Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts
2.8K Views
•
10:38 min
•
January 8th, 2022
DOI :
January 8th, 2022
•Aleksandra Taran*1,2, Lilia Belikova (Shuvalova)*2,3, Svetlana Lavrushkina1,2, Alexandra Bogomazova3,4, Maria Lagarkova3,4, Irina Alieva1,3
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 3Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 4Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency
Chapters in this video
0:05
Introduction
0:44
Protocol
1:02
Obtaining a Primary Culture of Human Skin Fibroblasts
2:14
Cell Cultivation
4:45
Transfection
6:46
Preparing for Imaging
8:08
Setting the Imaging Parameters
9:45
Results
10:13
Conclusion
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