The authors thank Dr. Wen Yi and other medical staff of the Department of Gynecology, Hospital of Chengdu University of Traditional Chinese Medicine for their help.

All procedures of this study were conducted in accordance with clinical trial registration and have been approved by the Ethics Committee of the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine (Filing No. 2021KL-004). All patients in this study provided informed consent to the investigators&#39; use of data, images, and related video filming during the trial.
1. Preparation before operation
<ol>
	<li>Set the inclusion criteria as follows: include patients meeting the diagnostic criteria for pelvic inflammatory disease in Western medicine8,9 and the dampness heat stasis syndrome type in traditional Chinese medicine10, aged 20-25 years old.</li>
	<li>Set the following exclusion criteria: other causes of pelvic pain or lower abdominal pain; pregnancy, lactation, or preparing for pregnancy within the past 6 months; severe conditions affecting the heart, liver, kidneys, and hematopoietic system that make treatment intolerable; inability or unwillingness to cooperate; individuals prone to allergies or keloid formation; history of similar medication or physical treatments within the last month.</li>
	<li>Get the following materials ready: sterile gloves, disposable catgut embedding needles, disposable thread embedding kit (treatment tray, sterile gauze, sterile cotton swab, iodophor cotton swab, tweezers, &#34;0&#34; catgut, medical patches; Figure 1).</li>
	<li>Ask the patient to lie on her back, relax, and fully expose the abdomen and lower limbs.</li>
	<li>Check the skin condition of the target acupoint location and proceed with the procedure if there are no abnormalities. 
	NOTE: Patients with infection, ulceration, or scars on the skin at the acupoint should not undergo the embedding. Patients should not have the procedure right away if they are extremely hungry, fatigued, or under significant mental stress. Avoid performing the procedure on a menstruating patient.</li>
	<li>Have the physician perform routine hand disinfection and wear sterile gloves.</li>
	<li>Check whether the core of the disposable thread embedding needle and the needle tube match smoothly (Figure 2A).</li>
	<li>Holding tweezers in the right hand, place the catgut into the needle tube (Figure 2B). 
	NOTE: The catgut needs to be completely inserted into the needle tube.</li>
</ol>
2. Operating steps
<ol>
	<li>Select the following acupoints (international code): Zhongji (RN3), Guanyuan (RN4), Qihai (RN6), Guilai (ST29), Zigong (EX-CA1), Xueha (SP10), Zusanl (ST36), Yinlingquan (SP9), and Sanyinjiao (SP6; Figure 3).</li>
	<li>Needle insertion
	<ol>
		<li>Tighten the skin of the needle insertion site with the left hand, and pinch the needle tube with the thumb, index, and middle fingers of the right hand.</li>
		<li>Quickly insert the needle downwards at a 90&#176; angle to the skin (Figure 2C,D).</li>
		<li>When the patient experiences getting qi (needle sensation) such as soreness, numbness, and bloating, stop inserting the needle, push the needle core, and withdraw the needle tube, leaving the catgut in the qi-receiving area.</li>
	</ol>
	</li>
	<li>Needle extraction: Press the skin of the acupoint with the left hand and pull out the needle core and barrel with the right hand. 
	NOTE: The catgut thread must be fully intradermal and should not be left subcutaneous or exposed outside the hypodermic.</li>
	<li>Compress the needle hole with a sterilized dry cotton ball for 30 s and apply a medical application to the needle hole for 2 days.</li>
</ol>
3. Precautions after embedding the catgut
<ol>
	<li>Ensure that the embedded site does not come into contact with water within the first 48 h.</li>
	<li>Advise the patient to avoid strenuous physical activities, especially around the area where the thread was embedded, for 7 days following the procedure.</li>
</ol>
4. Adverse reactions and management
<ol>
	<li>Patient discomfort
	<ol>
		<li>Pain
		<ol>
			<li>Advise patients that pain is a common adverse reaction11 and that their pain will mostly disappear after 5-7 days.</li>
			<li>Use fine thread-embedding needles and catgut bodies during the procedure.</li>
			<li>Consider preoperative oral analgesics for patients who are sensitive to pain and have no known contraindications11; If necessary, administer local anesthesia with lidocaine before embedding the catgut12.</li>
		</ol>
		</li>
		<li>Needle-related fainting 
