد النقطة ، منحنيات ضغط التدفق المطرد ؛ يحسب مقاومة الأوعية الدموية الرئوية من منحنيات ضغط تدفق نابض الحصول على مجموعة من الترددات. كما يعترف الآن سريريا ، هو قياس المعاوقة متفوقة من حمولة تلوية البطين الأيمن من المقاومة لأنها تضم ​​تأثيرات الامتثال الأوعية الدموية ، والتي لا يكاد يذكر ، وخصوصا في الدورة الدموية الرئوية. ثلاثة قياسات مهمة للمقاومة -- صفر هيرتز المعاوقة Z 0 ، خاصية مقاومة Z C ، ومؤشر انعكاس موجة R ث -- توفير نظرة ثاقبة القاصي منطقة مستعرضة الشرياني المتاحة للتدفق ، وتصلب الشرايين ومقاومة الداني في المنبع والمصب فمنها ، على التوالي. جميع النتائج التي تم الحصول عليها في عزلة ، وتهوية الرئتين perfused تكون مستقلة من المتعاطفين نغمة الجهاز العصبي ، والحالة حجم وآثار التخدير. وقد استخدمنا هذه التقنية لقياس أثر الصمات الرئوية المزمنة ونقص الأكسجين على المقاومة والممانعة ، والتفريق بين مواقع العمل (أي مقابل الداني القاصي) من وكلاء فعال في الأوعية والأمراض باستخدام التبعية ضغط Z C. وعلاوة على ذلك ، وعندما تستخدم هذه التقنيات مع الرئتين من سلالات من الفئران المعدلة وراثيا ، يمكن تحديد الآثار المترتبة على المستوى الجزيئي عيوب الأوعية الدموية الرئوية في الهيكل والوظيفة.

Watch this Scientific Journal Video about Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow at JoVE.com

Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

بروتوكول التالية توضح عملية عزل والتهوية والرئتين instrumenting الماوس لقياس الأوعية الدموية الرئوية الثابتة أو نابض ضغط التدفق العلاقات من أجل تحديد الآثار المترتبة على تدفق الدم ، وتدفق الهواء ، والتغيرات الهوائية والأوعية الدموية التغييرات على حمولة تلوية البطين الأيمن.

توصيف المعزولة ، مهواة ، والرئة ماوس المجهزة Perfused مع تدفق نابض

The isolated, ventilated and instrumented mouse lung preparation allows steady and pulsatile pulmonary vascular pressure-flow relationships to be measured ...

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16.2K Views.  University of Wisconsin – Madison. بروتوكول التالية توضح عملية عزل والتهوية والرئتين instrumenting الماوس لقياس الأوعية الدموية الرئوية الثابتة أو نابض ضغط التدفق العلاقات من أجل تحديد الآثار المترتبة على تدفق الدم ، وتدفق الهواء ، والتغيرات الهوائية والأوعية الدموية التغييرات على حمولة تلوية البطي...

Video: Characterization of the Isolated, Ventilated, and Instrumented Mouse Lung Perfused with Pulsatile Flow

Perfusion and Inflation of the Mouse Lung for Tumor Histology

The ability to evaluate lung histology is critical for the fields of lung cancer research and cancer metastasis. It is equally important to perform necropsies rapidly and efficiently from studies without sacrificing the quality of the tissues procured. The goal of this protocol is to present a method to rapidly perfuse, inflate, and fix mouse lungs for downstream histological analysis. This method does not standardize lung inflation; thus, it does not require any special procedures or equipment and instead simply instills fixative directly through the trachea following perfusion through the heart. This allows for sufficient estimation of tumor size, histology, and scoring. This also allows for the collection of frozen tissue prior to lung tissue fixation. This method is limited in that it does not allow for later morphometric quantification of the lung; however, it is more than sufficient for lung tumor analysis from genetically engineered mouse models (GEMMs), syngeneic models, as well as xenograft tumor and metastasis studies.

The purpose of this method is to present a simple and efficient method for the perfusion, inflation, and fixation of mouse lungs for the examination of lung tumor pathology and evaluation of metastases to the lung.

التخبط والتضخم في الرئة الماوس لعلم الأنسجة الورم

The ability to evaluate lung histology is critical for the fields of lung cancer research and cancer metastasis. It is equally important to perform ...

