This study was funded by the Chilean projects ANID / Fondecyt / Regular 1201311 and 1241091. The authors would like to thank the enterprise BleuSolid for its support during the latest HMI improvements,&#160;and Geropolis for the filming room.

&#8203;The study was approved by the Ethical Committee of Universidad de Valparaiso, Chile, under protocol number CEC213-20. Written informed consent was provided for the participants. A telephone interview was carried out to establish the inclusion/exclusion of the participants. The study has been registered under the following reference: NCT05424536.
1. Device set up
<ol>
	<li>Place the device&#39;s main parts on a large table.
	<ol>
		<li>Place side by side the following parts: electric insulation transformer, electronic module and laptop computer on a large table. Ensure there is sufficient space in front of these parts in order to place the participant&#39;s forearm with ease later.</li>
		<li>Eventually, place the laptop computer directly on the electronic module in case of limited space, taking care not to obstruct the ventilation windows of the module, which are clearly indicated.</li>
	</ol>
	</li>
	<li>Connect the electric insulation transformer.
	<ol>
		<li>Plug the electric insulation transformer into the room&#39;s domestic power using a dedicated cable.</li>
	</ol>
	</li>
	<li>Connect the electronic module.
	<ol>
		<li>Connect the electronic module to the electric insulation transformer using a dedicated power cable.</li>
		<li>Press the ON-OFF button on the transformer to power the module.</li>
	</ol>
	</li>
	<li>Connect the laptop computer.
	<ol>
		<li>Connect the laptop computer to the module using the dedicated universal serial bus (USB) cable to send the digitized received signals to the computer for further processing.</li>
		<li>If the laptop needs to be powered, connect its power cable to the electronic insulation transformer.</li>
	</ol>
	</li>
	<li>Connect the ultrasonic probe.
	<ol>
		<li>Connect the ultrasonic probe to the module with the dedicated cable slot situated on the front side of the module. There are two different probes for forearm and leg measurement sites. In this study, only the radius (forearm probe) is considered.</li>
	</ol>
	</li>
	<li>Connect the pedal switch.
	<ol>
		<li>Place the pedal switch on the floor close to the feet, considering the operator&#39;s position while measuring a participant. Connect the pedal switch to the computer using a USB cable. Use the pedal to start the acquisition series.</li>
	</ol>
	</li>
</ol>
2. Participant installation
<ol>
	<li>Position the participant.
	<ol>
		<li>Invite the participant to sit in front of the operator with his naked forearm resting on the table in front of the device previously installed (see Figure 3). 
		NOTE: The contralateral side is measured (i.e., the left side for a right-handed participant).</li>
	</ol>
	</li>
	<li>Mark the measurement site (one-third distal radius).
	<ol>
		<li>Measure the radius length using the ruler from the radial styloid (bone end close to the wrist) to the elbow.</li>
		<li>Divide this length by three.</li>
		<li>Mark the measurement site, i.e., one-third distal radius, using the pen by measuring one radius length third from the wrist.</li>
	</ol>
	</li>
	<li>Start the HMI software.
	<ol>
		<li>Start the HMI software by clicking on the corresponding icon on the laptop desktop.</li>
	</ol>
	</li>
	<li>Add the participant&#39;s data.
	<ol>
		<li>Add the participant&#39;s data (anonymized ID, laterality, measured site, operator&#39;s ID, gender, etc.) using the pop-up window, which automatically opens when the software starts.</li>
	</ol>
	</li>
	<li>Add ecographic gel.
	<ol>
		<li>Add ecographic gel on the front side of the probe and on the measurement site, marked on the participant&#39;s forearm, to ensure ultrasonic wave propagation.</li>
	</ol>
	</li>
	<li>Put the probe in contact with the forearm.
	<ol>
		<li>Put the probe in contact with the forearm, with the probe center placed on the mark previously done in step 2.2.</li>
	</ol>
	</li>
</ol>
3. Looking for a stable position
NOTE: The HMI displays four parameters of interest: two velocities, vFAS and vA0, and two inverse problem values, cortical thickness (Ct.Th) and cortical porosity (Ct.Po). The HMI also displays four quality parameters denoted alpha, lowk, max, and diff. These parameters are described in detail in the introduction.
<ol>
	<li>Start the real time visualization.
	<ol>
		<li>Start the real time visualization by clicking on the start button on the right bottom side of the software interface. The duration between two successive value displays is about 0.5 s.</li>
	</ol>
	</li>
	<li>Find a stable vFAS value.
	<ol>
		<li>Slowly adjust the probe position while observing the vFAS parameter value displayed in a specific case of the interface. Normal values range from about 3800 m&#8729;s-1 to about 4200 m&#8729;s-1.</li>
		<li>If a stable position is found, ensure that the vFAS variation is less than about 40 m&#8729;s-1 between two successive calculations.</li>
	</ol>
	</li>
	<li>Adjust the bidirectional angle.
	<ol>
		<li>Slowly adjust the probe position while observing the bidirectional value (quality parameter alpha) displayed in a specific case of the interface.</li>
		<li>Adjust the probe position by softly adding pressure on one probe side until the angle absolute value is less than 2&#176; to improve the parallelism between the probe and the bone surface.</li>
	</ol>
	</li>
	<li>Find a stable vA0 value.
	<ol>
		<li>Slowly adjust the probe position while observing the vA0 parameter value displayed in a specific case of the interface. Normal values range from about 1500 m&#8729;s-1 to about 1900 m&#8729;s-1.</li>
		<li>If a stable position is found, ensure that the vA0 variation is less than about 40 m&#8729;s-1 between two successive calculations.</li>
		<li>In case of difficulty, observe the guided wave image spectra displayed on the right column of the interface. Ensure that the upper part of the spectrum appears as a continuous line, whose slope provides the vA0 value.</li>
	</ol>
	</li>
	<li>Observe the inverse problem image.
	<ol>
		<li>Observe the inverse problem image, which appears automatically once the two velocities (vFAS and vA0) and angle values are stabilized.</li>
		<li>Ensure that the image shows at least one maximum, indicated with a clear pixel, and eventually, one or several secondary maxima, indicated with a different color. The three missing quality parameters (max. diff, lowk) are automatically calculated in real time.</li>
	</ol>
	</li>
	<li>Improve the inverse problem image.
	<ol>
		<li>Slowly adjust the probe position while observing the inverse problem image maxima.</li>
		<li>Find the highest possible first maximum and the lowest possible secondary maximum while looking at the corresponding cases of the interface (max and diff values).</li>
		<li>In case of difficulty, observe the guided wave spectrum image displayed on the right column of the interface. Ensure that the lower part of the spectrum appears with a few continuous lines, as long as possible, associated with high phase velocity modes and the parameter quality lowk, as high as possible.</li>
	</ol>
	</li>
	<li>Find a stable position.
	<ol>
		<li>Once an acceptable inverse problem image is found, stabilize the probe position. Ensure that no significant changes of the inverse problem image are seen between two successive calculations.</li>
	</ol>
	</li>
</ol>
4. Data acquisition
<ol>
	<li>Start a series of 10 acquisitions.
	<ol>
		<li>Once a stable position is found, start a series of 10 acquisitions by pressing the pedal switch with the foot.</li>
		<li>Remain as stable as possible during the 10 acquisitions, lasting about 5 s.</li>
	</ol>
	</li>
	<li>Control the quality of the series.
	<ol>
		<li>Look at the means and standard deviations of the parameters of interest, which are automatically calculated and shown in pop-up windows appearing once the series ends.</li>
		<li>If the standard deviations are lower than fixed thresholds, take the series into account. On the contrary, reject the series.</li>
		<li>Answer to the question asked in a second pop-up window, asking if the operator would like to stop or continue with the acquisition series of the same participant.</li>
	</ol>
	</li>
	<li>Reposition the probe.
	<ol>
		<li>Start again the previous steps (from steps 2.1 to 3.2) to find more stable positions and acquire more series of 10 acquisitions. Eventually, if needed, let the participant rest between two repositionings.</li>
		<li>As before, means and standard deviations of the parameters of interest are automatically calculated for each series.</li>
		<li>Look at the result pop up window to check if the last acquired series is kept or rejected. The participant&#39;s measurement finishes when at least four consistent series are registered. Outsider series are automatically rejected.</li>
	</ol>
	</li>
	<li>Obtain the final values. 
	NOTE: The final values of the parameters of interest are automatically obtained, considering the mean of the means obtained with the consistent series.</li>
	<li>Verify the automatic report pdf.
	<ol>
		<li>Verify that the final values are reported in the report pdf automatically and instantly generated once the option stop is chosen in the pop-up window. An example is shown in Figure 4. 
		NOTE: The pdf is located in the same folder as the raw data, which could be reanalyzed later offline.</li>
	</ol>
	</li>
	<li>Verify the second precise report.
	<ol>
		<li>Verify the second precise report generated using the exact waveguide model values for the inverse problem calculation instead of approximated values as in the case of the first automatic report. The second report generation takes less than 5 min. Examples are shown in Figure 5 and Figure 6.</li>
		<li>Verify that the automatic report agrees with the precise report. Remove the series that were not automatically eliminated in order to keep the consistent series.</li>
	</ol>
	</li>
</ol>