		NOTE: This refers to the sudden occurrence of dizziness, blurred vision, palpitations, nausea, and even syncope during the acupoint catgut-embedding treatment.
		<ol>
			<li>If the patient faints, immediately stop the catgut embedding and observe the patient&#39;s vital signs. For milder cases, ask the patient to lie down for a while and receive warm water or a sugary drink. In cases where there is no alleviation of the symptoms, employ modern first-aid measures.</li>
		</ol>
		</li>
		<li>Bleeding or local hematoma
		<ol>
			<li>If postoperative bleeding occurs at the embedding site, apply gentle pressure with a dry cotton swab at the local site to stop it.</li>
			<li>If a hematoma forms, apply a cold compress within 24 h, followed by warm compresses after 24 h to promote hematoma absorption. 
			NOTE: If the bleeding persists or is substantial, it may indicate vascular damage, necessitating surgical intervention.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Catgut body rejection reaction
	<ol>
		<li>Fever 
		NOTE: Any fever generally disappears within 1 week.
		<ol>
			<li>Instruct the patients to drink plenty of water. Treat continuous fever with physical cooling or oral antipyretic medications.</li>
			<li>When prolonged high fever occurs, ensure prompt cooling and removal of the embedded catgut by a surgical specialist.</li>
		</ol>
		</li>
		<li>Allergic reactions
		<ol>
			<li>Look for allergic symptoms such as local redness, rash, and itching; provide antihistamine drugs (such as chlorpheniramine maleate, cetirizine, loratadine) or corticosteroids (such as dexamethasone) for allergy management, and if necessary, remove the embedded catgut from the body.</li>
		</ol>
		</li>
		<li>Nodules or masses
		<ol>
			<li>Small nodules usually resolve on their own. For localized firm masses, employ methods like moxibustion and acupoint injections13.</li>
		</ol>
		</li>
		<li>Thread overflow
		<ol>
			<li>Manage thread overflow by trimming or extracting the extruded catgut and then re-embedding it.</li>
		</ol>
		</li>
		<li>Infection
		<ol>
			<li>During the first 5 days after catgut embedding, there may be varying degrees of inflammation at the local site. If there is excessive discharge at the embedding site or signs of purulent changes, treat with antibiotics for infection control. If necessary, seek surgical intervention.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>
5. Observation indicators
<ol>
	<li>Clinical efficacy evaluation14
	<ol>
		<li>Define recovery as the improvement of all related symptoms and signs such as pelvic pain, with a score decrease of &#8805;95%.</li>
		<li>Define a significant effect as 70% &#8804; score reduction &#60; 95%.</li>
		<li>Consider the treatment to be effective if 30% &#8804; score reduction &#60; 70%.</li>
		<li>Consider the treatment to be invalid if the score reduction is &#60;30%.</li>
	</ol>
	</li>
	<li>Use the simplified McGill Pain Scale (SF-MPQ)15 to evaluate pain emotions and severity before and after treatment, with higher scores indicating more severe pain.</li>
	<li>Use the Traditional Chinese Medicine Damp Heat Stasis Syndrome Scale to score Traditional Chinese Medicine Syndrome16. The higher the score, the more severe the symptoms.</li>
	<li>Evaluate abdominal tenderness or rebound pain, uterine tenderness, neck elevation and swing pain, and accessory tenderness before and after treatment using the Pelvic Physical Signs Scale17. The higher the score, the more severe the pelvic pain.</li>
	<li>Use the World Health Organization quality of life (WHOQOL) - BREF scale18 to rate the quality of survival of the patients, and count the domain scores positively, meaning that the higher the score, the better the quality of life.</li>
</ol>
6. Statistical analysis
<ol>
	<li>Express measurement data conforming to a normal distribution as mean &#177; standard deviation (SD).</li>
	<li>Use an independent samples t-test for group comparisons and a paired samples t-test for comparisons before and after treatment.</li>
	<li>Express data not conforming to a normal distribution as M (25%,75%).</li>
	<li>Conduct group comparisons using the rank-sum test and comparisons before and after treatment using the paired rank-sum test.</li>
	<li>Express counts or ordinal data as component ratios.</li>
	<li>Perform group comparisons using Fisher&#39;s exact probability method or the Chi-square test.</li>
	<li>Use a paired-design t-test for pre and post control. Perform bilateral hypothesis testing to &#945;= 0.05; consider P &#60; 0.05 to be statistically significant.</li>
</ol>

