Fluorescence-Activated Cell Sorting-Radioligand Treated Tissue (FACS-RTT) to Determine the Cellular Origin of Radioactive Signal
2.3K Views
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12:04 min
•
September 10th, 2021
DOI :
September 10th, 2021
•Quentin Amossé1,2, Kelly Ceyzériat1,3,4, Stergios Tsartsalis1,5, Benjamin B. Tournier1,2, Philippe Millet1,2
1Division of Adult Psychiatry, Department of Psychiatry, University Hospitals of Geneva, 2Department of Psychiatry, University of Geneva, 3Division of Nuclear medicine and Molecular Imaging, University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology, University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine, Imperial College London
Chapters in this video
0:04
Introduction
0:38
SPECT Camera Preparation and Calibration
1:47
[125I]CLINDE Radiotracer Synthesis
4:27
SPECT Acquisition
5:15
Scan Reconstruction
5:57
Rat Brain Extraction and Cell Isolation
9:46
Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
11:27
Conclusion
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