Cortical Bone Assessment Using the Ultrasonic BDAT Device

The authors do not declare conflicts of interest.

The critical point for measurement success is the correct probe positioning with respect to the bone. The position with respect to the bone surface was successfully solved by integrating with the guiding interface the bi-directional correction16,17. It was shown that without this correction, a few percentage error can be reached for the vFAS parameters16. This correction was found crucial to accurately discriminate between patients as the whole measurement range (about 3800-4200 m&#8729;s-1) is about 10% of the mean value17, about 4000 m&#8729;s-1. The reproducibility found in this study (standard deviation of 24 m&#8729;s-1) was similar to the previous study17, indicating a standard deviation of 20 m&#8729;s-1 and a coefficient of variation (CV) of about 0.5%. A similar coefficient of variation (0.5 %) was found at a lower frequency, i.e., 0.3 MHz40. Note that the excellent reliability of the vFAS parameter (ICC = 0.91) was found to be higher than the one recently obtained with another AT device in an older population (ICC = 0.77)41. It should be noted that the population in this case was not only older but also all female and some subjects with other clinical signs of increased bone porosity41.
The second challenge is to also position the probe correctly with respect to the bone axis and the two interfaces in order to obtain the guided modes, in particular those of high phase velocity or low wavenumber. These modes are close to resonance and associated with multiple reflection paths. If the alignment is not correct, waves will be scattered outside the spatial measurement range of the probe. On the contrary, if the probe is correctly positioned, these high-phase velocity modes will appear as continuous branches in the ROI3. For the first version of the current HMI, this alignment was solved by looking at the real-time GWSI20. However, this approach was found insufficient to reach a robust measurement: the failure rate was found to be about 20% in the pilot clinical study20. The inclusion of real-time quality parameters allows the failure to decrease to about 10% in a second clinical study23. Then, the inclusion of the inverse problem image in the &#34;real time&#34; HMI allows further improvement, with a current failure rate for radius measurement of about 5%25. Note that the initial failure with the first device measuring only vFAS was found to be about 15%17.
Reliability on cortical thickness was found to be similar to a previous study22. With a previous version of the same prototype, ICC was also found to be close to 0.9, with a standard deviation of about 0.1 mm and a CV of about 3%. However, a clear improvement is observed in cortical porosity: ICC increased from 0.622 to 0.8, and standard deviation decreased from 1.5% to 1%. The most difficult parameter is vA0 due to the proximity of his velocity with the soft tissue velocity, about 1500 m&#8729;s-1. If the probe is correctly aligned, A0 mode appears unique and continuous. On the contrary, it appears discontinuous and/or multiple due to coupling with surrounding soft tissues. This effect is particularly strong for people with small (less than 4 mm) or large (more than 10 mm) soft tissue thickness (denoted ST.Th).
As exposed before, the key point is to find the correct probe position. However, the position should be not only correct but also stable in order to perform mean and standard deviation for a series of 10 acquisitions. In a majority of cases, finding a stable takes less than 1 min, and the complete measurement of a patient would last about 5 min. Even if the standard protocol described in this study is well suited for a majority of patients, some people are more difficult to measure; it is difficult but possible to find correct positions, but almost impossible to find a stable one. In this case, the operator can choose to record longer series, up to 200 acquisitions. The best acquisitions are later determined offline using quality parameters. This filtering should be applied in real time in the future. In practice, the operator records more series and/or acquisitions than the 4 series of 10 acquisitions of the ideal condition protocol. However, the usual measurement time remains about 5 min for one site, forearm or leg. If, after a few minutes, the HMI does not detect any correct position, the measurement stops and is considered a failure. With the current device and protocol, the failure rate has been found to be less than 5%25.
The current device faces different limitations: 
(i)Size and weight: The current BDAT device is portable: it fits in standard luggage and weighs about 25 kg. However, this weight is large with respect to the newest ultrasonic devices. A new electronic design may be considered, with the probe and signal processing remaining the same. However, it is possible to move the current device, particularly for patients in bed or at home with limited mobility.
(ii)Acquisition speed: The current frame rate is about 2-4 Hz, meaning that the exploration of the measurement is slow compared to actual real-time acquisition, i.e., higher than 25 Hz. This could be improved in the future, taking into account faster computers, faster data analysis, and transmission between the electronics and the computer. An increase in the acquisition speed would improve the user-friendliness of the measurement, particularly the search for correct probe positioning.
(iii)Soft tissue thickness: The current approach is limited by a large soft tissue layer, typically superior to 10 mm. In this case, the first arriving signal is linked with the soft tissue path and not the cortical bone. Thus, vFAS and the associated bi-directional angle can not be used. Likewise, vA0 is very difficult to measure for large soft tissue layers. Without these two velocities, the inverse problem cannot be performed. In the future, other methods of bi-directional correction could be applied using imaging techniques for example. Patients with large soft tissue layers are usually associated with obesity and Body Mass Index (BMI) larger than 30 kg.m-2.
(iv)Waveguide regularity: The inverse problem approach supposes a regular waveguide with multiple propagation paths. For osteoporotic patients, the internal cortical interface can be irregular and, therefore, implies poor guided wave spectrum images, particularly in the lower part. These patients are usually associated with high solution ambiguity. If the soft tissue or poor positioning can not be considered as the origin of poor spectrum image, and if the lowk parameter value is low, the waveguide is supposedly irregular, and the lowest thickness solution is considered. Approaches based on machine learning, which does not need physical modeling, can also be used29.
As discussed in the introduction, the current gold standard for the detection of patients at risk of fragility fracture is DXA, which faces some limitations: its large size, poor availability in some regions, relatively high cost, and relatively moderate effectiveness. The first limitations could be mitigated with ultrasonic devices, known for their attractive portability and costs. However, the ability to effectively detect patients at risk should be at least equivalent to DXA. In reality, it is sometimes expected to be higher than DXA to justify the adaptation of most of the references (medical decisions, treatment, costs, rooms, etc.) linked to the gold standard. That is why some ultrasonic devices propose aBMD surrogates10,11,42. However, one drawback of clinical parameters, such as aBMD and also vFAS, is the integration of different cortical bone properties. That is why complementary points of view are proposed by other ultrasonic devices, including BDAT, proposing parameters that can be more easily interpreted by the physician and the patient, such as cortical porosity, thickness, or pore-size distribution9. These parameters reflect geometrical and material properties: cortical bone can be potentially assessed in terms of independent variations of quantity or quality. This point of view could be very helpful in exploring different possible causes of bone fragility. For instance, intra- or extra-capsular hip fragility fractures, i.e., femoral neck or trochanter fracture sites, are supposed to have different medical origins43. Likewise, it could be possible to follow different medications aimed at different effects on cortical bone, as well as in terms of quantity or quality3.
Note that the precision obtained with BDAT for cortical thickness (0.1 mm) is better than other ultrasonic methods, usually above 0.25 mm44. This difference is partly due to the fact that the BDAT inverse problem takes into account combined geometrical and material variations. Some other approaches, such as in pulse echo consider unique bone material properties for all patients10,44. This precision value, about 0.1 mm (CV about 3%), is indeed crucial to finely discriminate between patients as the thickness range is less than 2 mm. The precision on cortical porosity (1%, CV about 14%) is not yet as good as for thickness. However, significant improvements have already been observed with respect to the previous reproducibility study22. One can expect that similar improvements could be achieved in the near future thanks to future HMI improvements, particularly in terms of frame rate closer to real-time.
The BDAT could be used on a large scale for population screening in regions where DXA is not widely available. Moreover, the latest clinical results showed the potential that the BDAT could be even more efficient than DXA. However, these results should be confirmed by including more patients. The next challenge should be multicentre and/or longitudinal studies11,12. However, the BDAT device is still a prototype disponible for scientific collaboration as it has already been done in Germany23 and UK24. Efforts are needed towards the industrialization of the next generation of BDAT devices, which would be&#160;surely faster and more portable.