Acupoint Catgut Embedding for Chronic Pelvic Pain Management

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H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acupuncture+combined+with+western+medication+on+chronic+pelvic+pain+after+pelvic+inflammatory+disease:+a+multi-center+randomized+controlled+trial.">Acupuncture combined with western medication on chronic pelvic pain after pelvic inflammatory disease: a multi-center randomized controlled trial.</a> Zhongguo Zhen Jiu. 41 (1), 31-35 (2021).</li><li>Xiang, D. F., Sun, Q. Z., Liang, X. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+abdominal+acupuncture+on+pain+of+pelvic+cavity+in+patients+with+endometriosis.">Effect of abdominal acupuncture on pain of pelvic cavity in patients with endometriosis.</a> Zhongguo Zhen Jiu. 31 (2), 113-116 (2011).</li></ol>

The authors declare that they have no conflicts of interest.

There is no record of chronic pelvic pain in ancient Chinese medicine books, and according to its symptom characteristics, it can be mutually referenced with diseases such as &#34;abdominal pain,&#34; &#34;women&#39;s abdominal pain,&#34; and &#34;menstrual abdominal pain.&#34; According to traditional Chinese medicine theory, when the blood vessels are open, improper care may lead to the invasion of pathogenic factors, such as dampness and evil heat, which can stagnate in the uterus and its channels, leading to a strugg...

Chronic pelvic pain (CPP) refers to pelvic pain that persists for over 6 months, is non-periodic, and is unresponsive to non-opioid drugs. The latest research shows that the global incidence rate of CPP is ~5%-26%1. Sequential pelvic inflammatory disease (SPID) is one of the main conditions causing CPP, accounting for 23%-30% of all cases of chronic pelvic pain in females2. Due to the limited efficacy of existing analgesics in alleviating this pain, over 50% of CPP patients may seek non-pharmacological treatment3. Currently, the main non-pharmacological treatments include pelvic floor physical therapy, psychological therapy, neuroregulation, and dietotherapy. However, they also have limitations, such as suboptimal pain relief and difficulties in achieving long-term control. Most SPID women with CPP experience anxiety, depression, and decreased quality of life due to recurrent pain4. Therefore, finding an effective treatment method that can alleviate patients' pain symptoms has become a pressing clinical concern.
Acupuncture and moxibustion, two of the main external therapeutic methods in traditional Chinese medicine, have shown good efficacy in treating pain-related conditions and have been widely used in clinical practice, especially in the relief of chronic pain, where their effectiveness surpasses other non-pharmacological treatments for CPP5,6. In a cross-sectional study on the treatment of CPP with traditional Chinese medicine, 7.63% of chronic pain patients were chosen to receive acupuncture and moxibustion treatment, resulting in excellent therapeutic outcomes7. The acupoint catgut embedding introduced in this article is an extension and development of acupuncture and moxibustion. It refers to a traditional Chinese medicine external treatment technique that uses specially designed disposable medical devices to implant absorbable catgut into corresponding acupoints, providing prolonged and continuous acupoint stimulation to achieve analgesic effect. The author's team has employed this technology to treat recurrent and incurable pelvic pain in women caused by SPID, yielding positive outcomes. It can be utilized either as a standalone treatment or as an important component of a comprehensive traditional Chinese medicine treatment plan or a combination of traditional Chinese and Western medicine. This article takes the most common traditional Chinese medicine syndrome (dampness heat stasis type) patients with SPID as an example to provide a detailed demonstration of the procedures for acupoint catgut embedding.

