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Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies

3.4K Views

12:28 min

March 12th, 2022

DOI :

10.3791/63126-v

March 12th, 2022


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Chapters in this video

0:13

Introduction

1:19

Fluorescent Lectin Statining

3:31

Confocal Microscopy Visualization and Microphotograph Acquisition

4:14

Image Processing

5:31

Quantification of Avascular Areas

6:01

Quantification of Neovascular Areas

6:27

Quantification of Vessel Diameter

6:57

Quantification of Vessel Tortuosity

7:44

Quantification of Vascular Branching

8:16

Quantification of Vascular Density

8:43

Results

12:00

Conclusion

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