Osteoporosis and associated fragility fractures still constitute a major public health problem1. In particular, the worldwide number of hip fractures is expected to double until 20502. Bone fragility is due to a slow and silent process of demineralization and bone loss without major alert signs before the fragility fracture event. The current gold standard to detect patients at risk of fragility fracture is the dual X-ray absorptiometry (DXA), providing a 2D, low-resolution X-ray image with a calibrated gray pixel3. From this image, it is possible to extract the areal bone mineral density (aBMD in g.cm-2) at different regions of interest associated with the main fragility fracture sites: spine, wrist, and hip. The aBMD value decreases as the fragility fracture rate increases3. Moreover, the T-score normalization, with respect to a normal healthy population, allows the comparison of patients measured with devices proposed by different manufacturers. The DXA T-score has been proposed by the World Health Organization to define the osteoporosis diagnostic in three stages: normal (T-score &#60; -1), osteopenic (- 1 &#60; T-score &#60; -2.5), and osteoporotic (T-score &#60; -2.5)4.
DXA presents several limitations: its size, relatively high cost, need for a dedicated room, and its ability to discriminate between fractured and non-fractured, as well as its availability in numerous countries, such as in Latin America, are both moderate5. Thus, there is a need for DXA alternatives as screening tools for fragility fracture risk estimation6. However, some DXA alternatives, such as quantitative computerized tomography and its derivatives7, magnetic resonance imaging (MRI)8, are also bulky and not widely available. Quantitative ultrasound (QUS) presents the potential for portable, robust, easy-to-use screening devices. Different devices have been developed for cortical bone assessment, associated with different frequencies ranging from a few kHz to a few MHz and different transducer positioning in transmission, retro diffusion9, pulse echo10, and axial transmission where the transducers are aligned with the axis of a long bone such as radius and tibia. Some devices provide aBMD surrogates11, whereas others provide &#34;classical&#34; ultrasonic parameters such as velocities12 or attenuation coefficients9 and even geometrical and material parameters, for instance, cortical thickness, porosity, or pore size distribution9. However, to this day, QUS has not yet succeeded in being widely used in clinical practice for bone assessment, partly due to the lack of homogenization between devices and operator dependency13.
Amongst QUS technologies proposed as DXA alternatives, axial transmission (AT) has the advantage that the measurement can be performed at the forearm, a site (i) easily accessible and (ii) close to one of the major sites of fragility fractures, i.e., the wrist. The first proposed AT parameter depends on the ultrasonic propagation velocity in the cortical layer, denoted speed of sound (SOS) or velocity of the first arrival signal (vFAS), depending on the signal processing and the devices, some being commercial12,14 ones and other laboratory prototypes15,16. This parameter has been able to discriminate between patient groups with or without fragility fractures with performances similar to BMD in several clinical studies since the late 1990s14,15. It has also been successfully applied for multicentre longitudinal studies, demonstrating its clinical application and robustness12. The vFAS precision has been improved by combining the two opposite directions of propagation in order to reduce the bias due to the angle between the probe and the bone surface16,17. This point of view has been denoted bi-directional AT (BDAT).
Even if vFAS has shown clinical interest, its main drawback, similar to BMD, is that it combines different key cortical bone features such as geometrical and material properties, making its clinical interpretation not straightforward. That is why the guided wave point of view has been proposed, considering its potential due to the fine sensitivity of guided waves to the waveguide properties. This approach should combine signal processing, waveguide modeling, and inverse problems and is largely used in non-destructive testing considering, for instance, metallic waveguides, such as plates or tubes18. Thus, a second-generation BDAT device has been developed step by step since 2010, from bone-mimicking phantoms19 to ex vivo validation20 and in vivo measurements21. The device has been successfully tested in clinical studies in France22, Germany23, United Kingdom24, and Chile25, and it has shown improving results in terms of success rate and patient discrimination.
This study aims to explore the reproducibility of the current BDAT ultrasonic device. First, the device and the measurement protocol will be detailed. Results obtained with 14 participants and 3 operators will be presented and discussed in terms of population screening for the detection of patients at risk of fragility fractures.
Measurement principle: signal processing, parameters of interest, and quality parameters 
The bi-directional axial transmission (BDAT) device is composed of different parts, the main being the ultrasonic probe, the electronic module, and the computer. The complete list is detailed in the Table of Materials and illustrated in Figure 1. In the following, the parameters of interest, the measurement quality parameters, and the measurement protocol are described.
vFAS 
Once the sampled signals are received by the computer, they are processed following different steps. The first step consists of signal processing in the time domain, detecting the FAS using the protocol described previously16,17. Once the arrival time is obtained for each receiver, it is possible to determine the FAS velocity, later denoted vFAS, which is the harmonic mean of the velocities obtained in both directions of propagation. Combining the information from both directions of propagation, it is possible to obtain the value angle between the probe and bone surface directions and to derive an unbiased vFAS value16. This bi-directional angle is later denoted alpha and is used as a parameter of measurement quality. This temporal processing also allows the estimation of the thickness of soft tissue between the bone surface and the probe, denoted ST.Th26.
Guided wave spectrum image 
The second step consists of signal processing in the Fourier domain, considering the temporal and spatial frequencies, denoted f and k. The approach is an SVD-based method, allowing the transformation of the spatio-temporal signals into the Norm function, also denoted guided wave spectrum image (GWSI), as illustrated in Figure 2 for an in vivo forearm19. The method combines two Fourier transforms (time and space) and a singular value decomposition (SVD), allowing one to visualize the presence rate in the received signals (in a 0-1 scale) of the modes guided by the cortical bone layer. The GWSI can be interpreted as an enhancement of the spatio-temporal Fourier Transform, with each pixel being associated with an independent plane of frequency f and wavenumber k. Note that the approach has been improved in order to take into account the impact of material attenuation27 and linear thickness variation28.
Particular attention will be given to the upper part of the spectrum, associated with the A0 mode, and also to the lowest part, associated with the highest phase velocity values, i.e., larger than 4 mm&#183;&#181;s-1. This part corresponds to the region of interest 3 (ROI 3)29. The mean value of ROI 3, later denoted lowk, is also used as a quality parameter. A large value corresponds to a regular waveguide, allowing clear wave reflections at the bone interfaces. If the value decreases, it could be due to an irregular waveguide or a misplaced probe.
Waveguide model 
The guided wave dispersion, or the variation of the phase velocity of each guided mode with respect to the frequency, depends on both the material and geometric properties of the waveguide. Thus, it is potentially possible to retrieve these properties using dedicated signal processing, waveguide modeling, and inverse problem schemes. In the BDAT case, the waveguide model corresponds to a 2D-transverse isotropic free plate, depending on the waveguide material and one geometric parameter, the thickness30. The cortical bone material is homogenized considering fixed parameters for the bone matrix and variable porosity31. Thus, the inverse problem depends on two parameters, denoted cortical thickness (Ct.Th) and cortical porosity (Ct.Po). The effects of material absorption, waveguide curvature, and surrounding soft tissues are not taken into account in the model, even if they impact the measurement. However, their weight on the inverse problem result was not found to be determinant, meaning that the modes in the two main regions of interest (A0 and lowest part) are not significantly changed by the curvature and the soft tissues32.
Inverse problem 
Initially, the inverse problem was divided into two steps: first, extract the experimental guided wave dispersion, and second, compare with the waveguide model. This point of view was limited by noise and mode labeling30,32. Thus, a dedicated approach was proposed to overcome these limitations as an extension of the Norm function point of view. Instead of considering each plane wave independently, only the possible guided waves provided by the waveguide model are taken into account20. This leads to the inverse problem image, expressed in the model parameter domain, i.e., the Ct.th - Ct.Po plane (Figure 2 bottom right). The best-fitting model is given the maximum position, while eventual secondary peaks (indicated by the inverse problem images with a gray dot) correspond to ambiguous solutions, indicated in the f-k comparison with experimental modes with light gray lines. As before, the pixel value is normalized by construction and reflects, in this case, the presence of one particular waveguide model in the received signals. The maximum value (denoted max) and the difference with the second maximum (denoted diff) are also used as quality parameters.
The inverse problem has been originally proposed for offline calculation, i.e., once the signals are acquired, using the exact values of the model wave numbers. This approach has been validated for both radius and tibia sites considering ex vivo20,33 and in vivo21,34,35 studies. In order to include these calculations in the human-machine interface (HMI), an approximated version, compatible with the real-time application, has been proposed, using a sparse matrix point of view36.
vA0 
From the GWSI, it is also possible to extract the velocity of the slowest guided mode, associated with the first antisymmetric mode A0 of the free plate or Lamb model33,35. The upper part of the guided wave spectrum can be linearly approximated, with the slope providing the value of the velocity vA0 (Figure 2 bottom left).
Parameter summary: 
Finally, four parameters of interest are measured: (i) vFAS: velocity of the First Arriving Signal (m&#183;s-1); (ii) vA0: velocity of the slowest guided mode (m&#183;s-1); (iii) Ct.Th: cortical thickness (mm); and (iv) Ct.Po: cortical porosity (%).
Four quality parameters are considered: (i) alpha: bi-directional angle (&#176;); (ii) lowk: mean value of lowest part of the GWSI (normalized value between 0 and 1); (iii) max: maximum of the inverse problem function (normalized value between 0 and 1); and (iv) diff: the difference between the first and second maxima of the inverse problem function (normalized value between 0 and 100).
All these parameters, as well as the two guided wave spectrum images (one par direction of propagation) and the inverse problem image, are displayed in &#34;real-time&#34; by the HMI, with a frame rate of about 2 Hz. A typical example is illustrated in Figure 3. In the following section, the method of using these parameters is described in detail. The main idea is that the operator moves the probe slowly at the measurement site, carefully observing the feedback provided by the different parts of the interface until finding a stable position and starting a series of 10 acquisitions. When at least four consistent series are obtained, the measurement ends, and an automatic report is generated.