<table><tbody><tr><td>disposable catgut embedding needles</td><td>Yangzhou Junbang Technology Development Co., Ltd</td><td>20212200112</td><td></td></tr><tr><td>disposable thread embedding kit</td><td>Shangdong Weigao Ruixin Medical Technology Co.,Ltd.</td><td>20182140148</td><td></td></tr><tr><td>SPSS26.0&amp;nbsp;</td><td></td><td></td><td>statistical analysis software&amp;nbsp;</td></tr><tr><td>sterile gloves</td><td>Fitone Latex Products Co.,Ltd Guangdong.</td><td>20172140158</td><td></td></tr></tbody></table>

acupoint catgut embedding for treatment of chronic pelvic pain due to the sequelae of pelvic inflammatory disease

Chronic pelvic pain caused by the sequelae of inflammatory pelvic disease is a common clinical condition of pelvic pain in women. At present, the main challenges in its treatment are the limited effectiveness of pain relief and the frequent recurrence of symptoms, which significantly impact patients' quality of life and impose a considerable psychological burden on them. It is a clinically challenging disease. After summarizing years of treatment experience, the author's team discovered that acupoint catgut embedding demonstrated notable clinical efficacy in managing chronic pelvic pain stemming from pelvic inflammatory disease sequelae. Compared to existing Western medicine treatment methods, acupoint catgut embedding offers advantages such as a good analgesic effect, lower recurrence rate, economic benefits, and a relatively straightforward procedure. This article provides a comprehensive guide on embedding absorbable catgut into patients' acupoints for the treatment of chronic pelvic pain in females resulting from the sequelae of pelvic inflammatory disease.

In this clinical study, a total of 88 participants were initially enrolled, with 8 cases dropping out (4 from the treatment group and 4 from the other one). The remaining 80 participants were divided using a random number table into the treatment and control groups, with 40 participants in each group. The course of treatment included catgut embedding once every 10 days, avoiding menstrual periods, for a continuous duration of 3 months. The patients in the treatment group also took a dose of Traditional Chinese Medicine d...

Read this Scientific Article Protocol about Acupoint Catgut Embedding for Treatment of Chronic Pelvic Pain due to the Sequelae of Pelvic Inflammatory Disease at JoVE.com

Author Spotlight: Enhancing Women's Chronic Pelvic Pain Management Through Acupoint Catgut Embedding

This article outlines acupoint catgut embedding therapy for chronic pelvic pain caused by the sequelae of pelvic inflammatory diseases.

Acupoint Catgut Embedding for Treatment of Chronic Pelvic Pain Due to the Sequelae of Pelvic Inflammatory Disease

Chronic pelvic pain caused by the sequelae of inflammatory pelvic disease is a common clinical condition of pelvic pain in women. At present, the main ...

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Universidad de Cartagena UDEC

Research

JoVE Journal

Medicine

1.1K Views.  Hospital of Chengdu University of Traditional Chinese Medicine. This article outlines acupoint catgut embedding therapy for chronic pelvic pain caused by the sequelae of pelvic inflammatory diseases.

Video: Author Spotlight: Enhancing Women's Chronic Pelvic Pain Management Through Acupoint Catgut Embedding

The Measurement and Treatment of Suppression in Amblyopia

Amblyopia, a developmental disorder of the visual cortex, is one of the leading causes of visual dysfunction in the working age population. Current estimates put the prevalence of amblyopia at approximately 1-3%1-3, the majority of cases being monocular2. Amblyopia is most frequently caused by ocular misalignment (strabismus), blur induced by unequal refractive error (anisometropia), and in some cases by form deprivation.
Although amblyopia is initially caused by abnormal visual input in infancy, once established, the visual deficit often remains when normal visual input has been restored using surgery and/or refractive correction. This is because amblyopia is the result of abnormal visual cortex development rather than a problem with the amblyopic eye itself4,5 . Amblyopia is characterized by both monocular and binocular deficits6,7 which include impaired visual acuity and poor or absent stereopsis respectively. The visual dysfunction in amblyopia is often associated with a strong suppression of the inputs from the amblyopic eye under binocular viewing conditions8. Recent work has indicated that suppression may play a central role in both the monocular and binocular deficits associated with amblyopia9,10 .
Current clinical tests for suppression tend to verify the presence or absence of suppression rather than giving a quantitative measurement of the degree of suppression. Here we describe a technique for measuring amblyopic suppression with a compact, portable device11,12 . The device consists of a laptop computer connected to a pair of virtual reality goggles. The novelty of the technique lies in the way we present visual stimuli to measure suppression. Stimuli are shown to the amblyopic eye at high contrast while the contrast of the stimuli shown to the non-amblyopic eye are varied. Patients perform a simple signal/noise task that allows for a precise measurement of the strength of excitatory binocular interactions. The contrast offset at which neither eye has a performance advantage is a measure of the "balance point" and is a direct measure of suppression. This technique has been validated psychophysically both in control13,14 and patient6,9,11 populations.
In addition to measuring suppression this technique also forms the basis of a novel form of treatment to decrease suppression over time and improve binocular and often monocular function in adult patients with amblyopia12,15,16 . This new treatment approach can be deployed either on the goggle system described above or on a specially modified iPod touch device15.

