A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay
4.4K Views
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05:53 min
•
December 7th, 2021
DOI :
December 7th, 2021
•Sebastian Bass-Stringer1,2, Colleen J. Thomas2,3, Clive N. May3, Paul Gregorevic4, Kate L. Weeks1,5,6, Julie R. McMullen1,2,5,6,7
1Baker Heart and Diabetes Institute, 2Department of Physiology, Anatomy and Microbiology, La Trobe University, 3Florey Institute of Neuroscience and Mental Health, University of Melbourne, 4Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, 5Department of Diabetes, Central Clinical School, Monash University, 6Baker Department of Cardiometabolic Health, The University of Melbourne, 7Department of Physiology and Department of Medicine Alfred Hospital, Monash University
Chapters in this video
0:05
Introduction
0:36
Plating and Infecting HT1080 Cells
2:00
Day 3: Fixing and Adding Substrate to The Cells
3:04
Quantitation to Determine the Neutralizing Activity Using ImageJ
3:48
Results: Transduction Assay and Assessment of Neutralizing Antibodies (Nabs) Against AAV6 in Sheep Samples
5:26
Conclusion
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