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Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

3.5K Views

08:28 min

February 15th, 2022

DOI :

10.3791/63122-v

February 15th, 2022


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Aortic Smooth Muscle Cells

Chapters in this video

0:04

Introduction

0:45

Isolating Primary Human Smooth Muscle Cells (SMCs) from Aortic Biopsies

2:40

Isolating Primary Dermal Fibroblasts from Skin Biopsies

4:25

Measuring Contraction

6:00

Results: Measurement of Aortic SMC Contraction upon Ionomycin Stimulation and SMC Marker Expression

7:51

Conclusion

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