Amblyopia is a developmental disorder of the visual cortex that is often accompanied by strong suppression of one eye. We present a new technique for measuring and treating interocular suppression in patients with amblyopia that can be deployed using virtual reality goggles or a portable iPod Touch device.

Amblyopia, a developmental disorder of the visual cortex, is one of the leading causes of visual dysfunction in the working age population. Current ...

Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity

Endometriosis is a common disease affecting 40 to 70% of reproductive-aged women with chronic pelvic pain (CPP) and/or infertility. The purpose of this study was to demonstrate the use of a blue dye (methylene blue) to stain peritoneal surfaces during laparoscopy (L/S) to detect the loss of peritoneal integrity in patients with pelvic pain and suspected endometriosis. Forty women with CPP and 5 women without pain were evaluated in this pilot study. During L/S, concentrated dye was sprayed onto peritoneal surfaces, then aspirated and rinsed with Lactated Ringers solution. Areas of localized dye uptake were evaluated for the presence of visible endometriotic lesions. Areas of intense peritoneal staining were resected and some fixed in 2.5% buffered gluteraldehyde and examined by scanning (SEM) electron microscopy. Blue dye uptake was more common in women with endometriosis and chronic pelvic pain than controls (85% vs. 40%). Resection of the blue stained areas revealed endometriosis by SEM and loss of peritoneal cell-cell contact compared to normal, non-staining peritoneum. Affected peritoneum was associated with visible endometriotic implants in most but not all patients. Subjective pain relief was reported in 80% of subjects. Based on scanning electron microscopy, we conclude that endometrial cells extend well beyond visible implants of endometriosis and appear to disrupt the underlying mesothelium. Subtle lesions of endometriosis could therefore cause pelvic pain by disruption of peritoneal integrity, allowing menstrual or ovulatory blood and associated pain factors access to underlying sensory nerves. Complete resection of affected peritoneum may provide a better long-term treatment for endometriosis and CPP. This simple technique appears to improve detection of subtle or near invisible endometriosis in women with CPP and minimal visual findings at L/S and may serve to elevate diagnostic accuracy for endometriosis at laparoscopy.

Loss of peritoneal integrity provides a new paradigm to understand and treat chronic pelvic pain in women with mild forms of endometriosis and can be easily detected using intraoperative instillation of dye at the time of laparoscopy.

Endometriosis is a  common disease affecting 40 to 70% of reproductive-aged women with chronic  pelvic pain (CPP) and/or infertility. The purpose of this ...

Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain

Pelvic pain is a common condition that is associated with dysmenorrhea and endometriosis. In some women the severe episodes of cyclic pain change and the resultant pain becomes continuous and this condition becomes known as Chronic Pelvic Pain. This state can be present even after the appropriate medical or surgical therapy has been instituted. It can be associated with pain and tenderness in the muscles of the abdomen wall and intra-pelvic muscles leading to severe dyspareunia. Additional symptoms of irritable bowel and interstitial cystitis are common. A common sign of the development of this state is the emergence of cutaneous allodynia which emerges from the so-called viscero-somatic reflex. A simple bedside test for the presence of cutaneous allodynia is presented that does not require excessive time or special equipment. This test builds on previous work associated with changes in sensation related to gall bladder function and the viscera-somatic reflex(1;2).
The test is undertaken with the subject s permission after an explanation of how the test will be performed. Allodynia refers to a condition in which a stimulus that is not normally painful is interpreted by the subject as painful. In this instance the light touch associated with a cotton-tipped applicator would not be expected to be painful. A positive test is however noted by the woman as suddenly painful or suddenly sharp. The patterns of this sensation are usually in a discrete pattern of a dermatome of the nerves that innervate the pelvis.
The underlying pathology is now interpreted as evidence of neuroplasticity as a consequence of severe and repeating pain with changes in the functions of the dorsal horns of the spinal cord that results in altered function of visceral tissues and resultant somatic symptoms(3). 
The importance of recognizing the condition lies in an awareness that this process may present coincidentally with the initiating condition or after it has been treated. It also permits the clinician to evaluate the situation from the perspective that alternative explanations for the pain may be present that may not require additional surgery.

Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain

A demonstration of the bedside test for cutaneous allodynia and its clinical implications.

Pelvic pain is a common condition that is associated with dysmenorrhea and endometriosis.  In some women the severe episodes of cyclic pain change and the ...

Biology

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition characterized by increased urgency and frequency of urination, as well as nocturia and general pelvic pain, especially upon bladder filling or voiding. Despite years of research, the cause of IC/BPS remains elusive and treatment strategies are unable to provide complete relief to patients. In order to study nervous system contributions to the condition, many animal models have been developed to mimic the pain and symptoms associated with IC/BPS. One such murine model is urinary bladder distension (UBD). In this model, compressed air of a specific pressure is delivered to the bladder of a lightly anesthetized animal over a set period of time. Throughout the procedure, wires in the superior oblique abdominal muscles record electrical activity from the muscle. This activity is known as the visceromotor response (VMR) and is a reliable and reproducible measure of nociception. Here, we describe the steps necessary to perform this technique in mice including surgical manipulations, physiological recording, and data analysis. With the use of this model, the coordination between primary sensory neurons, spinal cord secondary afferents, and higher central nervous system areas involved in bladder pain can be unraveled. This basic science knowledge can then be clinically translated to treat patients suffering from IC/BPS.

Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition characterized in part by pelvic pain. In order to study nervous system contributions to the condition, a physiological model of urinary bladder pain is used in mice and rats.

Approximately 3-8 million people in the United States suffer from interstitial cystitis/bladder pain syndrome (IC/BPS), a debilitating condition ...

Transvaginal Mesh Insertion in the Ovine Model

This protocol describes mesh insertion into the rectovaginal septum in sheep using a single vaginal incision technique, with and without the trocar-guided insertion of anchoring arms. Parous sheep underwent the dissection of the rectovaginal septum, followed by the insertion of an implant with or without four anchoring arms, both designed to fit the ovine anatomy. The anchoring arms were put in place using a trocar and an "outside-in" technique. The cranial arms were passed through the obturator, gracilis, and adductor magnus muscles. The caudal arms were fixed near the sacrotuberous ligament, through the coccygeus muscles. This technique allows for the mimicking of surgical procedures performed in women suffering from pelvic organ prolapse. The anatomical spaces and elements are easily identified. The most critical part of the procedure is the insertion of the cranial trocar, which can easily penetrate the peritoneal cavity or the surrounding pelvic organs. This can be avoided by a more extensive retroperitoneal dissection and by guiding the trocar more laterally. This approach is designed only for experimental testing of novel implants in large animal models, as trocar-guided insertion is currently not used clinically.

This protocol describes mesh implantation in the ovine rectovaginal septum using a single vaginal incision technique, with and without the trocar-guided insertion of anchoring arms.

This protocol describes mesh insertion into the rectovaginal septum in sheep using a single vaginal incision technique, with and without the trocar-guided ...

