Determination of Tripartite Interaction between Two Monomers of a MADS-box Transcription Factor and a Calcium Sensor Protein by BiFC-FRET-FLIM Assay
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14:34 min
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December 25th, 2021
DOI :
December 25th, 2021
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Chapters in this video
0:08
Introduction
1:17
Cloning of Genes in Entry and Destination Vectors
2:01
Growth Conditions for Nicotiana benthamiana Plants
3:05
Prepare Bacterial Strains for Agroinfiltration
6:20
Prepare Slides for Fluorescence Visualization
6:45
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope
10:51
Fret-Flim Analysis Using a Confocal Laser Scanning Microscope: The Tripartite Interaction
12:31
Representative Results
13:42
Conclusion
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