Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples
1.0K Views
•
09:26 min
•
June 30th, 2023
DOI :
June 30th, 2023
•José Gonçalves1,2,3, Priscilla Gomes da Silva4,5,6, Tom Koritnik3, Martin Bosilj3, Andrés Torres-Franco1,2, Israel Diaz1,2, Elisa Rodriguéz1,2, Eva Marcos2,3, João R. Mesquita4,5, Pedro García-Encina1,2
1Institute of Sustainable Processes, University of Valladolid, 2Department of Chemical Engineering and Environmental Technology, University of Valladolid, 3Department of Public Health Microbiology, National Laboratory of Health, Environment and Food, 4ICBAS - School of Medicine and Biomedical Sciences, Porto University, 5Epidemiology Research Unit (EPIUnit), Instituto de Saúde Pública da Universidade do Porto, 6Laboratório para a Investigação Integrativa e Translacional em Saúde Populacional (ITR)
Chapters in this video
0:04
Introduction
1:00
Wastewater and Air Samples Collection and Pre‐Processing
1:55
Quantification of SARS‐CoV‐2 RNA by RT‐qPCR
2:46
Sequencing the Variants
7:37
Results: Detection and Quantification of SARS‐CoV‐2 RNA in Wastewater and Air Samples
8:31
Conclusion
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