Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)
3.5K Views
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08:18 min
•
February 18th, 2022
DOI :
February 18th, 2022
•Xingjin Jian*1,2, Xiaojie Guo*1,2,3, Jia Wang4, Zheng Lin Tan1,2,6, Xin-hui Xing1,2,5, Liyan Wang3, Chong Zhang1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
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Chapters in this video
0:04
Introduction
0:51
Preparations for the Experiment
1:48
Growth Curve Measurement in Microbial Microdroplet Culture System (MMC)
4:25
Adaptive Evolution in Microbial Microdroplet Culture System (MMC)
6:41
Results: The Adaptive Evolution of the Methanol-Essential Escherichia coli (MeSV2.2) in MMC
7:36
Conclusion
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