<table><tbody><tr><td>Computer</td><td>Notebook HP</td><td>mod Zbook (16 Go RAM, Inrel Core i7)</td><td>to receive the sampled signals and applying the signal processing steps. Results are displayed in quasi real time (up to 4 per second) through a dedicated Human Machine Interface (HMI, BleuSolid, Pomponne, France) allowing the measurement guidance;</td></tr><tr><td>Electric insulation transformer&amp;nbsp;</td><td>REOMED, Solingen, Germany</td><td>IEC / EN 60601-1</td><td>to protect the device, the patient and other devices from any electric hazard</td></tr><tr><td>Electronic module</td><td>Altha&amp;iuml;s, Tours, France</td><td>in-house</td><td>send excitation signals (half a period of negative voltage of 170 V) and discretize received signals (1024 time points per receiver at 20 MHz sampling frequency and 12 bit dynamic) before sending them to the computer. Delays and amplification can be adapted using linear laws in order to optimize data sampling within the accessible ranges.</td></tr><tr><td>Human Machine Interface</td><td>BleuSolid, Pomponne, France</td><td>N/A</td><td>HMI</td></tr><tr><td>Pedal switch</td><td>Scythe, Germany</td><td>USB Foot Switch 2</td><td>to start an acquisition series</td></tr><tr><td>Ruler</td><td>Westcott, USA</td><td>10417</td><td>to locate the measurement site</td></tr><tr><td>Ultrasonic probe radius</td><td>Vermon, Tours, France</td><td>in-house</td><td>1 MHz central frequency, 24 receivers with 0.8 mm pitch and two blocks of 5 transmitters with 1 mm pitch.&amp;nbsp;</td></tr><tr><td>Ultrasonic probe tibia</td><td>Vermon, Tours, France</td><td>in-house</td><td>0.5 MHz central frequency, 24 receivers with 1.2 mm pitch and two block of 5 transmitters with 1.5 mm pitch</td></tr><tr><td>Ultrasonic probes</td><td></td><td></td><td>designed according to the bidirectional geometry: a single receiver array surrounded by two transmitter arrays. The three arrays are aligned, mechanically and electrically isolated in order to minimize coupling signals. &amp;nbsp; The probes are adapted to two different sites, one third distal radius and mid tibia.</td></tr></tbody></table>

cortical bone assessment using ultrasonic guided waves: a reproducibility study in a healthy population