Cancer Research

Videomorphometric Analysis of Hypoxic Pulmonary Vasoconstriction of Intra-pulmonary Arteries Using Murine Precision Cut Lung Slices

Acute alveolar hypoxia causes pulmonary vasoconstriction (HPV) - also known as von Euler-Liljestrand mechanism - which serves to match lung perfusion to ventilation. Up to now, the underlying mechanisms are not fully understood. The major vascular segment contributing to HPV is the intra-acinar artery. This vessel section is responsible for the blood supply of an individual acinus, which is defined as the portion of lung distal to a terminal bronchiole. Intra-acinar arteries are mostly located in that part of the lung that cannot be selectively reached by a number of commonly used techniques such as measurement of the pulmonary artery pressure in isolated perfused lungs or force recordings from dissected proximal pulmonary artery segments1,2. The analysis of subpleural vessels by real-time confocal laser scanning luminescence microscopy is limited to vessels with up to 50 &#181;m in diameter3.
We provide a technique to study HPV of murine intra-pulmonary arteries in the range of 20-100 &#181;m inner diameters. It is based on the videomorphometric analysis of cross-sectioned arteries in precision cut lung slices (PCLS). This method allows the quantitative measurement of vasoreactivity of small intra-acinar arteries with inner diameter between 20-40 &#181;m which are located at gussets of alveolar septa next to alveolar ducts and of larger pre-acinar arteries with inner diameters between 40-100 &#181;m which run adjacent to bronchi and bronchioles. In contrast to real-time imaging of subpleural vessels in anesthetized and ventilated mice, videomorphometric analysis of PCLS occurs under conditions free of shear stress. In our experimental model both arterial segments exhibit a monophasic HPV when exposed to medium gassed with 1% O2 and the response fades after 30-40 min at hypoxia.

Hypoxic pulmonary vasoconstriction (HPV) is an important physiological phenomenon by which at alveolar hypoxia lung perfusion is matched to ventilation. The major vascular segment contributing to HPV is the intra-acinar artery. Here, we describe our protocol for the analysis of HPV of murine pulmonary vessels with diameters of 20-100 &mu;m.

تحليل مجسم الفيديو لنقص الأكسجين الرئوي من الشرايين داخل الرئة باستخدام مورين الدقة قطع شرائح الرئة

Acute alveolar hypoxia causes pulmonary vasoconstriction (HPV) - also known as von Euler-Liljestrand mechanism - which serves to match lung perfusion to ...

Medicine

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. As this technology expands and improves, the ability to potentially evaluate and improve the quality of substandard lungs prior to transplant is a critical need. In order to more rigorously evaluate these approaches, a reproducible animal model needs to be established that would allow for testing of improved techniques and management of the donated lungs as well as to the lung-transplant recipient. In addition, an EVLP animal model of associated pathologies, e.g., ventilation induced lung injury (VILI), would provide a novel method to evaluate treatments for these pathologies. Here, we describe the development of a rat EVLP lung program and refinements to this method that allow for a reproducible model for future expansion. We also describe the application of this EVLP system to model VILI in rat lungs. The goal is to provide the research community with key information and &#8220;pearls of wisdom&#8221;/techniques that arose from trial and error and are critical to establishing an EVLP system that is robust and reproducible.

Method of Isolated Ex Vivo Lung Perfusion in a Rat Model: Lessons Learned from Developing a Rat EVLP Program

Ex-Vivo Lung Perfusion (EVLP) has allowed lung transplantation in humans to become more readily available by enabling the ability to assess organs and expand the donor pool. Here, we describe the development of a rat EVLP program and refinements that allow for a reproducible model for future expansion.

طريقة معزولة<em&gt; فيفو السابقين</em&gt; الإرواء الرئة في الفئران نموذج: الدروس المستفادة من تطوير برنامج EVLP الجرذ

The number of acceptable donor lungs available for lung transplantation is severely limited due to poor quality. Ex-Vivo Lung Perfusion (EVLP) has allowed ...

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

The isolated blood-perfused lung preparation is widely used to visualize and define signaling in single microvessels. By coupling this preparation with real time imaging, it becomes feasible to determine permeability changes in individual pulmonary microvessels. Herein we describe steps to isolate rat lungs and perfuse them with autologous blood. Then, we outline steps to infuse fluorophores or agents via a microcatheter into a small lung region. Using these procedures described, we determined permeability increases in rat lung microvessels in response to infusions of bacterial lipopolysaccharide. The data revealed that lipopolysaccharide increased fluid leak across both venular and capillary microvessel segments. Thus, this method makes it possible to compare permeability responses among vascular segments and thus, define any heterogeneity in the response. While commonly used methods to define lung permeability require postprocessing of lung tissue samples, the use of real time imaging obviates this requirement as evident from the present method. Thus, the isolated lung preparation combined with real time imaging offers several advantages over traditional methods to determine lung microvascular permeability, yet is a straightforward method to develop and implement.