Development of Recombinant Proteins to Treat Chronic Pain

Chronic pain is difficult to treat and new approaches to resolve persistent pain are urgently needed. Anti-inflammatory cytokines are promising candidates for treating debilitating pain conditions due to their capacity to regulate aberrant neuro-immune interactions. However, physiologically they work in a network of various cytokines, and therefore their therapeutic effect may not be optimal when used as stand-alone drugs. To overcome this limitation, we developed a fusion protein of the anti-inflammatory cytokines IL4 and IL10. Here, we describe the methods for production and quality control of IL4-10 recombinant fusion protein and we test the effectiveness of the IL4-10 fusion protein to resolve pain in a mouse model of persistent inflammatory pain.

In this paper we provide details for the methods of production and quality control of an IL4-10 recombinant fusion protein. We also show how to test the effectiveness of this protein to resolve pain in a mouse model of inflammatory pain.

Chronic pain is difficult to treat and new approaches to resolve persistent pain are urgently needed. Anti-inflammatory cytokines are promising candidates ...

Acupoint Needle-Embedding Combined with Ironing Therapy for Postoperative Pain After Anal Surgery

Acupoint needle-embedding combined with ironing therapy is a non-drug treatment method to release postoperative pain after anal surgery. The practice is guided by traditional Chinese medicine (TCM) syndrome differentiation theory and employs acupoint stimulation and heat to alleviate pain. Although prior research has shown that these are dependable methods for pain relief, the combined effect of the two techniques has not been described. In our research, we found that compared to using diclofenac sodium enteric-coated capsules alone, adding acupoint needle-embedding combined with ironing therapy was more effective for reducing pain levels at different stages after hemorrhoid surgery. Although this technique is efficient and commonly used in clinics, due to its invasive practice, acupoint needle embedding still carries risks relating to hospital-acquired infections and broken needles. Ironing therapy, on the other hand, can result in burns and connective tissue injuries. Therefore, there is an urgent need to develop a standardized protocol for medical staff. Our protocol refines the traditional techniques and provides detailed instructions on patient preparation, operation techniques, and postoperative care to ensure the therapy is carried out safely and efficiently. By standardizing this therapy, this technique is expected to become an important complementary therapy for postoperative pain relief&#160;in hemorrhoids, which will significantly improve patients &#39;life quality after anal surgery.

The high incidence of pain after anal surgery is due to the physiological structure and pathological status of the anus. At present, the use of painkillers cannot provide satisfactory pain relief, which makes complementary therapy important. This paper proposes acupoint needle-embedding combined with ironing therapy to relieve postoperative pain.

Acupoint needle-embedding combined with ironing therapy is a non-drug treatment method to release postoperative pain after anal surgery. The practice is ...

Acupoint Embedding Therapy for Managing Abdominal Obesity

Acupoint Catgut Embedding Therapy for Abdominal Obesity Treatment

As the global economy grows and living standards improve, the rise in overweight and obese individuals poses a significant public health challenge. This trend is predominantly linked to lifestyle changes and poor dietary habits, resulting in increased abdominal obesity characterized by fat accumulation in the abdominal region and associated symptoms such as indigestion, constipation, and fatigue. Acupoint embedding therapy uses absorbable sutures inserted into specific acupoints for continuous stimulation, aiding in the treatment of abdominal obesity, metabolic issues, and digestive disorders. Its main goals are to regulate metabolism, improve digestion, and reduce fat. This paper offers a thorough exploration of the comprehensive management of thread-embedding acupuncture for obesity treatment. It meticulously outlines critical aspects of patient management and material preparation and provides precise guidance on acupoint selection, surgical procedures, and postoperative care. This structured treatment protocol aims to ensure procedural safety and efficacy, thereby optimizing clinical outcomes for patients with abdominal obesity and ultimately improving their overall quality of life.

This study explores acupoint catgut embedding therapy for obesity, covering patient management, material preparation, acupoint selection, surgery, and post-operative care.

As the global economy grows and living standards improve, the rise in overweight and obese individuals poses a significant public health challenge. This ...