Fragility fractures are still a worldwide health burden in the context of population aging. In particular, the global number of hip fractures is expected to double between 2020 and 2050. Therefore, it is essential to detect patients at risk of fragility fracture at the population scale. The current golden standard is dual X-ray absorptiometry (DXA), providing the areal bone mineral density (aBMD). Ultrasonic devices, usually more portable and cheaper than X-ray devices, represent interesting DXA alternatives as screening tools. However, operator dependency is usually recognized as their main drawback. In this study, the measurement protocol of the bi-directional axial transmission (BDAT) ultrasonic device is presented in detail. The dedicated ultrasonic probe is placed at the one-third distal radius of the non-dominant forearm using conventional coupling gel. The guided interface provides in quasi-real time (about 2 Hz) four parameters of interest: velocities of the first arriving signal (vFAS) and the A0 mode (vA0),&#160;cortical thickness (Ct.Th) and porosity (Ct.Po), as well as four quality parameters. The operator moves the probe slowly at the measurement site, carefully observing the feedback provided by the interface until finding a stable position and starting a series of 10 acquisitions. When at least four consistent series are obtained, the measurement ends, and an automatic report is generated. The measurement usually takes about 5 min to complete. To determine the robustness of this protocol, a reproducibility study was conducted among 3 operators (one expert and two novices) and 14 healthy participants (6 women, 8 men, 21-53 years old). The intraclass correlation coefficients (ICC) were found good for vA0 (0.76), Ct.Po (0.80) or excellent for Ct.Th (0.87) and vFAS (0.91). The standard deviations were found to be less than 10% of the total ranges in clinical practice.

A reproducibility study was conducted considering 3 operators (one expert, two novices) and 14 healthy participants (6 women, 8 men, 21-53 years old). Novice operators were trained for approximately 3 h to understand and practice the acquisition protocol. Then, the participants were measured during 2 weeks in August 2023. Each measurement was performed independently. All operators were blinded, i.e., one operator did not know the results obtained by the two others.
Intra-operator repeatability 
Figure 5&#160;illustrates the intra-operator repeatability for a consistent case: 4 series of 10 acquisitions acquired on one participant and one operator. The first column corresponds to the inverse problem functions, while the second column shows the experimental guided mode dispersion compared with the best-fitting model. Each figure line corresponds to a successful series. The number of retained acquisitions is given in the title: 7 out of 10 for the first series and 10 out of 10 for the following ones. Means and standard deviations and the four parameters (vFAS, vA0, Ct.Th, and Ct.Po) are provided for each series. In addition, quality parameters are also shown: bi-directional angle (alpha), maximum of the inverse problem function (max), the absolute difference with the second maximum (diff), and the mean value of the lowest part of the GWSI (lowk).
Intra-series standard deviations are low, about 0.02 mm for the cortical thickness, less than 0.5% for cortical porosity, and less than 20 m&#8729;s-1 for the two velocities, indicating that the reach probe positions were stable. Then, one can observe that the mean values obtained for each series are very close, particularly for the thickness values ranging between 3.4 mm and 3.5 mm and the vFAS values ranging from 4040 m&#8729;s-1 to 4070 m&#8729;s-1. Note that a difference of 40 m&#8729;s-1 corresponds to a 1% difference for a mean value of 4000 m&#8729;s-1. Larger variations are observed for cortical porosity, ranging from 8% to 12%, and the vA0 velocity, ranging from 1700 m&#8729;s-1 to 1740 m&#8729;s-1. In this consistent case, almost all acquisitions are consistent, i.e., close to each other. There is almost no ambiguity in the final result of the four parameters of interest.
A second case is illustrated in Figure 6 for a less regular case. In this case, three parameters, vFAS, vA0, and Ct.Po are very stable, with values respectively about 4120 m&#8729;s-1, 1700 m&#8729;s-1, and 10%. The case of the cortical thickness is more difficult as two ambiguous solutions, 2.6 mm, and 3.5 mm, are observed in agreement with a small difference value (diff less than 0.5%) between the two first maxima of the inverse problem function. In the previous regular case, this difference was ranging from 1% to 3 %. The ambiguity is removed by expert analysis, in this case, looking at the agreement between experimental and theoretical guided modes (right column). In the case of the lowest thickness, the agreement is better in the lowest part of the spectrum (two first series). For the two last series, there is a theoretical mode with very few experimental points, around 0.5 MHz, indicating a poorer agreement in comparison with the previous series. Moreover, the diff parameter (0.1%) is less than the values of the two first series (0.4% and 0.2%). In this case, the choice for the kept solution (2.6 mm) is not yet automated, and an expert is still needed. However, the three operators faced similar problems and chose similar solutions, close to 2.6 mm.
Extra-operator reliability 
All the results for the 4 parameters of interest obtained by the 3 operators with the 14 participants are shown in Figure 7. The intraclass correlation coefficients (ICC) were computed following the formula and the Matlab code previously published37,38,39. ICC is commonly used for the assessment of the reliability of measurement scales, in particular for biomedical applications. ICC values ranging from 0.75 to 0.9 are usually associated with good reliability, while ICC values above 0.9 are considered excellent reliability. The lowest value for ICC (0.76) was obtained for the parameter vA0. The standard deviation was equal to 17 m&#8729;s-1, which is about 7% of the measurement range of the order of 250 m&#8729;s-1. Similar values were observed for Ct.Po with ICC equal to 0.80, and a standard deviation of 1.1%, about 10% of the range. Excellent reliability (ICC about 0.9) was obtained for the two other parameters, Ct.Th and Ct.Po, with a standard deviation inferior to 10% of the range.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/66985/66985fig01.jpg" /> 
Figure 1: different parts of the Bi-Directional Axial Transmission (BDAT) ultrasonic device. The prototype includes electronic insulation (1), a pedal switch (2), two probes (3.1 and 3.2), an electronic module (4), a computer (5), and a ruler (6). <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 2" class="xfigimg" src="/files/ftp_upload/66985/66985fig02.jpg" /> 
Figure 2: Typical acquisition on in vivo forearm. The two Norm functions (one per direction of propagation) are shown in the upper line of the image. They are also denoted guided wave spectrum image (GWSI). From the maxima of these images, it is possible to extract the experimental guided modes (blue and red dots) compared with the best-fitting model (bottom left sub-image). The best-fitting model is parametrized by two values, cortical thickness (Ct.Th) and porosity (Ct.Po), corresponding to the maximum position of the inverse problem function (bottom right sub-image). Their values are shown in the title of each panel. The vA0 fitting is shown as a dashed line (left). The Guided Wave Spectrum Images and the inverse problem image are normalized (i.e., the pixel value ranges from 0 to 1) by construction19,20. The values of the two measured velocities, vFAS and vA0, are indicated in the title of the top right sub-image. <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig02large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 3" class="xfigimg" src="/files/ftp_upload/66985/66985fig03.jpg" /> 
Figure 3: Human machine interface (HMI). The HMI shows in real the two GWSIs (one per propagation direction), the inverse problem image, the parameters of interest, and the quality parameters. <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig03large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 4" class="xfigimg" src="/files/ftp_upload/66985/66985fig04.jpg" /> 
Figure 4: Example of automated report. The report indicated the participant&#39;s and operator&#39;s data as well as the final values of the parameters of interest and quality parameters. <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig04large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 5" class="xfigimg" src="/files/ftp_upload/66985/66985fig05.jpg" /> 
Figure 5: Example of the second report for a consistent case, same as the automated report shown in Figure 4. The figure shows 4 series of 1 participant and 1 operator: inverse problem images (left column) and experimental wavenumbers compared to the best fitting model (right column). The shown values correspond to the mean and standard deviation of the kept acquisitions over the 10-acquisition series. The number of kept acquisitions is indicated in the title of the right column, for example, (7/10) for the first series. <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig05large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 6" class="xfigimg" src="/files/ftp_upload/66985/66985fig06.jpg" /> 
Figure 6: Example of the second report for an ambiguous case for cortical thickness. The figure shows 4 series of 1 participant and 1 operator: inverse problem images (left column) and experimental wavenumbers compared to the best fitting model (right column). The shown values correspond to the mean and standard deviation over the kept acquisitions over the 10-acquisition series. The number of kept acquisitions is indicated in the title of the right column, for example, (10/10) for the first series. <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig06large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
<img alt="Figure 7" class="xfigimg" src="/files/ftp_upload/66985/66985fig07.jpg" /> 
Figure 7: Inter-operator reliability. Results are shown for the 4 parameters of interest obtained by the 3 operators with 14 participants. The values obtained by the 3 operators (y-axis) are compared with the mean value of the 3 operators (x-axis). The intraclass correlation coefficients (ICC) are indicated in the titles. The standard deviation &#963; and the coefficient of variation CV are also indicated. <a href="https://jove.unicartagenaproxy.elogim.com/files/ftp_upload/66985/66985fig07large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>