The isolated blood-perfused lung preparation makes it feasible to visualize microvessel networks on the lung surface. Here we describe an approach to quantify permeability of single microvessels in isolated lungs using real time fluorescence imaging.

قياس النفاذية في واحدة Microvessel المعزولة الجرذ إعداد الرئة perfused الدم

The isolated blood-perfused lung preparation is widely used to visualize and define signaling in single microvessels. By coupling this preparation with ...

Isolated Lung Perfusion System in the Rabbit Model

The isolated lung perfusion system has been widely used in pulmonary research, contributing to elucidate the lungs&#39; inner workings, both micro and macroscopically. This technique is useful in the characterization of pulmonary physiology and pathology by measuring metabolic activities and respiratory functions, including interactions between circulatory substances and the effects of inhaled or perfused substances, as in drug testing. While in vitro methods involve the slicing and culturing of tissues, the isolated ex vivo lung perfusion system allows to work with a complete functional organ making possible the study of a continuous physiological function while recreating ventilation and perfusion. However, it should be noted that the effects of the absence of central innervation and lymphatic drainage still have to be fully assessed. This protocol aims to describe the assembly of the isolated lung apparatus, followed by the surgical extraction and cannulation of lungs and heart from experimental lab animals, as well as to display the perfusion technique and signal processing of data. The average viability of the isolated lung ranges between 5-8 h; during this period, the pulmonary capillary permeability increases, causing edema and lung injury. The functionality of preserved pulmonary tissue is measured by the capillary filtration coefficient (Kfc), used to determine the extent of pulmonary edema through time.

The isolated rabbit lung preparation is a gold standard tool in pulmonary research. This publication aims to describe the technique as developed for the study of physiological and pathological mechanisms involved in airway reactivity, lung preservation, and preclinical research in lung transplantation and pulmonary edema.

عزل الرئة Perfusion النظام في نموذج الأرنب

The isolated lung perfusion system has been widely used in pulmonary research, contributing to elucidate the lungs&#39; inner workings, both micro and ...

Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation

Since the establishment of lung transplantation as a therapeutic strategy for advanced lung diseases, the scientific community is faced with the problem of a low number of lungs considered viable for the donation process. In recent decades, however, this scenario has been positively changed, given the development of ex vivo lung perfusion (EVLP) as a strategy for evaluating and reconditioning marginal lungs. The establishment of EVLP in large transplant centers has favored an increase in the number of lung transplants, both by increasing the diagnostic accuracy of lung function and by constituting an effective platform for the reconditioning of lung grafts. In this context, faced with ethical and logistical issues, as well as in the study of immunological factors associated with lung transplantation, the development of rodent EVLP models has become important, given their reliability, the possibility of genetic manipulation, and lower costs. This paper describes a protocol for establishing a rat EVLP model and shows the inflammatory profile associated with the perfused lungs. This will help propagate knowledge about the rat EVLP model, promoting our understanding of the biological responses associated with that revolutionary technique.

Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation

Here, we describe the necessary steps for establishing a rat EVLP model and show the inflammatory profile associated with the perfused lungs. The aim is to propagate knowledge and experiences about the rat EVLP model, enabling the integral understanding of the biological responses associated with that revolutionary technique.

Since the establishment of lung transplantation as a therapeutic strategy for advanced lung diseases, the scientific community is faced with the problem ...

Evaluation of Apoptosis and Lung Injury in DCD Donor Lungs Preserved by EVLP

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death

The increasing scarcity of donor lungs for transplantation has spurred significant interest in the utilization of organs procured from donors after cardiac death (DCD). However, a critical challenge remains in maximizing the number of viable lungs that meet stringent criteria for clinical use. Ex vivo lung perfusion (EVLP) emerges as a promising technology to evaluate and potentially improve the function of donated lungs. This paper describes the development of a cost-effective and reproducible small animal experimental platform specifically designed for EVLP research. This platform offers a high degree of customization, enabling researchers to tailor experimental parameters to address specific research questions within the field of EVLP. The team established a rat model to simulate DCD and implemented a two-stage preservation approach. Lungs were subjected to static cold storage using a perfusate solution at 4 &#176;C for 4 h, followed by normothermic EVLP for another 4 h. Researchers evaluated various lung function parameters, including pulmonary vascular resistance, pulmonary dynamic compliance, blood gas measurements of the perfusate, and apoptotic cell death in lung tissues. The study demonstrates that EVLP could significantly improve the functional performance of DCD lungs compared to simple cold storage at 4 &#176;C, suggesting that EVLP has the potential to mitigate ischemia-reperfusion injury and enhance graft function following circulatory death, thereby increasing the pool of transplantable lungs.

