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Combining Clearing and Fluorescence Microscopy for Visualising Changes in Gene Expression and Physiological Responses to Plasmodiophora brassicae

2.6K Views

06:58 min

August 5th, 2022

DOI :

10.3791/64297-v

August 5th, 2022


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Plasmodiophora Brassicae

Chapters in this video

0:05

Introduction

1:36

Tissue Preparation and Fixation

2:13

Tissue Embedding and Sectioning

3:03

Vibratome Sectioning and Specimen Clearing

4:03

Staining Procedure and Microscopy

4:49

Results: Microscopic Analysis of the Biotrophic Interaction and Gene Expression Regulations Between Plasmodiophora brassicae and Arabidopsis Plants

6:06

Conclusion

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