Cortical Bone Assessment Using Ultrasonic Guided Waves: A Reproducibility Study in a Healthy Population

Here, we present the measurement protocol of the bi-directional axial transmission (BDAT) ultrasonic device in detail and test it in a reproducibility study, considering 14 healthy participants and 3 operators. Reliability, measured with the intraclass correlation coefficients (ICC), was good to excellent for four parameters of interest.

Fragility fractures are still a worldwide health burden in the context of population aging. In particular, the global number of hip fractures is expected ...

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Universidad de Cartagena UDEC

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JoVE Journal

Bioengineering

339 Views.  Ecole Centrale. Here, we present the measurement protocol of the bi-directional axial transmission (BDAT) ultrasonic device in detail and test it in a reproducibility study, considering 14 healthy participants and 3 operators. Reliability, measured with the intraclass correlation coefficients (ICC), was good to excellent for four parameters of interest. 

Video: Cortical Bone Assessment Using Ultrasonic Guided Waves: A Reproducibility Study in a Healthy Population

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Microcontact printing provides a rapid, highly reproducible method for the creation of well-defined patterned substrates.1 While microcontact printing can be employed to directly print a large number of molecules, including proteins,2 DNA,3 and silanes,4 the formation of self-assembled monolayers (SAMs) from long chain alkane thiols on gold provides a simple way to confine proteins and cells to specific patterns containing adhesive and resistant regions. This confinement can be used to control cell morphology and is useful for examining a variety of questions in protein and cell biology. Here, we describe a general method for the creation of well-defined protein patterns for cellular studies.5 This process involves three steps: the production of a patterned master using photolithography, the creation of a PDMS stamp, and microcontact printing of a gold-coated substrate. Once patterned, these cell culture substrates are capable of confining proteins and/or cells (primary cells or cell lines) to the pattern.
The use of self-assembled monolayer chemistry allows for precise control over the patterned protein/cell adhesive regions and non-adhesive regions; this cannot be achieved using direct protein stamping. Hexadecanethiol, the long chain alkane thiol used in the microcontact printing step, produces a hydrophobic surface that readily adsorbs protein from solution. The glycol-terminated thiol, used for backfilling the non-printed regions of the substrate, creates a monolayer that is resistant to protein adsorption and therefore cell growth.6 These thiol monomers produce highly structured monolayers that precisely define regions of the substrate that can support protein adsorption and cell growth. As a result, these substrates are useful for a wide variety of applications from the study of intercellular behavior7 to the creation of microelectronics.8
While other types of monolayer chemistry have been used for cell culture studies, including work from our group using trichlorosilanes to create patterns directly on glass substrates,9 patterned monolayers formed from alkane thiols on gold are straight-forward to prepare. Moreover, the monomers used for monolayer preparation are commercially available, stable, and do not require storage or handling under inert atmosphere. Patterned substrates prepared from alkane thiols can also be recycled and reused several times, maintaining cell confinement.10

Self-assembled monolayers (SAMs) formed from long chain alkane thiols on gold provide well-defined substrates for the formation of protein patterns and cell confinement. Microcontact printing of hexadecanethiol using a polydimethylsiloxane (PDMS) stamp followed by backfilling with a glycol-terminated alkane thiol monomer produces a pattern where protein and cells adsorb only to the stamped hexadecanethiol region.

Microcontact printing provides a rapid, highly reproducible method for the creation of well-defined patterned substrates.1  While microcontact printing ...

Non-invasive Parenchymal, Vascular and Metabolic High-frequency Ultrasound and Photoacoustic Rat Deep Brain Imaging

Photoacoustics and high frequency ultrasound stands out as powerful tools for neurobiological applications enabling high-resolution imaging on the central nervous system of small animals. However, transdermal and transcranial neuroimaging is frequently affected by low sensitivity, image aberrations and loss of space resolution, requiring scalp or even skull removal before imaging. To overcome this challenge, a new protocol is presented to gain significant insights in brain hemodynamics by photoacoustic and high-frequency ultrasounds imaging with the animal skin and skull intact. The procedure relies on the passage of ultrasound (US) waves and laser directly through the fissures that are naturally present on the animal cranium. By juxtaposing the imaging transducer device exactly in correspondence to these selected areas where the skull has a reduced thickness or is totally absent, one can acquire high quality deep images and explore internal brain regions that are usually difficult to anatomically or functionally describe without an invasive approach. By applying this experimental procedure, significant data can be collected in both sonic and optoacoustic modalities, enabling to image the parenchymal and the vascular anatomy far below the head surface. Deep brain features such as parenchymal convolutions and fissures separating the lobes were clearly visible. Moreover, the configuration of large and small blood vessels was imaged at several millimeters of depth, and precise information were collected about blood fluxes, vascular stream velocities and the hemoglobin chemical state. This repertoire of data could be crucial in several research contests, ranging from brain vascular disease studies to experimental techniques involving the systemic administration of exogenous chemicals or other objects endowed with imaging contrast enhancement properties. In conclusion, thanks to the presented protocol, the US and PA techniques become an attractive noninvasive performance-competitive means for cortical and internal brain imaging, retaining a significant potential in many neurologic fields.