The objective is to develop a new ex vivo lung perfusion (EVLP) model in rats that can sustain consistent lung function for 4 h throughout EVLP and furnish the industry with implementation specifics that will offer a sturdy method for applying EVLP to rodent models.

The increasing scarcity of donor lungs for transplantation has spurred significant interest in the utilization of organs procured from donors after ...

Design and Implementation of a Rat Ex Vivo Lung Perfusion Model

Ex vivo lung preparations are a useful model that can be translated to many different fields of research, complementing corresponding in vivo and in vitro models. Laboratories wishing to use isolated lungs need to be aware of important steps and inherent challenges to establish a setup that is affordable, reliable, and that can be easily adapted to fit the topic of interest. This paper describes a DIY (do it yourself) model for ex vivo rat lung ventilation and perfusion to study drug and gas effects on pulmonary vascular tone, independent of changes in cardiac output. Creating this model includes a) the design and construction of the apparatus, and b) the lung isolation procedure. This model results in a setup that is more cost-effective than commercial alternatives and yet modular enough to adapt to changes in specific research questions. Various obstacles had to be resolved to ensure a consistent model that is capable of being used for a variety of different research topics. Once established, this model has proven to be highly adaptable to different questions and can easily be altered for different fields of study.

Design and Implementation of a Rat Ex Vivo Lung Perfusion Model

Ex vivo lungs are useful for a variety of experiments to collect physiological data while excluding the confounding variables of in vivo experiments. Commercial setups are often expensive and limited in the types of data they can collect. We describe a method for building a fully modular setup, adaptable for various study designs.

تصميم وتنفيذ نموذج تروية الرئة خارج الجسم الحي للفئران

Ex vivo lung preparations are a useful model that can be translated to many different fields of research, complementing corresponding in vivo and in vitro ...

Mouse Lung Preparation for Flow Cytometry: Generating Cell Suspension from Lung Tissue for Flow Cytometric Analysis

Source: Moll, H. P., et al. <a href="https://jove.unicartagenaproxy.elogim.com/v/58101/orthotopic-transplantation-syngeneic-lung-adenocarcinoma-cells-to">Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression.</a> J. Vis. Exp. (2019).
This video describes the preparation of mouse lung cell suspension for flow cytometric analysis to study PD-L1 expression of lung adenocarcinoma cells.

تحضير رئة الفأر لقياس التدفق الخلوي: توليد تعليق الخلية من أنسجة الرئة لتحليل قياس التدفق الخلوي

Source: Moll, H. P., et al. Orthotopic Transplantation of Syngeneic Lung Adenocarcinoma Cells to Study PD-L1 Expression. J. Vis. Exp. (2019).
This video ...

JoVE Encyclopedia of Experiments

Lung Cancer

Ex vivo study

Ex vivo Lung Perfusion: A Technique for Pulmonary Research in a Rabbit Model

Source: Pacheco-Baltazar, A., et al., <a href="https://jove.unicartagenaproxy.elogim.com/v/62734">Isolated Lung Perfusion System in the Rabbit Model.</a> J. Vis. Exp. (2021).
This video shows an ex vivo technique for isolated lung perfusion. It is a tool for pulmonary research that will aid studies of various physiological and pathological mechanisms involved in lung transplantation procedures.

نضح الرئة خارج الجسم الحي: تقنية للأبحاث الرئوية في نموذج الأرانب

Source: Pacheco-Baltazar, A., et al., Isolated Lung Perfusion System in the Rabbit Model. J. Vis. Exp. (2021).
This video shows an ex vivo technique for ...

توصيف المعزولة ، مهواة ، والرئة ماوس المجهزة Perfused مع تدفق نابض

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التخبط والتضخم في الرئة الماوس لعلم الأنسجة الورم

تحليل مجسم الفيديو لنقص الأكسجين الرئوي من الشرايين داخل الرئة باستخدام مورين الدقة قطع شرائح الرئة

طريقة معزولة

قياس النفاذية في واحدة Microvessel المعزولة الجرذ إعداد الرئة perfused الدم

عزل الرئة Perfusion النظام في نموذج الأرنب

Establishment of an Ex Vivo Lung Perfusion Rat Model for Translational Insights in Lung Transplantation

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death

تصميم وتنفيذ نموذج تروية الرئة خارج الجسم الحي للفئران

تحضير رئة الفأر لقياس التدفق الخلوي: توليد تعليق الخلية من أنسجة الرئة لتحليل قياس التدفق الخلوي

نضح الرئة خارج الجسم الحي: تقنية للأبحاث الرئوية في نموذج الأرانب