Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
2.1K Views
•
10:08 min
•
December 21st, 2021
DOI :
December 21st, 2021
•Chapters in this video
0:05
Introduction
0:56
Synthesis of Guide RNAs
2:03
Microinjection of CRISPR-Cas9 Cocktail into a One-Cell Zebrafish Embryo to Generate Maternal Crispants
3:07
Screening for Somatic INDELs in F0 Injected Embryos
4:54
Identification of Maternal-Effect Phenotypes in Maternal Crispant Embryos
6:21
Sequencing Alleles in Maternal Crispant Haploids
8:18
Results: Identification of Maternal Effect Phenotypes in a Rapid and Resource Efficient Manner
9:08
Conclusion
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