The present work describes a new protocol to perform non-invasive high-frequency ultrasound and photoacoustic based imaging on rat brain, to efficiently visualize deep subcortical regions and their vascular patterns by directing signals on skull foramina naturally present on animal cranium.

Photoacoustics and high frequency ultrasound stands out as powerful tools for neurobiological applications enabling high-resolution imaging on the central ...

Neuroscience

Proximal Cadaveric Femur Preparation for Fracture Strength Testing and Quantitative CT-based Finite Element Analysis

Cadaveric fracture testing is routinely used to understand factors that affect proximal femur strength. Because ex vivo biological tissues are prone to lose their mechanical properties over time, specimen preparation for experimental testing must be performed carefully to obtain reliable results that represent in vivo conditions. For that reason, we designed a protocol and a set of fixtures to prepare the femoral specimens such that their mechanical properties experienced minimal changes. The femora were kept in a frozen state except during preparation steps and mechanical testing. The relevant clinical measures of total hip and femoral neck bone mineral density (BMD) were obtained with a clinical dual X-ray absorptiometry (DXA) bone densitometer, and the 3D geometry and distribution of bone mineral were obtained using CT with a calibration phantom for quantitative estimations based on the greyscale values. Any possible bone disease, fracture, or the presence of implants or artifacts affecting the bone structure, was ruled out with X-ray scans. For preparation, all bones were carefully cleaned of excess soft tissue, and were cut and potted at the internal rotation angle of interest. A cutting fixture allowed the distal end of the bone to be cut off leaving the proximal femur at a desired length. To allow positioning of the femoral neck at prescribed angles during later CT scanning and mechanical testing, the proximal femoral shafts were potted in polymethylmethacrylate (PMMA) using a fixture designed specifically for desired orientations. The data collected from our experiments were then used for validation of quantitative computed tomography (QCT)-based finite element analysis (FEA), as described in a different protocol. In this manuscript, we present the protocol for the precise bone preparation for mechanical testing and subsequent QCT/FEA modeling. The current protocol was successfully applied to prepare about 200 cadaveric femora over a 6-year time period.

We present a robust protocol on how to carefully preserve and prepare cadaveric femora for fracture testing and quantitative computed tomography imaging. The method provides precise control over input conditions for the purpose of determining relationships between bone mineral density, fracture strength, and defining finite element model geometry and properties.

Cadaveric fracture testing is routinely used to understand factors that affect proximal femur strength.  Because ex vivo biological tissues are prone to ...

Medicine

Scanning Skeletal Remains for Bone Mineral Density in Forensic Contexts

The purpose of this paper is to introduce a promising, novel method to aid in the assessment of bone quality in forensically relevant skeletal remains. BMD is an important component of bone&#39;s nutritional status and in skeletal remains of both juveniles and adults, and it can provide information about bone quality. For adults remains, it can provide information on pathological conditions or when bone insufficiency may have occurred. In juveniles, it provides a useful metric to elucidate cases of fatal starvation or neglect, which are generally difficult to identify. This paper provides a protocol for the anatomical orientation and analysis of skeletal remains for scanning via dual-energy X-ray absorptiometry (DXA). Three case studies are presented to illustrate when DXA scans can be informative to the forensic practitioner. The first case study presents an individual with observed longitudinal fractures in the weight bearing bones and DXA is used to assess bone insufficiency. BMD is found to be normal suggesting another etiology for the fracture pattern present. The second case study employed DXA to investigate suspected chronic malnutrition. The BMD results are consistent with results from long bone lengths and suggest the juvenile had suffered from chronic malnutrition. The final case study provides an example where fatal starvation in a fourteen-month infant is suspected, which supports autopsy findings of fatal starvation. DXA scans showed low bone mineral density for chronological age and is substantiated by traditional assessments of infant health. However, when dealing with skeletal remains taphonomic alterations should be considered before applying this method.

Bone mineral density (BMD) is an important factor in understanding nutritional intake. For human skeletal remains, it is a useful metric to assess quality of life in both juveniles and adults, particularly in fatal starvation and neglect cases. This paper provides guidelines for scanning human skeletal remains for forensic purposes.

The purpose of this paper is to introduce a promising, novel method to aid in the assessment of bone quality in forensically relevant skeletal remains. ...

Ultrasound Evaluation of Achilles Tendon Morphology

Measurement of Healthy and Injured Triceps Surae Morphology

Achilles tendon injuries occur throughout the lifespan and can negatively affect quality of life and overall health. Achilles tendinopathy is generally classified as an overuse injury associated with fusiform tendon thickening, neovascularization, and interstitial tendon degeneration. Current literature suggests these structural changes are associated with&#160;symptoms and lower physical activity levels, as well as&#160;symptoms and lower extremity function in the long term. Surgically and non-surgically managed Achilles tendon ruptures result in increased tendon cross-sectional area (CSA) and a lengthened Achilles tendon. Both structural outcomes have clinical implications, as larger CSA positively predicts function, whereas increased tendon lengthening predicts reduced function after Achilles tendon rupture. Given the relationship between structural changes associated with Achilles tendon injuries for both injury severity and injury recovery, it is critical to be able to quantify Achilles tendon structure reliably and accurately. Silbernagel&#39;s group has established a valid and reliable method for efficiently evaluating triceps surae muscle and tendon structure. In this protocol, B-mode musculoskeletal ultrasound imaging is used to measure triceps surae structure, including Achilles tendon thickness and CSA, soleus thickness, and the presence of additional findings (calcifications and bursitis). B-mode extended field-of-view is used to measure Achilles tendon length and gastrocnemius anatomical CSA. Finally, power Doppler is used to identify intratendinous neovascularization. Quantification of triceps surae structure allows for comparison between limbs as well as longitudinal changes in response to exercise and treatment for healthy individuals and those with Achilles tendon injuries. This protocol has been used in many research studies to date and proves valuable in understanding the relationship between tendon structure and injury development, severity, and recovery. As ultrasound devices are becoming more affordable and portable, this protocol proves promising as a clinical tool, given its quick and efficient methods.

Author Spotlight: Exploring the Complexities of Achilles Tendon Injuries &#8212; Research and Future Directions

Ultrasound imaging is becoming more accessible in clinical and research settings, and a consistent protocol will be beneficial for comparison between studies and for clinical interpretations. This protocol for ultrasound evaluation is a valid and reliable method to evaluate Achilles tendon morphology in healthy, tendinopathic, and ruptured tendons.

Achilles tendon injuries occur throughout the lifespan and can negatively affect quality of life and overall health. Achilles tendinopathy is generally ...