Evaluating Acupoint Catgut Embedding Therapy for Allergic Rhinitis Management

Acupoint Catgut Embedding Therapy in Traditional Chinese Medicine for Managing Allergic Rhinitis

Acupuncture Point Embedding Therapy is a comprehensive treatment method that combines modern biomaterials with acupuncture techniques. Acupoint catgut embedding (ACE) not only avoids the adverse reactions associated with Western medicine but also incorporates the benefits of traditional acupuncture, extending the duration of its therapeutic effects. Allergic rhinitis (AR) is a condition known for its challenging treatment and tendency to persist. This randomized controlled trial included 128 AR patients to evaluate the effectiveness of ACE for AR management. Data were collected at baseline, four weeks after treatment, and eight weeks after treatment. Baseline analysis was performed, and the visual analog scale (VAS) was used as a clinical observation index for this method. The rhinoconjunctivitis quality of life questionnaire (RQLQ) served as a quality of life index. Specific IgE (sIgE), IL-4, IL-10, and IL-12 were used as laboratory observation indices. The results of the experiment validate the clinical effectiveness of ACE in treating AR. Although this technique is efficient and commonly used in clinics, its invasive nature poses risks of hospital-acquired infections. Based on this experiment, this protocol refines traditional techniques, providing detailed instructions on patient preparation, operation techniques, and postoperative care to ensure the safe and effective administration of therapy. By standardizing this therapy, ACE is anticipated to become an important non-drug treatment option for alleviating symptoms in AR patients, thereby significantly enhancing their quality of life.

This protocol details the procedure for acupoint burrowing and demonstrates its effectiveness in treating allergic rhinitis.

Acupuncture Point Embedding Therapy is a comprehensive treatment method that combines modern biomaterials with acupuncture techniques. Acupoint catgut ...

An Acupoint Catgut-embedding Therapy for Treating Obesity

Obesity is a chronic and complex disease caused by excessive fat deposition, which can lead to multiple complications. It affects individuals of all ages and backgrounds, contributing to a growing public health crisis worldwide. In modern medicine, the available treatments for obesity include drugs, behavioral modifications, and surgical procedures. However, their efficacy is often unsatisfactory and may be accompanied by various side effects. In contrast, Traditional Chinese Medicine offers a wide range of treatment options, including herbal tonics, acupuncture, tuina, acupoint catgut-embedding therapy, and more. Among these, acupoint catgut-embedding therapy has emerged as a popular choice for weight management due to its effectiveness, convenience, affordability, and minimal adverse effects. It involves the insertion of absorbable sutures into acupoints, stimulating the body's natural healing processes and enhancing metabolic function. The gradual absorption of the sutures over time provides a sustained therapeutic effect, allowing for prolonged benefits without the need for continuous treatment sessions. Whether used alone or in conjunction with other therapies, acupoint catgut-embedding therapy exemplifies the benefits of integrative medicine and its potential to empower individuals on their journey toward a healthier lifestyle. Aiming to visualize this ethnic medicine theory and promote its effective inheritance, we detail the acupoint catgut-embedding therapy method for weight loss, covering acupuncture points selection, materials, procedures, and precautions.

Here, we propose a specialized Chinese medicine treatment protocol to manage obesity. This treatment achieves weight loss by implanting absorbable surgical sutures into specific acupuncture points.

Obesity is a chronic and complex disease caused by excessive fat deposition, which can lead to multiple complications. It affects individuals of all ages ...

Acupoint Catgut Embedding for Treatment of Chronic Pelvic Pain Due to the Sequelae of Pelvic Inflammatory Disease

Summary

Explore More Videos

The Measurement and Treatment of Suppression in Amblyopia

Intraoperative Detection of Subtle Endometriosis: A Novel Paradigm for Detection and Treatment of Pelvic Pain Associated with the Loss of Peritoneal Integrity

Demonstration of Cutaneous Allodynia in Association with Chronic Pelvic Pain

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

Transvaginal Mesh Insertion in the Ovine Model

Development of Recombinant Proteins to Treat Chronic Pain

Acupoint Needle-Embedding Combined with Ironing Therapy for Postoperative Pain After Anal Surgery

Acupoint Catgut Embedding Therapy for Abdominal Obesity Treatment

Acupoint Catgut Embedding Therapy in Traditional Chinese Medicine for Managing Allergic Rhinitis

An Acupoint Catgut-embedding Therapy for Treating Obesity