A Sectioning, Coring, and Image Processing Guide for High-Throughput Cortical Bone Sample Procurement and Analysis for Synchrotron Micro-CT

Bone is a dynamic and mechanically active tissue that changes in structure over the human lifespan. The products of the bone remodeling process have been studied substantially using traditional two-dimensional techniques. Recent advancements in X-ray imaging technology via desktop micro-computed tomography (&#181;CT) and synchrotron radiation micro-computed tomography (SR&#181;CT) have allowed for the acquisition of high-resolution three-dimensional (3D) scans of a larger field of view (FOV) than other 3D imaging techniques (e.g., SEM) providing a more complete picture of microscopic structures within human cortical bone. The specimen should be accurately centered within the FOV, however, to limit the appearance of streak artifacts known to impact data analysis. Previous studies have reported procurement of irregularly shaped rectilinear bone blocks that result in imaging artifacts due to uneven edges or image truncation. We have applied a geological sampling protocol (coring) to procure consistently sized cortical bone core specimens for SR&#181;CT experiments from the anterior aspect of human femora. This coring method is efficient and minimally destructive to tissue. It creates uniform cylindrical samples that decrease imaging artifacts by nature of being isometric during rotation and providing a uniform path length for X-ray beams throughout scanning. Image processing of X-ray tomographic data of cored and irregularly shaped samples confirms the potential of the technique to improve visualization and analysis of cortical bone microarchitecture. A goal of this protocol is to deliver a reliable and repeatable method for the extraction of cortical bone cores that is adaptable for various types of high-resolution bone imaging experiments. An overarching goal of the work is to create a standardized cortical bone procurement for SR&#181;CT that is affordable, consistent, and straightforward. This procedure may further be adapted by researchers in related fields who commonly evaluate hard composite materials such as in biological anthropology, geosciences, or material sciences.

We employed a geological (coring) sampling protocol to procure cortical bone specimens of uniform size for SR&#181;CT experiments from the anterior aspect of human femora. This method is minimally destructive, efficient, results in cylindrical specimens that minimize imaging artifacts from irregular sample shapes and improves microarchitectural visualization and analysis.

Bone is a dynamic and mechanically active tissue that changes in structure over the human lifespan. The products of the bone remodeling process have been ...

Biology

Ultrasound Protocol for Perioperative Frailty Assessment

Identifying Frailty Using Point-of-Care Ultrasonography: Image Acquisition and Assessment

Frailty is a significant predictor of a range of adverse outcomes in surgical patients, including increased mechanical ventilation time, longer hospital stays, unplanned readmissions, stroke, delirium, and death. However, accessible tools for screening in clinical settings are limited. Computed tomography of the psoas muscle is the current standard imaging device for measuring frailty, but it is expensive, time-consuming, and exposes the patient to ionizing radiation. Recently, the use of point-of-care ultrasound (POCUS) has emerged as a potential tool to determine the presence of frailty and has been shown to accurately predict frailty and postoperative outcomes. In this article, we will describe the image acquisition of the quadriceps muscles and explain how they can be used to determine frailty and predict postoperative adverse events. We will present information on probe selection, patient positioning, and troubleshooting. Images from a demonstration will be used to present the POCUS technique and example results. The article will culminate in a discussion of the use of these images in medical decision-making and potential limitations.

Author Spotlight: Assessing Surgical Frailty with Point-of-Care Ultrasound of Quadriceps Muscles

Here, we present a protocol to assess frailty in the perioperative setting using point-of-care ultrasound to measure quadriceps thickness. This method offers a practical, non-invasive alternative to traditional assessment methods, potentially enhancing perioperative care by quickly identifying frail patients.

Frailty is a significant predictor of a range of adverse outcomes in surgical patients, including increased mechanical ventilation time, longer hospital ...

Assessment of Bone Fracture Healing Using Micro-Computed Tomography

Micro-computed tomography (&#181;CT) is the most common imaging modality to characterize the three-dimensional (3D) morphology of bone and newly formed bone during fracture healing in translational science investigations. Studies of long bone fracture healing in rodents typically involve secondary healing and the formation of a mineralized callus. The shape of the callus formed and the density of the newly formed bone may vary substantially between timepoints and treatments. Whereas standard methodologies for quantifying parameters of intact cortical and trabecular bone are widely used and embedded in commercially available software, there is a lack of consensus on procedures for analyzing the healing callus. The purpose of this work is to describe a standardized protocol that quantitates bone volume fraction and callus mineral density in the healing callus. The protocol describes different parameters that should be considered during imaging and analysis, including sample alignment during imaging, the size of the volume of interest, and the number of slices that are contoured to define the callus.

Micro-computed tomography (&#181;CT) is a non-destructive imaging tool that is instrumental in assessing bone structure in preclinical studies, however there is a lack of consensus on &#181;CT procedures for analyzing the bone healing callus. This study provides a step-by-step &#181;CT protocol that allows the monitoring of fracture healing.

Micro-computed tomography (&#181;CT) is the most common imaging modality to characterize the three-dimensional (3D) morphology of bone and newly formed ...

High-frequency Ultrasound Imaging of the Abdominal Aorta

Source: Amelia R. Adelsperger, Evan H. Phillips, and <a href="https://jove.unicartagenaproxy.elogim.com/author/Craig+J._Goergen">Craig J. Goergen</a>, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana
High-frequency ultrasound systems are used to acquire high resolution images. Here, the use of a state-of-the-art system will be demonstrated to image the morphology and hemodynamics of small pulsatile arteries and veins found in mice and rats. Ultrasound is a relatively inexpensive, portable, and versatile method for the noninvasive assessment of vessels in humans as well as large and small animals. These are several key advantages that ultraound offers compared to other techniques, such as computed tomography (CT), magnetic resonance imaging (MRI), and near-infrared fluorescence tomography (NIRF). CT requires ionizing radiation and MRI can be prohibitively expensive and even impractical in some scenarios. NIRF, on the other hand, is limited by the penetration depth of light required to excite the fluorescent contrast agents.
Ultrasound has limitations in terms of imaging depth; however, this may be overcome by sacrificing resolution and using a lower frequency transducer. Abdominal gas and excess body weight can severely diminish image quality. In the first case, the propagation of sound waves is limited, while in the latter case, they are attenuated by overlying tissues, such as fat and connective tissue. As a result, no contrast or faint contrast may be observed. Finally, ultrasound is a highly user-dependent technique, requiring the sonographer to be familiar with anatomy and to be able to work around issues, such as the appearance of imaging artifacts or acoustic interference.

High-frequency Ultrasound Imaging and Image Acquisition

Source: Amelia R. Adelsperger, Evan H. Phillips, and Craig J. Goergen, Weldon School of Biomedical Engineering, Purdue University, West Lafayette, 
...

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JoVE Science Education

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Biomedical Engineering

Assessing Bone Mineral Density Using Radiofrequency Echographic Multi-Spectrometry

This video demonstrates the radiofrequency echographic multi-spectrometry (REMS) technique for assessing bone mineral density (BMD). The patient is positioned supine, and ultrasound gel is applied to the femoral neck, a fracture-prone site in the femur. The probe then emits ultrasound waves, which reflect off the bone tissue at a speed influenced by BMD. The probe captures these reflected waves, enabling the quantification of BMD.

This video demonstrates the radiofrequency echographic multi-spectrometry (REMS) technique for assessing bone mineral density (BMD). The patient is ...